Identification of Staphylococcus aureus

1. Gram Staining
After gram staining and
being viewed by the use
of microscope, we saw a
round shape (cocci) and
violet in color (gram
positive)
Morphology: Gram
positive cocci in clusters

2. Catalase Test
Result: There was a formation
Staphylococcus specie because of
oxygen and water

3. Coagulase test

of bubbles(effervescence). It is a
there was a formation of free
because of the enzyme
catalase/peroxidase.

A. Slide test

Result: Both NSS and in rabbits plasma

gel-like consistency or formation of clot.
false negative result because of the
Staphylokinase enzyme that dissolves

have no
It is a
clot.

B. Tube test

Staphylococcus

Result: There is a formation of clot because of

the plasma proteins through the
presence of coagulase. Therefore this
specie is a Staphylococcus aureus.
R

4. Mannitol Salt Agar

Result : We identify this Staphylococcus specie

as a Staphylococcus aureus , because of the yellow
halo around colonies.

5. Novobiocin
Result : We determined this Staphylococcus specie as Staphylococcus
saprophyticus because the zone of inhibition appeared was < 16 (size: 2mm)
*if >16 (S. epidermidis)