Professional Documents
Culture Documents
Exercise 1
Exercise 1
Autoclaving / steam
under pressure
Bacillus stearothermophilus
- detect effectivitiy of
autoclave
- strip turns black
-
Boiling
-
Fractional/
tyndalization/
interminent
Inspissation
Action
Coagulation of
protein
Culture media
Coagulation of
protein
Kills only vegetative
not spores
Dental instruments.
Feeding bottles
Coagulation of
protein
Culture media that
cant withstand
autoclave
Germination spores
transformed into vegetative
cell then destroyed
- Coagulation of
protein
- Used with high
protein media
(which cant stand
autoclaving)
- Dorset egg medium,
Loffler serum,
Lowenstein jensen
HTST high temp short
time
Apparatus
Standard Condition
Autoclave
Inspissator
72C 15 s
LTH- low tem holding
5
Pasteurization
pasteurizer
60-63C 30 mins
Method
1
Hot air
Open flame
Action
Oxidation
Glassware, petri dishes
Spore strip: green then black
Bacillus subtilis var. Niger
Burning to ashes
Needles, loops, inoculating needle (red
hot)
Apparatus
Standard Condition
Oven
Bunsen
burner
Incenaration
Cremation
Burning to ashes
Waste products
Sputum cups, infected animals, wound
dressings
Cremate bodies with HIV
Incinerator