Scribd Logo
Upload

Welcome to Scribd!

  • 208 views

    ATCC Medium: 3 Nutrient Agar/Broth Agar Medium

    Uploaded by

    Josue Zuniga
    This document provides formulations for making nutrient agar and broth media from commercially available sources or from scratch. The nutrient agar medium contains 23g of nutrient agar and is autoclaved at 121°C. The nutrient broth medium contains 8g of nutrient broth and is also autoclaved at 121°C. Alternatively, the nutrient agar can be made from scratch by combining 3g beef extract, 5g peptone, 15g agar, and adjusting the final pH to 6.8-7 after autoclaving.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content

    You might also like

    ATCC Medium: 3 Nutrient Agar/Broth Agar Medium

    Uploaded by

    Josue Zuniga
    208 views1 page
    This document provides formulations for making nutrient agar and broth media from commercially available sources or from scratch. The nutrient agar medium contains 23g of nutrient agar and is autoclaved at 121°C. The nutrient broth medium contains 8g of nutrient broth and is also autoclaved at 121°C. Alternatively, the nutrient agar can be made from scratch by combining 3g beef extract, 5g peptone, 15g agar, and adjusting the final pH to 6.8-7 after autoclaving.

    Original Description:

    deneral

    Original Title

    ATCC Medium 3

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document provides formulations for making nutrient agar and broth media from commercially available sources or from scratch. The nutrient agar medium contains 23g of nutrient agar and is autoclaved at 121°C. The nutrient broth medium contains 8g of nutrient broth and is also autoclaved at 121°C. Alternatively, the nutrient agar can be made from scratch by combining 3g beef extract, 5g peptone, 15g agar, and adjusting the final pH to 6.8-7 after autoclaving.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    208 views1 page

    ATCC Medium: 3 Nutrient Agar/Broth Agar Medium

    Uploaded by

    Josue Zuniga
    This document provides formulations for making nutrient agar and broth media from commercially available sources or from scratch. The nutrient agar medium contains 23g of nutrient agar and is autoclaved at 121°C. The nutrient broth medium contains 8g of nutrient broth and is also autoclaved at 121°C. Alternatively, the nutrient agar can be made from scratch by combining 3g beef extract, 5g peptone, 15g agar, and adjusting the final pH to 6.8-7 after autoclaving.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    ATCC Medium: 3 Nutrient Agar/Broth

    Agar Medium
    Nutrient Agar (BD 213000)..23 g
    DI Water.1000 ml
    Autoclave at 121C.
    Broth Medium
    Nutrient Broth (BD cat 234000).8.0 g
    DI Water...1000 ml
    Autoclave at 121C.
    *This product is commercially available from BD.
    To make medium from scratch, follow formulation below:
    Nutrient Agar Composition
    Beef Extract....3.0 g
    Peptone...5.0 g
    Agar.15.0 g
    Final pH 6.8 +/- 0.2.
    *Omit agar for broth medium.

    You might also like