ABSTRACT

CORRELATION PRESENT BETWEEN THE POLAR BODY FOLLICLE

DIAMETER AND INCLUSION OOCYTE CYTOPLASM PRIMER ON
REPRODUCTIVE TECHNICAL PROGRAM IN VITRO FERTILIZATION
The controlled of ovarian stimulation on ART-IVF program has multifollicles effect with
wide variation of follicle diameter; but the oocyte quality is still questionable. Meanwhile, the
main indicators of oocyte quality are PB-I and CI. This study aims to know the correlation
between follicle diameter to the PB-1 positive and CI negative. The results of this study could be
use to determine follicle diameter needed to acquire good oocyte quality in order to increase
successful ART-IVF. An observational of non matching nested case control study is used,
conducted in Unit Bayi Tabung Graha Tunjung RSUP Sanglah and Prima Medika Hospital
Denpasar by year 2011-2012. The samples are follicles and oocytes during ovum pick up. The
follicle measurement are done by FER consultant using USG 2D Alokatype SSD 500
transvaginal during 36 hours after hCG administration. The cases group are follicle with
diameter 15 mm andthe controls group are follicle with diameter< 15 mm. The measurement of
PB-1 positive and SI negative are done by embriologist 4-6 hours after ovum pick up using the
electron microscope Nikon model. Furthermore, chi square test on SPSS-17 version for windows
is use to analyse OR and data are presented by table and narration. An amount of 70 follicles are
divided in two groups consists of 35 follicle diameter 15 mm as the cases and 35 follicle
diameter < 15 mm as the controls. The study found that the OR of PB-1 positive onfollicle 15
mm follicle are 5.46 times (ci 95% = 2.96-8.18, p= 0.01) compared with < 15 mm follicle
diameter. The OR of CI negative on 15 mm diameter follicle was 3.5 times (ci 95% = 2.377.86, p = 0.02) compare with < 15 mm follicle diameter. It also resulted the discriminant
coefficient of positive PB-1 and negative CI of follicle diameter 15 mm each are 0.97 and 0.76.
The Conclusions of an ART-IVF program are as follows.The probability of positive PB-1 and
negative CI of oocytes on follicle diameter 15 mm were 5.5 and 3.5 times over oocytes on
follicle diameter < 15 mm, eventually. Thus, in a ART-IVF program, the oocyte quality of larger
follicle is better than smaller on..
Keywords: follicle diameter, polar body-1, cytoplasmic inclusion.