HOMEWORK

Ex 7.4 Aerobic growth of S. cerevisiae on ethanol is simply described by the

following overall reaction
C2H5OH + aO2 + bNH3

cCH1.704N0.149O0.408 + dCO2 + eH2O

a.) Determine the coefficients a, b, c and, d where RQ = 0.66

b.) Determine the biomass yield coefficient , Yx/s and oxygen yield coefficient Yx/O2
(gdw/gO2)
Soln a) Simple elemental balances, on C , H , O and , N yield the following equation
C:
H:
O:
N:

2(1)
2

= c(1) + d(1)
= c+d

____________(1)

5(1) + 1(1) + b(3) = c(1.704) + e(2)

6 + 3b
= 1.704c + 2e

____________(2)

1(1) + a(2)
1 + 2a

= c(0.408) + d(2) + e(1)

= 0.408c + 2d + e

____________(3)

b(1)
b

= c(0.149)
= 0.149c

____________(4)

The respiratory quotient (RQ)

0.66
d

=
=
=

CO2 / O2
d/a
0.66a
_____(5)

(5) (1)

c + 0.66a

_____(6)

1.704c + 2e
1.257c + 2e

_____(7)

(4) (2) 6 + 3(0.149c) =

6 =

(6) (7)

6 =
3.486 =

1.257(2 0.66a) + 2e
2e 0.830a _____(8)

1 + 2a =
0.184 =

0.408(2 0.66a) + 2(0.66a) + 1e

1e 0.949a _____(9)

0.368 =
3.118 =
a =

2e 1.898a
1.068a
2.919

(5),(6) (3)

(9) x 2
(8) (9)

a (5)

d
d

=
=

0.66(2.919)
1.926

d (1)

2
c

=
=

c + 1.926
0.074

c (4)

b
b

=
=

0.149(0.074)
0.011

b c (2)

6 + 3(0.011)
e

_____(10)

= 1.704(0.074) + 2e
= 2.953

C2H5OH + 2.919O2 + 0.011NH3

0.074CH1.704N0.149O0.408 + 1.926CO2 + 2.953H2O

C:

2
2
2

= c+d
= 0.074 + 1.926
= 2

H:

6 + 3b
6 + 3(0.011)
6.033

= 1.704c + 2e
= 1.704(0.074) + 2(2.953)
= 6.032

O:

1 + 2a
1+ 2(2.919)
6.838

= 0.408c + 2d + e
= 0.408(0.074) + 2(1.926) + 2.953
= 6.835

N:

b
0.011
0.011

=
=
=

0.149c
0.149(0.074)
0.011

RQ:

d
1.926
1.926

=
=
=

0.66a
0.66(2.919)
1.926

Soln

b) Molecular Weight Biomass , Substrate O2

C = 12
H=1
O = 16
N = 14
MW Biomass (x)
CH1.704N0.149O0.408
MW Substrate (s)
C2H5OH

MW O2

=
=

1(12) + 1.704(1) + 0.149(14) + 0.408(16)

22.318

=
=

2(12) + 5(1) + 1(16) + 1(1)

46

=
=

2(12)
32

 biomass ( Yx/s )
Yx/s

x
s

c Mwbiomass
1 MwC2 H 5OH

(0.074) 22.318
1 46

= 0.036 gdw cells /g ethanol

oxygen yield coefficient ( Yx/ O2 )
Yx/O2 =

x
O2

c Mwbiomass
a Mw(O2 )

(0.074) 22.318
2.919 46

= 0.012 gdw cells /gO2