2013-1205

Chondrocytes
Arvind Manikantan Padma
Transplantation and Regenerative
Medicine
Sahlgrenska Science park

Introduction
Make up cellular matrix
Mature cells are round and form
clusters
Perform number of functions
Progenitor arises from bone marrow
Support and repair cell matrix
Composition differs with type of
cartilage
Present in different places in the

Overview of paper
External ear reconstruction
Tough implantation due to lack of
vasculature
Cells from rabbit taken and expanded
Successful reconstruction of cartilage
Including cartilage and
perichondrium

Markers

CD 44
CD 151
Aggrecan
Cathepsin B
CEP -68
Collagen 2
Collagen 9
Collagen X
Matrilin 1
Osteonecin
Sox9
YKL 39 and 40

BD Biosciences

Millipore

CD markers
CD 44
-Receptor for hyaluronic acid
- Controlled by posttranslational
modifications
- Wide variety of cell functions
CD 151
- Protein is from transmembrane 4
family
- 4 hydrophobic domains
- Mediate cell processes

Collagen
Collagen 2
- Makes up 50% of cartilage
- It forms fibrils
Collagen 9
- Component of hyaline cartilage
- Found with type 2 collagen
Collagen X
- Short collagen chain
- Expressed by hypertrophic chondrocytes
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Other proteins
Aggrecan
- Cartilage specific proteoglycan core protein
- Integral part of ECM of cartilage
- Has 3 globular domains
CEP-68
- Harbors N terminal leader peptide
- Complements Collages 2
Matrilin 1
- vWB factor A containing protein family
- Involved in filamentous networks in ECM
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Other proteins
Sox 9
- Acts during chondrocyte
differentiation
- It also plays a role in male sexual
development
YKL 39 and 40
- Glycoprotein 40 kDA in size
- 3 N terminal amino acids.
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Conclusion
The method used in the paper was by
using mouse monoclonal antibodies
against Col 2 and biotinylated anti mouse
secondary antibodies
For collagen X, rabbit monoclonal
antibodies were used and anti rabbit
secondary antibodies were used
For elastin, mouse monoclonal antibodies
and anti mouse secondary antibodies were
used
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References

Renata Giardini Rosa et al; 2013; Development of ScaffoldFree Elastic Cartilaginous Constructs with Structural
Similarities to Auricular Cartilage
http://www.wisegeek.org/what-are-chondrocytes.htm
http://www.bdbiosciences.com/nvCategory.jsp?
action=SELECT&form=formTree_catBean&item=746206
http://www.millipore.com/techpublications/tech2/chondrocyt
e
http://www.rndsystems.com/molecule_group.aspx?
g=820&r=7
http://www.ncbi.nlm.nih.gov/pubmed/9171829
Tissue engineering of cartilage by Mats Brittberg and
Anders Lindahl
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Thank You!

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