GRE

)n :(= ) m( ) M(.

- . Ei
Ei . .EV

.
<----- .

: .

: .
- .
-

PH

: .
. .
: .
. .

-E0 - ) ,(E0
) (

GIBBS FREE ENERGY

H- ENTALPHY
-S- ENTROPY . .
-T.

EQUILIBRIUM STEADY
STATE

GRE

K=ln2\t0.5 -0
-0

:
:
* )2 ([[ * I= 1\2
: MgCl2 I=0.5 [0.5
* 22 + 0.5* (-1)2 * 2

0.5M

 : ,
. ) ,(MgCl2
.(NaCl) 2

 GRE

:ISOELECTRIC POINT
) H( Pi=(pK1+pK2)\2
)(GLUTAMIC ACID, ASPARTIC ACID pKr)\2-
+Pi=(pK1
) (LYSINE, ARGENINE, HISTIDINE
Pi=(pKr+pK2) \ 2

ES .P+ E :
) ES= ET (.

 V0

A=EXCXL

BEER LAMBART LAW

A
L
C mol L^-1
E L mol^-1 cm^-1
) A( .
) 280nm (

)(COMPETITIVE INHIBITION

-NON COMPETITIVE
. ,VMAX .KM

(UNCOMPETITIVE)

MI
X
E
D
I
N
H
I
B
I
T
I
O
N

ALLOSTERIC ENZYMES