R. oryzae (ATCC 9363) was used in this study.

It was sporulated on
streaked agar plates containing potato plates were stored at 4 C until
preparation of spore suspension. For each inoculation, spore concentration
in the suspension was determined by counting the spores on
hemocytometer. Then, R. oryzae was inoculated (102 spores/ml) into 50 ml
flasks containing 20 ml liquid medium composed of (all w/v) 2% glucose,
0.2% (NH4)2SO4, 0.065% KH2PO4, 0.025% MgSO4 7 H2O and 0.005%
ZnSO4 7 H2O. Multiple flasks were prepared and ran in parallel for each
experiment. Sampling was made at regular time intervals by taking three
of those flasks. Trehalose and cell dry weight analysis were carried out
with these flasks and average of three values were used.(9)

The inoculum was prepared by adding sterile water containing 0.05%

Tween 80 to a 7-day-old culture grown on potato dextrose agar plates at
30C.(7)

Potato pulp (dry matter 20.8%) was donated from a local plant that was
manufacturing starch from potato tubers. After the pulp was autoclaved at
121C for 5 min and cooled to room temperature, it was bagged in 10 g
amounts in zippered polyethylene (70 mm 40 mm, 0.04 mm thickness)
bags. Fungal spores that formed on potato dextrose agar for five days
were suspended in 0.01% Tween 80 and used to inoculate the sterilized
pulp with a final concentration of 105/g. A lump of the pulp was crumpled
daily and incubated at 25C for six days. The fermented pulp was mixed
with 30 ml of distilled water for 1 h and centrifuged to obtain the
supernatant.(3)
The organism used in this study, R. oryzae NRRL 395was maintained on
nutrient agar plates. It was incubated at 30 C. After growth and
sporulation, 10 ml of distilled water was aseptically added to each agar
plates which was then scraped to release the spores. This spore
suspension was centrifuged at 4000 rpm for 10 min, the spores were
washed and resuspended in 1ml distilled water.(5)