Exercise No.

2
USE AND CARE OF THE MICROSCOPE
Group No. 3
Section: MRUVWX D
Name of members:
Date Submitted: August 27, 2015
Bernad, Michelle
Ralios, Frances
Ramos, Kiara
Segura, Prince
I.

Data Sheet
See attached Data Sheet

II.

Answers to Questions
1. The resolving power of an objective pertains to its property to
recognize features of a specimen that are close to each other as
separate and distinct (Fernandez, et. al., 2008). The greater the
resolving power, the greater the definition of an object. The resolving
power is dependent on the wavelength of light used and on another
optical property of the objective lens which is the numerical aperture
(Fernandez, et. al., 2008). The numerical aperture (N.A.) is a
measure of the resolving power of the objective (Fernandez, et. al.,
2008).Say, an objective has an N.A. with 0.25, this means the observer
can distinguish 25,000 separate lines per inch. However, if a specimen
has an order of 25,500 or 26,000 lines per inch, the observer can never
see its lines as separate no matter how much magnification is
employed (Fernandez, et. al., 2008). Lenses with higher magnification
usually have higher N.A., but the medium through which the light
passes also affects N.A. (Fernandez, et. al., 2008). N.A. is indicated on
the side of the lens.

III.

Calculations
Calibration of ocular
micrometer under LPO

4 s . 10 m
x
=13.3 m/o
3o . 1s.

Calibration of ocular
micrometer under the
HPO

1 s . 10 m
x
=3.33 m/o
3o . 1s.
Size of specimen (under
HPO)
Entamoeba hystilica cyst
(length x width)

3.33 28 s . =93.24 m (length)

3.33 51 s . =169.83 m (width)

IV.

References
Fernandez, W.L., Dalmacio, A.F., Raymundo, A.K., Zamora, A.F. & Mendoza
B.C. (2008). Laboratory Manual in GENERAL MICROBIOLOGY. 3rd
edition: Microbiology Division, University of the Philippines Los Banos,
College Laguna.