Coeus.vn a22.Phụ Gia Tp Chất Điều Vị

CNG HA X HI CH NGHA VIT NAM
QCVN 4-1: 2010/BYT
QUY CHUN K THUT QUC GIA

V PH GIA THC PHM - CHT IU V
National technical regulation
on Food Additive - Flavour Enhancer
H NI - 2010
Li ni u
QCVN 4-1 :2010/BYT do Ban son tho quy chun k
thut quc gia v Ph gia thc phm v cht h tr ch
bin bin son, Cc An ton v sinh thc phm trnh duyt
v c ban hnh theo Thng t s 18/2010/TT-BYT
ngy 20 thng 5 nm 2010 ca B trng B Y t.
QUY CHUN K THUT QUC GIA

V PH GIA THC PHM - CHT IU V
National technical regulation on Food additive Flavour enhancer
I. QUY NH CHUNG
1. Phm vi iu chnh
Quy chun k thut quc gia (sau y gi tt l Quy chun) ny quy nh cc yu cu
k thut v qun l v cht lng, v sinh an ton i vi cc cht iu v c s dng vi
mc ch lm ph gia thc phm.
2. i tng p dng
Quy chun ny p dng i vi:
2.1. T chc, c nhn nhp khu, xut khu, sn xut, bun bn v s dng cc
cht iu v lm ph gia thc phm (sau y gi tt l t chc, c nhn).
2.2. C quan qun l nh nc c lin quan.
3. Gii thch t ng v ch vit tt:
3.1. Cc cht iu v: l ph gia thc phm c s dng vi mc ch tng
hng v ca cc sn phm thc phm.
3.2. JECFA monograph 1 - Vol. 4 (JECFA monographs 1 - Combined
compendium of food addiditive specifications; Joint FAO/WHO expert committee on
food additives; Volume 4 - Analytical methods, test procedures and laboratory
solutions used by and referenced in the food additive specifications; FAO, 2006): Cc
yu cu k thut i vi ph gia thc phm, Tp 4 Cc phng php phn tch, quy
trnh th nghim, dung dch th nghim c s dng (hoc tham chiu) trong yu
cu k thut i vi ph gia thc phm; JECFA bin son; FAO ban hnh nm 2006.
3.3. M s C.A.S (Chemical Abstracts Service): M s ng k ha cht ca
Hip hi Ha cht Hoa K.
3.4. TS (test solution): Dung dch thuc th.
3.5. ADI (Acceptable daily intake): Lng n vo hng ngy chp nhn c.
3.6. INS (International numbering system): H thng m s quc t v ph gia
thc phm.
II. YU CU K THUT, PHNG PHP TH V LY MU
1. Yu cu k thut v phng php th i vi cc cht iu v c quy nh
ti cc ph lc ban hnh km theo Quy chun ny nh sau:
1.1.
Ph lc 1:
Yu cu k thut v phng php th i vi acid LGlutamic
QCVN 4-1 : 2010/BYT

1.2.
Ph lc 2:
Yu cu k thut v phng php th i vi mononatri

L-glutamat
1.3.
Ph lc 3:
Yu cu k thut v phng php th i vi monokali

L-glutamat
1.4.
Ph lc 4:
Yu cu k thut v phng php th i vi calci di-Lglutamat
1.5.
Ph lc 5:
Yu cu k thut v phng php th i vi acid 5'guanylic
1.6.
Ph lc 6:
Yu cu k thut v phng php th i vi acid 5'inosinic
1.7.
Ph lc 7:
Yu cu k thut v phng php th i vi maltol
1.8.
Ph lc 8:
Yu cu k thut v phng php th i vi ethyl

maltol
2. Cc yu cu k thut quy nh trong Quy chun ny c th theo JECFA

monograph 1 - Vol. 4, ngoi tr mt s php th ring c m t trong cc ph lc.
Cc phng php th c hng dn trong Quy chun ny khng bt buc phi
p dng, c th s dng cc phng php th khc tng ng.
3. Ly mu theo hng dn ti Thng t 16/2009/TT-BKHCN ngy 02 thng 6

nm 2009 ca B Khoa hc v Cng ngh v hng dn kim tra nh nc v cht
lng hng ha lu thng trn th trng v cc quy nh khc ca php lut c lin
quan.
III. YU CU QUN L
1. Cng b hp quy
1.1. Cc cht iu v phi c cng b ph hp vi cc quy nh ti Quy
chun ny.
1.2. Phng thc, trnh t, th tc cng b hp quy c thc hin theo Quy
nh v chng nhn hp chun, chng nhn hp quy v cng b hp chun, cng
b hp quy ban hnh km theo Quyt nh s 24/2007/Q-BKHCN ngy 28 thng 9
nm 2007 ca B trng B Khoa hc v Cng ngh v cc quy nh ca php lut.
2. Kim tra i vi cht iu v
Vic kim tra cht lng, v sinh an ton i vi cc cht iu v phi thc hin
theo cc quy nh ca php lut.
QCVN 4-1 : 2010/BYT

IV. TRCH NHIM CA T CHC, C NHN
1. T chc, c nhn phi cng b hp quy ph hp vi cc quy nh k
thut ti Quy chun ny, ng k bn cng b hp quy ti Cc An ton v sinh thc
phm v bo m cht lng, v sinh an ton theo ng ni dung cng b.
2. T chc, c nhn ch c nhp khu, xut khu, sn xut, bun bn v s dng
cc cht iu v sau khi hon tt ng k bn cng b hp quy v bo m cht lng,
v sinh an ton, ghi nhn ph hp vi cc quy nh ca php lut.
V. T CHC THC HIN
1. Giao Cc An ton v sinh thc phm ch tr, phi hp vi cc c quan chc
nng c lin quan hng dn trin khai v t chc vic thc hin Quy chun ny.
2. Cn c vo yu cu qun l, Cc An ton v sinh thc phm c trch nhim
kin ngh B Y t sa i, b sung Quy chun ny.
3. Trng hp hng dn ca quc t v phng php th v cc quy nh
ca php lut vin dn trong Quy chun ny c sa i, b sung hoc thay th th
p dng theo vn bn mi.
QCVN 4-1 : 2010/BYT

Ph lc 1
YU CU K THUT V PHNG PHP TH
I VI ACID L-GLUTAMIC
1. Tn khc, ch s
L-glutamic acid, Glutamic acid

INS 620
ADI khng gii hn
2. nh ngha
Tn ha hc
Acid L-glutamic; Acid L-(+)-glutamic, acid L-2-aminopentandioic; Acid L-alpha-aminoglutaric
M s C.A.S.
56-86-0
Cng thc phn t
C5H9NO4
Cng thc cu to
HO
OH
NH2
Khi lng phn t
147,13
3. Cm quan
Tinh th hoc bt tinh th mu trng, hoc khng mu.
4. Chc nng
Cht iu v, thay th mui.
5. Yu cu k thut
5.1. nh tnh
tan
t tan trong nc, thc t khng tan trong ethanol v ether.
Glutamat
Phi c phn ng c trng ca glutamat.
5.2. tinh khit

Gim khi lng khi lm Khng c qu 0,2%
(Sy ti 80 oC trong 3 gi).
kh
pH
Dung dch bo ha mu th c pH trong khong 3,0 - 3,5.
Gc quay cc ring
Dung dch mu th 10% (kl/tt) trong acid hydrocloric 2 N c

[ ]D20 trong khong +31,5 n +32,2o
Tro sulfat
Khng c qu 0,2%.
Clorid
Khng c qu 0,2%.
Acid pyrrolidon
carboxylic
t yu cu (m t trong phn phng php th).
Arsen
Khng c qu 3,0 mg/kg
QCVN 4-1 : 2010/BYT

Ch
Khng c qu 1,0 mg/kg.
5.3. Hm lng
C5H9NO4
Khng thp hn 99,0% tnh theo ch phm lm kh.
6. Phng php th
6.1 tinh khit
Tro sulfat
Th theo JECFA monograph 1 - Vol.4 - Phng php I,

cn 1 g mu.
Clorid
Th theo JECFA monograph 1 - Vol.4 - Cn 0,07 g mu

th, tin hnh theo hng dn trong chuyn lun th gii
hn clorid, s dng 0,4 ml dung dch acid hydrocloric 0,01
N lm mu chng.
Acid pyrrolidon
carboxylic
- Th theo JECFA monograph 1 - Vol.4. Phng php sc

k bn mng.
- Mu th : Chun b dung dch mu th 0,5%. Ly 2 l.
- Chun : pha dung dch chun mononatri glutamat 0,5%
cha 2,5 mg acid pyrrolidon carboxylic.
- Dung mi: hn hp n-butanol/acid acetic bng/nc, t l
th tch 2/1/1.
- Pha tnh hp ph : Silicagel.
Dung dch h tinh bt-kali iodid : un nng 0,5 g tinh bt
trong 50 ml nc khuy u n khi h ha. ngui,
thm 0,5 g kali iodid v thm nc n 100 ml.
- Tin hnh chy sc k theo hng dn trong chuyn lun
sc k bn mng (cc phng php phn tch). Dng qu
trnh sc k khi tuyn dung mi cch im xut pht
khong 10 cm, kh dung mi trong khng kh, khong
30 pht.
- ng thi, chun b bung sc k tng t, t vo 1 cc
thy tinh dung tch 50 ml, cho vo cc 3 g natri hypoclorid,
thm t t vo cc 1 ml acid hydrocloric to kh clor,
y np v yn trong 30 giy to mi trng kh clor
trong bung. t bn mng kh vo bung sc k,
y np v yn trong 20 pht. Ly bn mng ra khi
bung, yn 10 pht trong khng kh v xt ethanol ln
bn mng. Sau khi kh, xt dung dch h tinh bt-kali
iodid ln bn mng, quan st di nh sng thng ngay
sau khi vt chun xut hin.
Yu cu : Trong sc k ca mu th khng c c vt
tng ng vt acid pyrrolidon carboxylic trong sc k
ca chun ( nhy 0,2%).
Arsen
- Th JECFA monograph 1 - Vol.4, Phng php II.
Ch
- Th theo JECFA monograph 1 - Vol.4.

- Xc nh bng k thut hp th nguyn t thch hp cho
hm lng quy nh. La chn c mu th v phng
5
QCVN 4-1 : 2010/BYT

php chun b mu da trn nguyn tc ca phng php
m t ti JECFA monograph 1 - Vol.4 phn cc phng
php phn tch cng c.
6.2. nh lng
Cn khong 200 mg (chnh xc n mg) mu th c
lm kh trc, ha tan trong 6 ml acid formic, thm 100 ml
acid acetic bng. Chun vi dung dch acid percloric 0,1
N, xc nh im kt thc chun bng o th. Tin hnh
lm mu trng song song hiu chnh nn.
Mi ml dung dch acid percloric 0,1 N tng ng vi
14,713 mg C5H9NO4.
QCVN 4-1 : 2010/BYT

Ph lc 2
I VI MONONATRI L-GLUTAMAT
1. Tn khc, ch s
Monosodium L-glutamate, Sodium glutamate

INS 621
ADI khng gii hn
2. nh ngha
Tn ha hc
Mononatri L-glutamat monohydrat; Mui mononatri ca acid

glutamic, monohydrat
M s C.A.S.
6106-04-3
Cng thc phn t
C5H8NNaO4.H2O
Cng thc cu to
NH2
HO
O
O
Na +
Khi lng phn t
187,13
3. Cm quan
Tinh th hoc bt tinh th trng, thc t khng mi.
4. Chc nng
Cht iu v.
5. Yu cu k thut
5.1. nh tnh
tan
D tan trong nc, t tan trong ethanol, thc t khng tan

trong ether.
Glutamat
Natri
Phi c phn ng c trng ca natri.
5.2. tinh khit

Gim khi lng khi lm
kh
Khng c qu 0,5% (Sy ti 98oC trong 5 gi).
pH
Dung dch mu th 1/50 c pH trong khong 6,7 - 7,2.
Gc quay cc ring

[ ]D20 trong khong +24,8 n +25,3o
Clorid
Khng c qu 0,2%.
Acid pyrrolidon carboxylic
Ch
QCVN 4-1 : 2010/BYT

5.3. Hm lng
C5H8NNaO4.H2O
6. Phng php th
6.1 tinh khit
Clorid
Th theo JECFA monograph 1 - Vol.4 - Cn 0,07 g mu

th, tin hnh theo hng dn trong chuyn lun th gii
hn clorid, s dng 0,4 ml dung dch acid hydrocloric 0,01
N lm mu chng.

k bn mng.
- Chun : pha dung dch chun mono natri glutamat 0,5%
th tch 2/1/1.
- Pha tnh hp th : Silicagel.
30 pht.
thm t t vo cc 1 ml acid hydrocloric to kh clor, y
np v yn trong 30 giy to mi trng kh clor
Ch

php phn tch cng c.
6.2. nh lng
8
QCVN 4-1 : 2010/BYT

lm mu trng song song hiu chnh nn.
9,356 mg C5H9NNaO4.H2O.
QCVN 4-1 : 2010/BYT

Ph lc 3
I VI MONOKALI L-GLUTAMAT
1. Tn khc, ch s
Monopotassium L-glutamate, Potassium glutamate; MPG

INS 622
ADI khng gii hn
2. nh ngha
Tn ha hc
Monokali L-glutamat monohydrat; Mui monokali ca acid

glutamic monohydrat
M s C.A.S.
19473-49-5
Cng thc phn t
C5H8NKO4.H2O
Cng thc cu to
NH2
HO
O
O
Khi lng phn t
K+
203,24
3. Cm quan
4. Chc nng
5. Yu cu k thut
5.1. nh tnh
tan
D tan trong nc, t tan trong ethanol.
Glutamat
Kali
Phi c phn ng c trng ca kali.
5.2. tinh khit

Gim khi lng khi lm
kh
Khng c qu 0,2% (Sy ti 80oC trong 5 gi).
pH
Dung dch mu th 1/50 c pH trong khong 6,7 - 7,3.
Gc quay cc ring

[ ]D20 trong khong +22,5 n +24,0o
Clorid
Khng c qu 0,2%.
Ch
10
QCVN 4-1 : 2010/BYT

5.3. Hm lng
C5H8NKO4.H2O
6. Phng php th
6.1 tinh khit
Clorid
- Th theo JECFA monograph 1 - Vol.4

- Cn 0,07 g mu th, tin hnh theo hng dn trong
chuyn lun th gii hn clorid, s dng 0,4 ml dung dch
acid hydrocloric 0,01 N lm mu chng.

k bn mng.
- Dung mi: hn hp n-butanol/acid acetic bng/nc, theo
t l th tch tng ng 2/1/1.
30 pht.
Ch
- Th theo JECFA monograph 1 - vol. 4

php phn tch cng c.
11
QCVN 4-1 : 2010/BYT

6.2. nh lng
lm mu trng song song hiu chnh nn
10,162 mg C5H8KNO4.H2O.
12
QCVN 4-1 : 2010/BYT

Ph lc 4
I VI CALCI di-L-GLUTAMAT
1. Tn khc, ch s
Monocalcium di-L-glutamate, Calcium glutamate;

INS 623
ADI khng gii hn
2. nh ngha
Tn ha hc
Monocalci di-L-glutamat;
M s C.A.S.
19238-49-4
Cng thc phn t
C10H16CaN2O8 . xH2O (x = 0, 1, 2, 6 hoc 4)
Cng thc cu to
NH2
HO
O
O
Ca + +
O
2
Khi lng phn t
332,32 (dng khan)
3. Cm quan
Tinh th hoc bt tinh th trng, thc t khng mi.
4. Chc nng
5. Yu cu k thut
5.1. nh tnh
tan
D tan trong nc.
Glutamat
Calci
Phi c phn ng c trng ca calci.
5.2. tinh khit

Nc
Khng c qu 19,0%
Gc quay cc ring

[ ]D20 trong khong +27,4 n +29,2o
Clorid
Khng c qu 0,2%.
Ch
5.3. Hm lng
C10H16CaN2O8
Khng thp hn 98,0% v khng c qu 102,0% tnh

theo ch phm khan.
13
QCVN 4-1 : 2010/BYT

6. Phng php th
6.1 tinh khit
Nc

- Phng php Karl Fischer.
Clorid

- Cn 0,07 g mu th, tin hnh theo hng dn trong
chuyn lun th gii hn clorid, s dng 0,4 ml dung dch
acid hydrocloric 0,01 N lm mu chng.

k bn mng.
th tch 2/1/1.
30 pht.
Ch

php phn tch cng c.
14
QCVN 4-1 : 2010/BYT

6.2. nh lng
lm mu trng song song hiu chnh nn
8,308 mg C10H16CaN2O8. Tnh hm lng da trn ch
phm khan.
15
QCVN 4-1 : 2010/BYT

Ph lc 5
YU CU K THUT V PHNG PHP TH I VI ACID 5-GUANYLIC
1. Tn khc, ch s
5-Guanylic acid; Guanylic acid; GMP

INS 626
ADI khng gii hn
2. nh ngha
Tn ha hc
Acid Guanosin-5-monophosphoric
M s C.A.S.
85-32-5
Cng thc phn t
C10H14N5O8P
Cng thc cu to
Khi lng phn t
363,22
3. Cm quan
Tinh th trng hoc khng mu, bt tinh th trng; khng

mi
4. Chc nng
Cht iu v
5. Yu cu k thut
5.1. nh tnh
tan
t tan trong nc, thc t khng tan trong ethanol.
Ph hp th quang
Dung dch mu th 1/50000 trong acid hydrocloric 0,01 N

c cc i hp th ti 256 2 nm. T s A256/A260 trong
khong t 0,95 n 1,03 v t s A280/A260 trong khong t
0,63 n 0,71.
Ribose
Phi c phn ng c trng ca ribose.
Phosphat hu c
Phi c phn ng c trng ca phosphat hu c.
5.2. tinh khit

Gim khi lng khi lm
kh
Khng c qu 1,5%
(sy 120 oC trong 4 gi).
pH
Dung dch mu th 1/400 c pH t 1,5 n 2,5 .
16
QCVN 4-1 : 2010/BYT

Cc tp cht ngoi lai c
lin quan
Khng pht hin bng phng php sc k.
Ch
5.3. Hm lng
C10H14N5O8P

theo ch phm lm kh.
6. Phng php th
6.1. nh tnh
Phosphat hu c
- Th theo hng dn ti JECFA monograph 1 - Vol.4

- Dch th l dung dch mu th 1/400.
6.2. tinh khit

lin quan
Ch
Th theo hng dn ti JECFA monograph 1 - Vol.4. Xc

nh bng k thut hp th nguyn t thch hp cho hm
lng quy nh. La chn c mu th v phng php
chun b mu da trn nguyn tc ca phng php m t
ti JECFA monograph 1 - Vol.4 phn cc phng php
phn tch cng c.
- dng 2 l dung dch mu th 1/400.
6.3. nh lng
Cn khong 0,5 g (chnh xc n 0,1 mg) mu th ha tan
trong acid hydrocloric 0,01 N v nh mc n 1000 ml. ly
10 ml dung dch ny pha long vi acid hydrocloric 0,01 N
va thu c 250 ml. Xc nh hp th quang ca
dung dch ny trong cuvet 1 cm ti bc sng 260 nm v
s dng dung dch acid hydrocloric 0,01 N lm mu chng.
Tnh hm lng % C10H14N5O8P trong mu th theo cng
thc sau:
%=
A X
235
250000
Khi lng mu
(mg)
X
100
100
100- gim khi lng khi
lm kh (%)
17
QCVN 4-1 : 2010/BYT

Ph lc 6
YU CU K THUT V PHNG PHP TH I VI ACID 5-INOSINIC
1. Tn khc, ch s
5- Inosinic acid; Inosinic acid; IMP

INS 630
ADI khng gii hn
2. nh ngha
Tn ha hc
Acid Inosin-5-monophosphoric
M s C.A.S.
131-99-7
Cng thc phn t
C10H13N4O8P
Cng thc cu to
Khi lng phn t
348,21
3. Cm quan
Tinh th trng hoc khng mu, bt tinh th trng; khng

mi
4. Chc nng
Cht iu v
5. Yu cu k thut
5.1. nh tnh
tan
D tan trong nc, t tan trong ethanol.
Ph hp th quang
Dung dch mu th 1/50000 trong acid hydrocloric 0,01 N

c cc i hp th ti 250 2 nm. T s A250/A260 trong
khong t 1,55 n 1,65 v t s A280/A260 trong khong t
0,20 n 0,30.
Ribose
Phi c phn ng c trng ca ribose.
Phosphat hu c
Phi c phn ng c trng ca phosphat hu c.
5.2. tinh khit

Gim khi lng khi lm
kh
Khng c qu 3,0% (sy ti 120 oC trong 4 gi).
pH
Dung dch mu th 1/20 c pH t 1,0 n 2,0.

lin quan
Khng pht hin bng phng php sc k.
18
QCVN 4-1 : 2010/BYT

Ch
5.3. Hm lng
C10H13N4O8P

theo ch phm lm kh.
6. Phng php th
6.1. nh tnh
Phosphat hu c

- Dch th l dung dch mu th 1/400.
6.2. tinh khit

lin quan
Ch
- Th theo hng dn ti JECFA monograph 1 - Vol.4.

m t trong JECFA monograph 1 - Vol.4 phn cc phng
php phn tch cng c.
- dng 1 l dung dch mu th 1/200.
6.3. nh lng
Cn khong 0,5 g (chnh xc n 0,1 mg) mu th ha tan
trong acid hydrocloric 0,01 N v nh mc n 1000 ml. ly
10 ml dung dch ny pha long vi acid hydrocloric 0,01 N
va thu c 250 ml. Xc nh hp th quang ca
dung dch trong cuvet 1 cm ti bc sng 250 nm v s
dng dung dch acid hydrocloric 0,01 N lm mu chng.
Tnh hm lng % C10H13N4O8P trong mu th theo cng
thc sau:
%=
A
349
250000
Khi lng mu
(mg)
100
100- gi tr gim khi
lng khi lm kh (%)
100
19
QCVN 4-1 : 2010/BYT

Ph lc 7
YU CU K THUT V PHNG PHP TH I VI MALTOL
1. Tn khc, ch s
2. nh ngha
INS 636
ADI = 0 - 1 mg/kg th trng
Maltol thu c bng cch tng hp ha hc
Tn ha hc
3-hydroxy-2-methyl-4-pyron
M s C.A.S.
118-71-8
Cng thc ha hc
C6H6O3
Cng thc cu to
Khi lng phn t
126,11
3. Cm quan
Bt tinh th trng c mi caramel b ngo ng.
4. Chc nng
Cht iu v, tr hng, hng liu.
5. Yu cu k thut
5.1. nh tnh
tan
t tan trong nc, tan trong ethanol v propylen glycol.
Khong nng chy
160o - 164o.
Hp th t ngoi
Ph hp th t ngoi ca dung dch mu th 10 mg/l trong

acid hydrocloric 0,1 N c 1 cc i hp th ti 274 nm.
5.2. tinh khit

Nc
Khng c qu 0,5%
Tro sulfat
Khng c qu 0,2%. (Cn 5 g mu).
Ch
5.3. Hm lng C6H6O3
Khng thp hn 99,0% tnh theo ch phm khan.
6. Phng php th
6.1. tinh khit
Nc

- Phng php Karl-Fischer.
Tro sulfat

20
QCVN 4-1 : 2010/BYT

- Mu th: Cn 5 g .
Ch

- Xc nh bng k thut AAS-ICP/AES thch hp cho hm
ti JECFA monograph 1 - Vol.4 phn Cc phng php
chung, tp cht kim loi.
6.2. nh lng
Dung dch chun
Cn 50 mg (chnh xc) chun Maltol USP, cho vo bnh
nh mc 250 ml, ha tan v nh mc n vch bng acid
hydrocloric 0,1 N v lc u. Dng pipet ly 5 ml cho vo
bnh nh mc 100 ml, pha long n vch bng acid
hydrocloric 0,1 N, lc u.
Dung dch nh lng
Cn 50 mg (chnh xc) mu th, cho vo bnh nh mc
250 ml, ha tan v nh mc n vch bng acid
Tin hnh th
Xc nh hp th quang ca dung dch chun v dung
dch nh lng ti 274 nm s dng cuvet o 1 cm bng
thch anh, acid hydrocloric 0,1 N l mu trng.
Tnh hm lng % ca maltol trong mu th theo cng
thc sau:
% Maltol = 100 x W S x AA / (AS x WA)
Trong :
AA l hp th quang ca dung dch mu (dung dch nh
lng)
AS l hp th quang ca dung dch chun
WA l khi lng mu trong dung dch nh lng (mg)
WS l khi lng chun trong dung dch chun (mg)
21
QCVN 4-1 : 2010/BYT

Ph lc 8
YU CU K THUT V PHNG PHP TH I VI ETHYL MALTOL
1. Tn khc, ch s
2. nh ngha
INS 637
ADI = 0 - 2 mg/kg th trng
Ethyl maltol thu c bng cch tng hp ha hc
Tn ha hc
2-Ethyl-3-hydroxy-4-pyron
M s C.A.S.
4940-11-8
Cng thc ha hc
C7H8O3
Cng thc cu to
Khi lng phn t
140,14
3. Cm quan
Bt tinh th trng c mi ko bng.
4. Chc nng
Cht iu v, tr hng, hng liu.
5. Yu cu k thut
5.1. nh tnh
tan
t tan trong nc, tan trong ethanol v propylen glycol.
Khong nng chy
89o - 93o.
Hp th t ngoi
Ph hp th t ngoi ca dung dch mu th 10 mg/l trong

acid hydrocloric 0,1 N c 1 cc i hp th ti 276 nm.
5.2. tinh khit

Nc
Khng c qu 0,5% .
Tro sulfat
Khng c qu 0,2%.
Ch
5.3. Hm lng C7H8O3
Khng thp hn 99,0% tnh theo ch phm khan.
6. Phng php th
6.1 tinh khit
Nc

- Phng php Karl Fischer
Tro sulfat

22
QCVN 4-1 : 2010/BYT

- Cn 5 g mu
Ch

- Xc nh bng k thut AAS-ICP/AES thch hp cho hm
ti JECFA monograph 1 - Vol.4 phn Cc phng php
chung, tp cht kim loi.
6.2. nh lng
Dung dch chun
Cn 50 mg (chnh xc) chun Ethyl maltol USP, cho vo
bnh nh mc 250 ml, ha tan v pha long n vch bng
acid hydrocloric 0,1 N v lc u. Dng pipet ly 5 ml cho
vo bnh nh mc 100 ml, pha long n vch bng acid
Dung dch mu th
Cn 50 mg (chnh xc) mu th, cho vo bnh nh mc
250 ml, ha tan v pha long n vch bng acid
Tin hnh th
Xc nh hp th quang ca dung dch chun v dung
dch mu th ti 276 nm s dng cuvet o 1-cm bng
thch anh, acid hydrocloric 0,1 N l mu trng.
Tnh hm lng % ca Ethyl maltol trong mu th theo
cng thc sau:
% Ethyl maltol = 100 x W S x AA / (AS x W A)
Trong :
AA l hp th quang ca dung dch mu th
AS l hp th quang ca dung dch chun
WA l khi lng mu trong dung dch nh lng (mg)
WS l khi lng chun trong dung dch chun (mg)
23

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CNG HA X HI CH NGHA VIT NAM

QCVN 4-1: 2010/BYT

QUY CHUN K THUT QUC GIA

QUY CHUN K THUT QUC GIA

Yu cu k thut v phng php th i vi acid LGlutamic

QCVN 4-1 : 2010/BYT

Yu cu k thut v phng php th i vi mononatri

Yu cu k thut v phng php th i vi monokali

Yu cu k thut v phng php th i vi calci di-Lglutamat

Yu cu k thut v phng php th i vi acid 5'guanylic

Yu cu k thut v phng php th i vi acid 5'inosinic

Yu cu k thut v phng php th i vi maltol

Yu cu k thut v phng php th i vi ethyl

2. Cc yu cu k thut quy nh trong Quy chun ny c th theo JECFA

3. Ly mu theo hng dn ti Thng t 16/2009/TT-BKHCN ngy 02 thng 6

QCVN 4-1 : 2010/BYT

QCVN 4-1 : 2010/BYT

L-glutamic acid, Glutamic acid

Acid L-glutamic; Acid L-(+)-glutamic, acid L-2-aminopentandioic; Acid L-alpha-aminoglutaric

Cng thc phn t

Khi lng phn t

Tinh th hoc bt tinh th mu trng, hoc khng mu.

Cht iu v, thay th mui.

t tan trong nc, thc t khng tan trong ethanol v ether.

Phi c phn ng c trng ca glutamat.

5.2. tinh khit

Dung dch bo ha mu th c pH trong khong 3,0 - 3,5.

Dung dch mu th 10% (kl/tt) trong acid hydrocloric 2 N c

t yu cu (m t trong phn phng php th).

Khng c qu 3,0 mg/kg

QCVN 4-1 : 2010/BYT

Khng c qu 1,0 mg/kg.

Khng thp hn 99,0% tnh theo ch phm lm kh.

Th theo JECFA monograph 1 - Vol.4 - Phng php I,

Th theo JECFA monograph 1 - Vol.4 - Cn 0,07 g mu

- Th theo JECFA monograph 1 - Vol.4. Phng php sc

- Th JECFA monograph 1 - Vol.4, Phng php II.

- Th theo JECFA monograph 1 - Vol.4.

QCVN 4-1 : 2010/BYT

QCVN 4-1 : 2010/BYT

Monosodium L-glutamate, Sodium glutamate

Mononatri L-glutamat monohydrat; Mui mononatri ca acid

Cng thc phn t

Khi lng phn t

Tinh th hoc bt tinh th trng, thc t khng mi.

D tan trong nc, t tan trong ethanol, thc t khng tan

Phi c phn ng c trng ca glutamat.

Phi c phn ng c trng ca natri.

5.2. tinh khit

Khng c qu 0,5% (Sy ti 98oC trong 5 gi).

Dung dch mu th 1/50 c pH trong khong 6,7 - 7,2.

Dung dch mu th 10% (kl/tt) trong acid hydrocloric 2 N c

Acid pyrrolidon carboxylic

t yu cu (m t trong phn phng php th).

Khng c qu 1,0 mg/kg.

QCVN 4-1 : 2010/BYT

Khng thp hn 99,0% tnh theo ch phm lm kh.

Th theo JECFA monograph 1 - Vol.4 - Cn 0,07 g mu

Acid pyrrolidon carboxylic