So you survived the MidtermCONGRATS! But, now its time to prepare for the final.

Yay! Moving on to defeating the next boss. (^~^)V

Worksheet 4
1. Enzymes increase the rate of a chemical reaction by decreasing the
_______________
a. How does the enzyme do this?

b. What does acetylcholinesterase do?

2. Serine Proteases (this will most certainly be on your final)

a. What is the catalytic triad? Give all 3 a.a.s and describe its
function in three steps.

i.
ii.
iii.
b. What is the oxyanion binding site? Why is it important?

c. What is the substrate specificity pocket? What does it recognize in

Chymotrypsin/Trypsin/Elastase/Subtilisin? What about the nonspecific binding site?

d. Mechanism of Action/Reaction steps: Describe the Acylation then Deacylation steps.

***You should know how to draw these steps as well!!!***

3. What happens when you put lipid bilayers into water?

a. What does amphipathic mean?

4. Identify the protein.

5. Write the product and label the N/C-terminus after its been cut by
chymotrypsin:
N-CHANYERS-C

6. Compare and contrast Chymotrypsin and Trypsin.