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Purif LacR HisTrap HP 5ml - 21!07!15
Purif LacR HisTrap HP 5ml - 21!07!15
Gel 1 10%SDS-PAGE:
1. LMW
2. Proba dupa expr.
3. Pellet
4. Solubil
5. FT
6. fr. 10
7. 12
8. 14
9. 16
10. 18
11. 22
12. 26
13. 28
14. 30
15. 32
Gel 2 10% SDS-PAGE:
1. LMW
2. fr. 34
3. 36
4. 38
5. 40
6. 42
7. 44
8. 46
9. 50
10. 52
11. 56
12. 62
13. 70
14. 75
15. 80
Probele au stat peste noapte la 4C, fara dializa.
A doua zi, am reunit probele in trei pool-uri:
A. fr. 10 16 (3 ml)
B. fr. 17 31 (7.5ml)
C. fr. 32 83 (25 ml)
Proteina totala Bradford (Ana) (22.07.15):
A. fr. 10 16 (3 ml) conc. prot. 0.12 g/l (total: 0.365 mg/3ml)
B. fr. 17 31 (7ml) conc. prot. 1.698 g/l (total: 12.7 mg/7.5ml)
C. fr. 32 83 (25 ml) conc. prot. 0.43 g/l (total: 10.8 mg/25ml)