Lecture 7:

Gene Cloning and Analysis

Part II

Cloning essentials
1. DNA that contains the desired gene
(insert)
2. Plasmid (vector)
3. Competent cells (host)

Can a single E.coli take up two plasmids?

Plasmid incompatibility is defined as the failure
of two plasmids co-resident in the same cell to
be stably inherited
Plasmids with similar origins of replication, and
therefore similar systems for regulating the
replication and segregation of the plasmid, are
said to be in the same "incompatibility group"
and cannot both be passed down consistently to
daughter cells

Discussion
Gene Cloning Procedures