Fluorescence Experiment

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Concentration of Mg2+,

ppm
Standard Solutions

Fluorescence intensity
1
2
3
5
8

308.5666667
333.8666667
463.9333333
498.2666667
577.2
109.3333333
368.3

Solvent
Unknown

Fluorescence Intensity
vs Concentration of Mg2+
Fluorescence Intensity

700
y = 40.454x + 267.66
R = 0.9758

600
500
400
300
200
100
0
0

Concentration of Mg2+, ppm

Slope
Y-intercept
Coefficient of Regression

max, nm
Excitation
689
Emission
520

40.454
267.66
0.9758

10

Concentration of Mg2+,
ppm

Cycle
1

Cycle

Fluorescence intensity
1
107.4
2
106.6
3
114.0

Cycle

Fluorescence intensity
1
368.8
2
367.8
3
368.3

1
2
3
1
2
3
1
2
3
1
2
3
1
2
3

Fluorescence intensity
309.5
309.2
307.0
333.6
334.4
333.6
464.6
463.5
463.7
501.1
498.3
495.4
577.8
577.0
576.8

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