Anti-inflammatory effect of

Glucocorticoid

Bo Hong
Department of Pharmacology

Purpose:

Observe the anti-inflammatory effect of

Glucocorticoid.

Grasp

the

method

to

establish

inflammatory model of the ear of mice.

the

Principle
The anti-inflammatory effect of Glucocorticoid is very
strong, it is effective on all kinds of inflammation and
every stage.
Dimethylbenzene and other inflammatory substances
was wiped on the ear of animals, it will damage part
cells, accelerate the release of histamine, bradykinin
and other inflammatory substances, lead to the acute
edema in ear.

Ointment

The ear will become incrassation because of the

increasing permeability in blood capillary.
We can judge the anti-inflammatory effect of
drugs.

[Materials]
1. Animals: Mouse, weight of 25-30 g, male.
2. Drugs: 0.5% hydrocortisone injection,
dimethylbenzene, normal saline.
3. Instrument: syringe (1 mL2), analytical balance,
mouse puncher (diameter of 8 mm).

[Method]
Two male mice every group, weight of 25-30 g,
weigh and mark. Dimethylbenzene of 0.05-0.1 mL
was wiped in the left ear (front and back) of the
two mice.
30 mins later, 0.5% hydrocortisone injection was
intraperitoneally injected into one mouse (0.1
mL/10 g), the same dose of normal saline was
intraperitoneally injected into the other mouse.

Method
The mice will be dislocated executed after
administration for 1.5 hours. We get the ear piece
from right and left ears in the same place of every
mouse using the diameter of punch, then weigh
them using the table balance.

The ear piece weight difference between left

ear and right ear can evaluate the swollen
degree. Summarize the experimental data
and record in the table.

[Results]
Anti-inflammatory effect of Glucocorticoid (ear
piece of mice)
Group

hydrocortisone
Normal saline

Amount
Average weight
of
Left ear Right ear
Swollen
mice
degree

[Notes]

The mice auricle inflammatory model could

also be established using 2% croton oil and
70% alcohol solution.