Volumetric Pipette

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A Volumetric pipette is a tool for measuring precise volumes of a liquid.

Pipettes are
typically long tubes, open on both ends, marked to contain (or deliver) a certain volume of
liquid.
They are commonly used in analytical chemistry to make up diluted solutions by measuring
out a known volume of the concentrated stock solution, or to measure out a known volume of
a solution to be titrated.
Volumetric pipettes are typically calibrated for only one volume (e.g., 5.00 mL). Many
different sizes are available.
ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The
tolerance depends on the size: a 0.5-mL pipette has a tolerance of 0.006 mL, while a 50-mL
pipette has a tolerance of 0.05 mL. (These are for Class A pipettes; Class B pipettes are
given a tolerance of twice that for the corresponding Class A.)
A specialized example of a volumetric pipette is the
microfluid pipette (capable of dispensing as little as
10L) designed with a circulating liquid tip that
generates a self-confining volume in front of its outlet
channels

Pipetting involves drawing a liquid into a pipet and


allowing liquid to drain from the pipet in a controlled
manner. Pipetting is used to quantitatively transfer a
liquid from one container to another.

IMAGE. A volumetric pipet.

The volumetric pipet has a single graduation that


allows it to deliver one specific volume accurately.
There are many different sizes of volumetric pipets (1-, 5-, 10-, 25-, 50-, and 100-mL
volumetric pipets are common). A volumetric pipet is accurate at the temperature at which it
has been calibrated. The temperature where the volume is accurate is usually printed on the
neck of the pipet. If you are working at a different temperature, the volumetric pipet should
be calibrated.

Graduated pipettes are not as accurate as volumetric pipettes, because each


graduation line is not individually calibrated, and any imperfection in the internal
diameter will have a greater effect on the volume delivered. In volumetric
pipettes, the diameter of the pipette where the graduation mark is located is
significantly smaller because the majority of its volume is located in the bulb.

generally they are used to measure and add a set, precise amount of liquid to a
solution. graduated ones may be used to measure the amount of solution being
added say to determine how much is required to

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