BLAST ACTIVITY:

Shannon Kelso
Biol 230W Lab 011
Accession #: NM_001009860

What species was this mRNA that was sequenced?

-Felis catus galactosidase, beta 1 (GLB1), mRNA

What is the common name of this species?

-House cat

The name of the gene is Fetus catus galactosidase and the length of the
region sequenced is 2322.

This gene codes for the protein beta-galactosidase precursor. The

accession number for the protein is NP_001009860.1.

What conserved domains are present in this protein? Briefly describe

the function of at least two of these domains (if your protein only
has one domain, indicate that below).
-AmyAc_family: catalyzes the transformation of alpha1,4 & alpha1,6
glucosidic links
-BetaGal: has a jelly roll fold

Describe the overall function of this protein. You may need to do

some further searches on your own to determine this!
-This protein cleaves beta-linked galactosyl (terminal) residues from
gangliosides, glycoproteins, & glycosaminoglycans.

Are homologous proteins found in other organisms? If so, what

organism has the most homology and what is the most evolutionary
distant organism that has a protein in the same general protein
family?
-Most: Odobenus rosmarus divergens galactosidase, beta 1 (GLB1), mRNA
-Distant: Nannospalax galili galactosidase, beta 1 (Glb1), mRNA