PURITY CHECK OF PARAROSANILINE

Checking the purity of P-Rosaniline dye

1) Prepare Stock Pararosaniline Solution 0.1 gm of
Pararosaniline in distilled water. Stand for 48 hours then filter and
content in 50 ml volumetric flask and make up with distilled
water.
2) Prepare Solution 1 M Acetate acetic acid buffer stock solution
with pH of 4.79 by dissolving 13.61 gm of sodium Acetate
triydrate in distilled water in 10 ml volumetric flask. Add 5.7 ml of
glacial Acetic Acid and dilute to volume with distilled water.
3) Prepare 1 ml of the stock PRA solution to 100 ml volumetric flask
and dilute to volume with distilled water.
4) Transferred 5 ml aliquot of the diluted PRA solution from 2 into a 50
ml volumetric flask. Add 5 ml of 1 m acetate acetic acid buffer
solution from 1 and dilute the mixture to volume with distilled
water and let the mixture stand for 1 hour.
5) Take the absorbance of above solution at 540 nm.
nm
500
510
520
530
540
550
560

nm
nm
nm
nm
nm
nm
nm

Absorbance

6) The dye must have a maximum absorbance at a wavelength of 540

nm when assayed in a buffered solution of 0.1 M sodium acetate
acetic acid against a distilled water reference.
% PRA=Absorbance x 21.3/0.1
where,
21.3 = spectrophotometer having a spectral band with of less
than 15 nm and a path length of 1cm.
0.1 = weight of PRA for preparing stock solution