Poroshell 120 1290

Minjia HUANG

Poroshell 120 EC-C18 1290 (LC)

Agilent Technologies, Inc.

(azo colorant) 22 (aromatic amine)

100102 3

4 14 26 (aniline)

Rongjie FU

200131 412

-N=N-

(EU)
EN14362 [1, 2] 1 22
141314

3.0 150mm2.7 m Poroshell 120 EC-C18

1290 22 4 (1,
4-phenylendiamine aniline 2,6-dimethylaniline 2,4-dimethylaniline)
EN 14362

1. 141314

(CAS)

1,4-phenylendiamine

106-50-3

1290 LC

4-methoxy-m-phenylenediamine

615-05-4

(TCC)

4-methyl-m-phenylenediamine

95-80-7

(DAD)

(Aniline)

62-53-3

benzidine

92-87-5

o-anisidine
2-methoxyaniline

90-04-0

4,4'-oxydianiline

101-80-4

o-toluidine
2-aminotoluene

95-53-4

4-chloroaniline

106-47-8

10

5-nitro-o-toluidine

99-55-8

11

4,4'-methylenedianiline
4,4'-diaminodiphenylmethane

101-77-9

12

3.0 150 mm2.7 m Poroshell 120 ECC18 (p/n: 693975-302)

1 26 J&K
A (10:90)
12 g/mL 4 C

HPLC

Poroshell 120 EC-C18 3.0 mm 150 mm2.7 m

(p/n: 693975-302)

40 C

6-methoxy-m-toluidine
p-cresidine

120-71-8

0.8 mL

13

2,6-dimethylaniline

87-62-7

14

2,4-dimethylaniline

95-68-1

15

3,3'-dimethoxybenzidine
o-dianisidine

119-90-4

A0.575 g (Mono ammonium phosphate)

0.7 g (Disodium hydrogen phosphate)
900 mL 100 mL pH
pH 6.9
B

3,3'-dimethylbenzidine
4,4'-bi-o-toluidine

DAD

240 nm280 nm305 nm

119-93-7

1 L

17

4,4'-thiodianiline

139-65-1

18

2-naphthylamine

91-59-8

19

4-chloro-o-toluidine

95-69-2

20

2,4,5-trimethylaniline

137-17-7

21

4,4'-methylenedi-o-toluidine

838-88-0

4.6 250 mm5 m

22

biphenyl-4-ylamine
4-aminobiphenylxenylamine

92-67-1

3,3'-dichlorobenzidine
3,3'-dichlorobiphenyl-4,4'-ylenediamine

91-94-1

2.7 m

24

4-aminoazobenzene

60-09-3

2m

25

4,4'-methylene-bis-(2-chloro-aniline)
2,2'-dichloro-4.4-methylene-dianiline

16

23

26

o-aminoazotoluene
4-amino-2',3-dimethylazobenzene
4-o-tolylazo-o-toluidine

70 [1] Poroshell 120 SB-C18 EC-C18

Poroshell 120 EC-C18

150 mm

101-14-4

4, 4'-methylenedianiline
116-methoxy-m-toluidine (12)2,6-dimethylaniline

97-56-3

(13)2,4-dimethylaniline (14)3,3'-dimethoxybenzidine (15)

3,3'-dimethylbenzidine (16)

mAU

40

126

30

15

20

80%

10

26

10

6 8
1

24

-10

60%
(UV)
240 nm280 nm305 nm

12

0
4.5
8
9
10.5
12
14
15

14
29
29
50
65
90
100
14

20

19

13

14
15

22
23

17
16

10

18
7

4
5

2
mAU 0
40 280 nm
20

6 8

10

10

20

40% 150 mm
580 bar 600 bar UHPLC
Poroshell 120 EC-C18
3.0 mm 150 mm2.7 m 1290

2 2

25
22

15
11 1213 14

23

12

16
17 18

26

24

10

20 21

23
26

24
25

10

10

12

22

15 16

10

2. 240 nm280 nm305 nm

2.7 m 1.8 m

23
5

21
19

22

6 78

2
mAU 0
40 305 nm
20

11
14
1213
17
15 16

12

1. 3.0 mm 150 mm2.7 m Poroshell 120 EC-C18

240 nm 26
mAU
40 240 nm
20
3

26
B%

11

3.0 mm 4.6 mm

25

2.

21

18

24 25

12

26

3.0mm

1.

EN 14362-1-2003 -
1 (Textiles Methods for determination of certain aromatic amines
derived from azo colorants.Part 1:Detection of the use of
certain azo colorants accessible without extraction.)

2.

EN 14362-2-2003 -

150mm2.7 um Poroshell 120 EC-C18

15 26
1290
80%
60%

2
(Textiles.Methods for the determination of certain
aromatic amines derived from azo colorants.Part
2:Detection of the use of certain azo colorants
accessible by extracting the fibres.)
DAD1 A, Sig=240,4 Ref=off (AZOS DYES\...DYES-POROSHELL 120EC\DEF_LC 2010-08-1909-56-21\20100819000001.D)
DAD1 A, Sig=240,4 Ref=off (AZOS DYES\...DYES-POROSHELL 120EC\DEF_LC 2010-08-1909-56-21\20100819000002.D)
DAD1 A, Sig=240,4 Ref=off (AZOS DYES\...DYES-POROSHELL 120EC\DEF_LC 2010-08-1909-56-21\20100819000003.D)
DAD1 A, Sig=240,4 Ref=off (AZOS DYES\...DYES-POROSHELL 120EC\DEF_LC 2010-08-1909-56-21\20100819000004.D)
DAD1 A, Sig=240,4 Ref=off (AZOS DYES\...DYES-POROSHELL 120EC\DEF_LC 2010-08-1909-56-21\20100819000005.D)
DAD1 A, Sig=240,4 Ref=off (AZOS DYES\AZODYE-POROSHELLEC\20100819000002.D)

40

30

20

10

-10
0

10

12

3. 6 240 nm

www.agilent.com/chem

2011

2011 4 12
5990-7705CHTW