BIO/BCH 352

Fall 2015

Name__________________________________ ID no. __________________ Rec sec _______

352 Genetics Homework 9 (10 pts)
1. (2 pts) DNA replication involves a variety of enzymes. Describe the specific role of the enzymes
below during DNA replication in one brief sentence (i.e., not simply that type of enzymes activity).
DNA polymerase III

DNA polymerase I

Primase

DNA ligase

2. (6 pts) Below is a diagram of a DNA molecule that is undergoing bidirectional replication. Label
the following on the diagram:
primers
DNA helicase
Okazaki fragments
DNA gyrase
Site of action of the following enzymes:
*Be sure to indicate the polarities (5 3) of
DNA polymerase I
the daughter strands
DNA polymerase III
*Be sure to label where replication is
DNA ligase
continuous or discontinuous

BIO/BCH 352

Fall 2015

3. (2 points) On the blank replication forks below, draw the results of DNA synthesis when each
enzyme listed is mutated and rendered non-functional but the others are normal. Use different
colors for DNA and RNA (or clearly label them) on the diagrams.
DNA Polymerase I mutant

Primase mutant

DNA Polymerase III mutant

DNA Ligase mutant