Identifikasi Kadar Antioksidan Dalam Darah Dengan Menggunaka

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n\paperw11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg1}{\bkmkend Pg1}\par\pard
\ql \li10089\sb0\sl-276\slmult0 \par\pard\ql\li10089\sb155\sl-276\slmult0 \up0 \
expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 1 \par\pard\ql \li2275\sb0\s
l-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb180\sl276\slmult0 \up0 \expndtw-1\charscalex100 \ul0\nosupersub\cf2\f3\fs24 A.\ul0\nos
upersub\cf3\f4\fs24 \ul0\nosupersub\cf2\f3\fs24 JUDUL PROGRAM \par\pard\qj \li2
275\ri1501\sb1\sl-280\slmult0\fi851 \up0 \expndtw0\charscalex100 \ul0\nosupersub
\cf1\f2\fs24 Identifikasi Kadar Antioksidan Dalam Darah dengan Menggunakan

\up0 \expndtw-3\charscalex100 Metode Spektrofotometri. \par\pard\ql \li2275\sb0
\sl-276\slmult0 \par\pard\ql\li2275\sb8\sl-276\slmult0 \up0 \expndtw0\charscalex
100 \ul0\nosupersub\cf2\f3\fs24 B.\ul0\nosupersub\cf3\f4\fs24 \ul0\nosupersub\c
f2\f3\fs24 LATAR BELAKANG \par\pard\qj \li2275\ri1499\sb0\sl-277\slmult0\fi851 \
up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs24 Salah satu zat yang san
gat penting untuk menjaga kesehatan tubuh adalah \up0 \expndtw0\charscalex104 an
tioksidan. Antioksidan adalah molekul yang menghambat dan menetrealisir \up0 \ex
pndtw-2\charscalex100 radikal bebas. Zat ini sangat bermanfaat untuk mencegah te
rjadinya penyakit yang \up0 \expndtw0\charscalex108 ditimbulkan oleh radikal beb
as. penyakit yang sering di hubungkan dengan \up0 \expndtw-1\charscalex100 radik
al bebas diantaranya penyakit kanker, serangan jantung, serta penuaan dini. \u
p0 \expndtw0\charscalex107 Beberapa tahun terakhir orang yang terserang penyakit
kanker dan serangan \up0 \expndtw0\charscalex103 jantung semakin banyak. Hal in
i dapat disebabkan karena tubuh manusia yang \up0 \expndtw0\charscalex104 terlal
u banyak menerima paparan radikal bebas terutama dari lingkungan \up0 \e
xpndtw-3\charscalex100 sekitarnya yang banyak mengandung racun dan polusi. \par
\pard\qj \li2275\ri1501\sb0\sl-277\slmult0\fi851 \up0 \expndtw0\charscalex102 pe
nyakit yang disebabkan oleh radikal bebas bersifat kronis, yaitu \line \
up0 \expndtw0\charscalex105 dibutuhkan waktu bertahun-tahun untuk penyakit t
ersebut menjadi nyata. \line \up0 \expndtw0\charscalex109 Radikal bebas yang
mengambil elektron dari sel tubuh manusia dapat \line \up0 \expndtw0\char
scalex103 menyebabkan perubahan struktur DNA sehingga timbulah sel-sel mutan.
Bila \line \up0 \expndtw0\charscalex100 perubahan DNA ini terjadi bertahun-tahun
, maka dapat menjadi penyakit kanker. \line \up0 \expndtw0\charscalex107 Lipid y
ang seharusnya menjaga kulit agar tetap segar berubah menjadi lipid \line \up0 \
expndtw0\charscalex100 peroksida karena bereaksi dengan radikal bebas sehingga m
empercepat penuaan. \line \up0 \expndtw0\charscalex104 Selain itu, oksigen re
aktif dapat meningkatkan kadar LDL \up0 \expndtw0\charscalex103 (\ul0\nosupe
rsub\cf4\f5\fs24 low density \par\pard\qj \li2275\ri1499\sb0\sl-270\slmult0 \up
0 \expndtw0\charscalex100 lipoprotein\ul0\nosupersub\cf1\f2\fs24 ) yang kemudi
an menjadi penyebab penimbunan kolesterol pada \up0 \expndtw-1\charscalex10
0 dinding pembuluh darah. Akibatnya timbullah \ul0\nosupersub\cf4\f5\fs24 arther
osklerosis\ul0\nosupersub\cf1\f2\fs24 atau lebih dikenal \up0 \expndtw-3\charsc
alex100 dengan penyakit jantung koroner. \par\pard\qj \li2275\ri1500\sb0\sl-277\
slmult0\fi851 \up0 \expndtw0\charscalex101 Mengingat sangat pentingnya antiok
sidan untuk menangkal bahaya \up0 \expndtw-1\charscalex100 radikal bebas maka
identifikasi kadar antioksidan dalam tubuh sangat di perlukan. \up0 \expndtw0\c
harscalex104 Dengan memeriksa status antioksidan maka dapat diketahui informasi
tentang \up0 \expndtw-1\charscalex100 kapasitas status antioksidan seseorang. Se
rta untuk menilai daya tahan tubuh atau \up0 \expndtw0\charscalex100 paerlindung
an tubuh terhadap serangan radikal bebas. Untuk selanjutnya dapat di \up0 \expnd
tw0\charscalex103 tentukan perlu tidaknya terapi atau suplemen antioksidan, dan
dapat dilakukan \up0 \expndtw0\charscalex100 upaya untuk mencegah timbulnya peny
akit yang berkaitan dengan radikal bebas, \up0 \expndtw-3\charscalex100 serta me
mantau terapi / pemberian suplemen antioksidan. \par\pard\qj \li2275\ri1501\sb0\
sl-260\slmult0\fi851 \up0 \expndtw0\charscalex101 Salah satu pemeriksaan laborat
orium untuk menilai sistem antioksidan \line \up0 \expndtw0\charscalex106 tubuh
yaitu Status Antioksidan Total \up0 \expndtw0\charscalex104 (SAT), merupakan pem
eriksaan untuk \par\pard\qj \li2275\ri1498\sb5\sl-277\slmult0 \up0 \expndtw0\cha
rscalex103 mengukur kapasitas dan aktivitas total antioksidan yang terdapat dala
m tubuh. \line \up0 \expndtw0\charscalex103 Dalam pengertian klinis yang sering
dianggap sebagai antioksidan total adalah \line \up0 \expndtw0\charscalex106 ant
ioksidan yang mempunyai berat molekul besar (antioksidan primer) serta \line \up
0 \expndtw0\charscalex106 berfungsi mencegah terbentuknya radikal bebas, dalam h
al ini : SOD (\ul0\nosupersub\cf4\f5\fs24 Super \line \up0 \expndtw0\charscalex1
07 oksida dismutase\ul0\nosupersub\cf1\f2\fs24 ) dan \ul0\nosupersub\cf4\f5\fs24
Glutation peroksidase\ul0\nosupersub\cf1\f2\fs24 (GPx). Akan tetapi selama in
i \line \up0 \expndtw0\charscalex100 pengukuran kadar antioksidan dalam tubuh s
angat jarang. Biasanya pengukuran \line \up0 \expndtw0\charscalex102 dilakukan d
engan cara kimia serta kalaupun ada alat otomatis, harganya masih \line \up0 \e
xpndtw-3\charscalex100 relatif sangat mahal. \par\pard\qj \li2275\ri1500\sb9\sl270\slmult0\fi851 \up0 \expndtw-3\charscalex100 Untuk mengetahui status antioksi
dan total(SAT) dalam tubuh maka dapat \line \up0 \expndtw0\charscalex104 diukur
kadar SOD (\ul0\nosupersub\cf4\f5\fs24 Super oksida dismutase\ul0\nosupersub\cf1
\f2\fs24 ) dan \ul0\nosupersub\cf4\f5\fs24 Glutation peroksidase\ul0\nosupersub\
cf1\f2\fs24 (GPx) \line \up0 \expndtw0\charscalex100 dalam darah. Oleh karena i
tu identifikasi kadar antioksidan dalam darah dengan \par\pard\sect\sectd\fs24\p
aperw11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg2}{\bkmkend Pg2}\par\pard\ql
\li10089\sb0\sl-276\slmult0 \par\pard\ql\li10089\sb155\sl-276\slmult0 \up0 \exp
ndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 2 \par\pard\qj \li2275\sb0\sl-2
75\slmult0 \par\pard\qj\li2275\sb0\sl-275\slmult0 \par\pard\qj\li2275\sb0\sl-275
\slmult0 \par\pard\qj\li2275\sb0\sl-275\slmult0 \par\pard\qj\li2275\ri1495\sb185
\sl-275\slmult0 \up0 \expndtw0\charscalex102 menggunakan metode spektrofotomeri
merupakan salah satu upaya identifikasi \up0 \expndtw0\charscalex100 kadar antio
ksidan dalam darah. Secara teoritis kalau kadar ini dalam darah dapat \up0 \expn
dtw0\charscalex100 ditentukan maka dapat diprediksi adanya bahaya radikal bebas.
Selain itu cara ini \up0 \expndtw0\charscalex100 dapat memberikan solusi identi
fikasi kadar antioksidan dalam darah yang efektif \up0 \expndtw-3\charscalex100
dan efesien dari segi penggunaan maupun segi biaya. \par\pard\ql \li2275\sb0\sl276\slmult0 \par\pard\ql\li2275\sb9\sl-276\slmult0 \up0 \expndtw-1\charscalex100
\ul0\nosupersub\cf2\f3\fs24 C.\ul0\nosupersub\cf3\f4\fs24 \ul0\nosupersub\cf2\
f3\fs24 RUMUSAN MASALAH \par\pard\qj \li2275\ri1500\sb4\sl-276\slmult0\fi708 \up
0 \expndtw0\charscalex101 \ul0\nosupersub\cf1\f2\fs24 Pada perangkat pendeteksi
kadar antioksidan dalam darah ini digunakan \up0 \expndtw0\charscalex100 sensor
cahaya untuk mendeteksi gelombang cahaya yang dibiaskan oleh sampel \up0 \expn
dtw0\charscalex104 darah. Keluaran dari sensor berupa sinyal listrik akan di ter
uskan ke komputer \up0 \expndtw0\charscalex102 untuk diproses sekaligus hasilnya
akan ditampilkan pada layar komputer. Hasil \up0 \expndtw0\charscalex103 yang d
idapat akan dianalisis untuk mencari hubungan antara kadar antioksidan \up0 \exp
ndtw-3\charscalex100 dalam darah dengan tegangan keluaran sensor. \par\pard\ql \
li2342\sb0\sl-276\slmult0 \par\pard\ql\li2342\sb8\sl-276\slmult0 \up0 \expndtw-1
\charscalex100 \ul0\nosupersub\cf2\f3\fs24 D.\ul0\nosupersub\cf3\f4\fs24 \ul0\n
osupersub\cf2\f3\fs24 TUJUAN PENELITIAN \par\pard\qj \li2275\ri1498\sb0\sl-275\s
lmult0\fi768 \up0 \expndtw0\charscalex107 \ul0\nosupersub\cf1\f2\fs24 Tujuan pen
elitian ini adalah mengidentifikasi atau mengukur kadar \up0 \expndtw-1\charsca
lex100 antioksidan dalam darah dengan metode spektrofotomeri dan diproses kompu
ter. \up0 \expndtw0\charscalex103 Identifikasi atau pengukur tersebut akan
dianalisis hubungan antara kadar \up0 \expndtw0\charscalex102 antioksidan da
lam darah dengan tegangan keluaran sensor dan diharapkan \up0 \expndtw-3\
charscalex100 mempunyai keakuratan yang dapat terpercaya. \par\pard\ql \li2275\s
b270\sl-276\slmult0 \up0 \expndtw-1\charscalex100 \ul0\nosupersub\cf2\f3\fs24 E.
\ul0\nosupersub\cf3\f4\fs24 \ul0\nosupersub\cf2\f3\fs24 LUARAN YANG DIHARAPKAN
\par\pard\qj \li2275\ri1497\sb4\sl-276\slmult0\fi851 \up0 \expndtw0\charscalex10
3 \ul0\nosupersub\cf1\f2\fs24 Dari penelitian ini diharapkan menghasikan metode
identifikasi kadar \line \up0 \expndtw0\charscalex104 antioksidan dalam darah
kemudian dapat diaplikasikan dalam bentuk alat \line \up0 \expndtw0\charsc
alex103 pengukur kadar antioksidan dalam darah sehingga dapat memenuhi kebutuhan
\line \up0 \expndtw-1\charscalex100 dan ketersediaan alat yang mempunyai keungg
ulan dalam hal efisiensi dan biaya. \line \up0 \expndtw0\charscalex103 Selain it
u alat ini di harapkan dapat memberikan kontribusi dalam peningkatan \line \up0
\expndtw-3\charscalex100 kesehatan masyarakat. \par\pard\ql \li2275\sb0\sl-276\s
lmult0 \par\pard\ql\li2275\sb8\sl-276\slmult0 \up0 \expndtw-2\charscalex100 \ul0
\nosupersub\cf2\f3\fs24 F.\ul0\nosupersub\cf3\f4\fs24 \ul0\nosupersub\cf2\f3\fs
24 KEGUNAAN \par\pard\qj \li2275\ri1497\sb0\sl-280\slmult0\fi851 \up0 \expndtw0
\charscalex103 \ul0\nosupersub\cf1\f2\fs24 Penelitian ini bermanfaat untuk memu
dahkan cara identifikasi kadar \up0 \expndtw0\charscalex106 antioksidan dalam d
arah dengan keakuratan yang cukup baik sehingga dapat \up0 \expndtw0\charscalex1
03 diaplikasikan dalam bidang medis sebagai salah satu alternatif
dalam \up0 \expndtw-3\charscalex100 pengidentifikasi kadar antioksidan dalam d
arah. \par\pard\ql \li2275\sb245\sl-276\slmult0 \up0 \expndtw-1\charscalex100 \u

l0\nosupersub\cf2\f3\fs24 G.\ul0\nosupersub\cf3\f4\fs24 \ul0\nosupersub\cf2\f3\
fs24 TINJAUAN PUSTAKA \par\pard\ql \li2275\sb4\sl-276\slmult0 \up0 \expndtw-3\ch
arscalex100 G.1 Antioksidan Dan Radikal Bebas \par\pard\qj \li2275\ri1499\sb4\s
l-276\slmult0\fi851 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs24 An
tioksidan adalah molekul yang menghambat dan menetrealisir radikal \up0 \expndtw
0\charscalex105 bebas. Zat ini sangat bermanfaat untuk mencegah terjadinya penya
kit yang di \up0 \expndtw0\charscalex100 timbulkan oleh radikal bebas. Cara ker
ja senyawa antioksidan adalah bereaksi \up0 \expndtw0\charscalex100 dengan ra
dikal bebas reaktif membentuk radikal bebas tak reaktif yang relatif \up0 \e
xpndtw0\charscalex100 stabil. Antioksidan menstabilkan radikal bebas dengan me
lengkapi kekurangan \up0 \expndtw-2\charscalex100 elektron yang dimiliki radi
kal bebas, dan menghambat terjadinya reaksi berantai \up0 \expndtw-3\charscalex
100 dari pembentukan radikal bebas. \par\pard\qj \li2275\ri1506\sb0\sl-280\slmul
t0\fi851 \up0 \expndtw0\charscalex107 Radikal bebas merupakan suatu atom, moleku
l, atau senyawa yang \line \up0 \expndtw0\charscalex108 mengandung satu atau leb
ih elektron yang tidak berpasangan dalam orbital \par\pard\sect\sectd\fs24\paper
w11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg3}{\bkmkend Pg3}\par\pard\ql \li
10089\sb0\sl-276\slmult0 \par\pard\ql\li10089\sb155\sl-276\slmult0 \up0 \expndtw
-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 3 \par\pard\qj \li2275\sb0\sl-276\s
lmult0 \par\pard\qj\li2275\sb0\sl-276\slmult0 \par\pard\qj\li2275\sb0\sl-276\slm
ult0 \par\pard\qj\li2275\sb0\sl-276\slmult0 \par\pard\qj\li2275\ri1499\sb180\sl276\slmult0 \up0 \expndtw-2\charscalex100 luarnya. Karena kehilangan pasangannya
itu, sifatnya menjadi tidak stabil, reaktif. \up0 \expndtw0\charscalex100 dan r
adikal. Akibatnya, radikal bebas selalu berusaha mencari pasangan elektron \up0
\expndtw0\charscalex104 yakni merebut elektron dari molekul lain yang di sebut r
eaksi oksidasi. Reaksi \up0 \expndtw0\charscalex106 oksidasi dapat menghasilkan
radikal bebas baru dan memicu reaksi berantai \up0 \expndtw0\charscalex100 sehi
ngga jumlah radikal bebas semakin banyak. Reaksi oksidasi berujung pada \up0
\expndtw-3\charscalex100 timbulnya suatu penyakit. \par\pard\qj \li2275\ri1500\s
b4\sl-277\slmult0\fi851 \up0 \expndtw0\charscalex103 Efek oksidatif radikal b
ebas dapat menyebabkan peradangan dan \line \up0 \expndtw0\charscalex107 pen
uaan dini. Lipid yang seharusnya menjaga kulit agar tetap segar berubah \line \u
p0 \expndtw0\charscalex100 menjadi \ul0\nosupersub\cf4\f5\fs24 lipid peroksi
da\ul0\nosupersub\cf1\f2\fs24
karena bereaksi dengan radikal bebas
sehingga \line \up0 \expndtw0\charscalex100 mempercepat penuaan. Kanker pun dise
babkan oleh oksigen reaktif yang intinya \line \up0 \expndtw-1\charscalex100 mem
acu zat karsinogenik, sebagai faktor utama kanker. Selain itu, oksigen reaktif \
line \up0 \expndtw-1\charscalex100 dapat meningkatkan kadar LDL (\ul0\nosupersub
\cf4\f5\fs24 low density lipoprotein\ul0\nosupersub\cf1\f2\fs24 ) yang kemudian
menjadi \line \up0 \expndtw0\charscalex102 penyebab penimbunan kolesterol pad
a dinding pembuluh darah. Akibatnya \line \up0 \expndtw-2\charscalex100 timb
ullah \ul0\nosupersub\cf4\f5\fs24 artherosklerosis\ul0\nosupersub\cf1\f2\fs24 a
tau lebih dikenal dengan penyakit jantung koroner. \par\pard\qj \li2275\ri1500\s
b0\sl-280\slmult0\fi851 \up0 \expndtw-1\charscalex100 Antioksidan ini terdiri da
ri berbagai macam komponen baik\ul0\nosupersub\cf4\f5\fs24 intraselular \line \
up0 \expndtw0\charscalex100 \ul0\nosupersub\cf1\f2\fs24 (di dalam sel/\ul0\nosup
ersub\cf4\f5\fs24 endogen\ul0\nosupersub\cf1\f2\fs24 ), maupun \ul0\nosupersub\c
f4\f5\fs24 ekstraselular\ul0\nosupersub\cf1\f2\fs24 (di luar sel/\ul0\nosupersu
b\cf4\f5\fs24 eksogen\ul0\nosupersub\cf1\f2\fs24 ). Antioksidan \line \up0 \expn
dtw0\charscalex104 terbagi menjadi dua yaitu antioksidan enzim dan vitamin. Ant
ioksidan enzim \line \up0 \expndtw0\charscalex100 meliputi superoksida dismuta
se \up0 \expndtw0\charscalex102 (SOD), katalase dan glutation peroksidase \p
ar\pard\qj \li2275\ri1505\sb0\sl-270\slmult0 \up0 \expndtw0\charscalex100 (GSH.P
rx). Antioksidan vitamin lebih populer sebagai antioksidan dibandingkan \up0 \ex
pndtw0\charscalex103 enzim. Antioksidan vitamin mencakup alfa tokoferol (vitamin
E), beta karoten \up0 \expndtw-3\charscalex100 dan asam askorbat (vitamin C). \
par\pard\ql \li3127\sb2\sl-268\slmult0 \up0 \expndtw-3\charscalex100 Terdapat 3
kelompok antioksidan dalam tubuh: \par\pard\ql \li2342\ri1504\sb11\sl-270\slmult
0\tx2702\tx2702 \up0 \expndtw0\charscalex102 1.\ul0\nosupersub\cf6\f7\fs24 \ul0
\nosupersub\cf7\f8\fs24 Primary Antioxidants\ul0\nosupersub\cf2\f3\fs24 \ul0\n

osupersub\cf1\f2\fs24 (Antioksida Primer). Bekerja dengan cara mencegah \line\ta
b \up0 \expndtw-2\charscalex100 pembentukan radikal bebas baru. Contoh: SOD (Sup
eroxide Dismutase), GPx \line\tab \up0 \expndtw0\charscalex102 (Glutathione Per
oxidase), dan Protein Pengikat Metal \up0 \expndtw0\charscalex101 (Metal Bi
nding \par\pard\ql \li2702\sb6\sl-276\slmult0 \up0 \expndtw-3\charscalex100 Prot
ein) seperti Ferritin atau Ceruloplasmin. \par\pard\li2342\sb14\sl-276\slmult0\f
i0\tx5350 \up0 \expndtw-1\charscalex100 2.\ul0\nosupersub\cf6\f7\fs24 \ul0\nosu
persub\cf7\f8\fs24 Secondary Antioxidants\tab \up0 \expndtw-1\charscalex100 \u
l0\nosupersub\cf1\f2\fs24 (Antioksidan Sekunder). Berfungsi menangkap\par\par
d\li2342\sb0\sl-276\slmult0\fi360 \up0 \expndtw0\charscalex100 senyawa radikal s
erta mencegah terjadinya reaksi berantai. Contoh: Vitamin\par\pard\li2342\sb0\sl
-276\slmult0\fi360\tx3052\tx6310 \up0 \expndtw-1\charscalex100 E\tab \up0 \expnd
tw-1\charscalex100 (alpha-tokoferol), Vitamin C\tab \up0 \expndtw-1\charscal
ex100 (askorbat), beta-karoten, Asam urat,\par\pard\li2342\sb0\sl-276\slmu
lt0\fi360 \up0 \expndtw-1\charscalex100 Flavonoid, Bilirubin dan Albumin.\par\pa
rd\ql \li2342\sb1\sl-259\slmult0\tx5345 \up0 \expndtw0\charscalex101 3.\ul0\nosu
persub\cf6\f7\fs24 \ul0\nosupersub\cf7\f8\fs24 Tertiary Antiokxidants \tab \
up0 \expndtw0\charscalex101 \ul0\nosupersub\cf1\f2\fs24 (Antioksidan Tersier).
Antioksidan jenis ini \par\pard\qj \li2702\ri1501\sb4\sl-280\slmult0 \up0
\expndtw0\charscalex100 memperbaiki kerusakan sel-sel dan jaringan yang disebab
kan radikal bebas. \line \up0 \expndtw0\charscalex105 Contoh: enzim-enzim yang m
emperbaiki DNA dan metionim sulfoksida \line \up0 \expndtw-3\charscalex100 reduk
tase. \par\pard\qj \li2275\ri1500\sb0\sl-280\slmult0\fi851 \up0 \expndtw-3\chars
calex100 Keseimbangan antara antioksidan dan radikal bebas menjadi kunci utama \
line \up0 \expndtw-2\charscalex100 pencegahan stres oksidatif dan penyakit-penya
kit kronis yang dihasilkannya. Stres \line \up0 \expndtw0\charscalex103 oksidati
f adalah ketidak seimbangan antara radikal bebas dan antioksidan yang \line \up0
\expndtw0\charscalex103 dipicu oleh dua kondisi umum; kurangnya antioksidan dan
kelebihan produksi \line \up0 \expndtw-3\charscalex100 radikal bebas. \par\pard
\sect\sectd\fs24\paperw11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg4}{\bkmken
d Pg4}\par\pard\ql \li10089\sb0\sl-276\slmult0 \par\pard\ql\li10089\sb155\sl-276
\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 4 \par\pard\q
l \li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql
\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\l
i2275\sb180\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\
fs24 G.2 Darah \par\pard\ql \li2275\sb4\sl-276\slmult0 \up0 \expndtw-3\charscale
x100 G.2.1 Konsep dasar tentang darah \par\pard\qj \li2275\ri1501\sb9\sl-270\slm
ult0\fi708 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs24 Darah manus
ia berwarna merah, antara merah terang apabila kaya oksigen \line \up0 \expndtw0
\charscalex104 sampai merah tua apabila kekurangan oksigen. Warna merah
pada darah \line \up0 \expndtw0\charscalex100 disebabkan oleh {\field{\*\fldi
nst {HYPERLINK "http://id.wikipedia.org/wiki/Hemoglobin" }}{\fldrslt {\ul0\nosup
ersub\cf1\f2\fs24 hemoglobin, protein pernapasan}}} \up0 \expndtw0\charscalex1
00 \ul0\nosupersub\cf1\f2\fs24 (\ul0\nosupersub\cf4\f5\fs24 respiratory protein
\ul0\nosupersub\cf1\f2\fs24 ) yang \par\pard\qj \li2275\ri1504\sb2\sl-280\slmul
t0 \up0 \expndtw0\charscalex103 mengandung {\field{\*\fldinst {HYPERLINK "http:
//id.wikipedia.org/wiki/Besi" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 besi}}}\u
l0\nosupersub\cf1\f2\fs24 dalam bentuk {\field{\*\fldinst {HYPERLINK "http:/
/id.wikipedia.org/wiki/Heme" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 heme,}}}\u
l0\nosupersub\cf1\f2\fs24 yang merupakan tempat terikatnya \line \up0 \expn
dtw-3\charscalex100 molekul-molekul oksigen. \par\pard\qj \li2275\ri1499\sb5\sl275\slmult0\fi708 \up0 \expndtw0\charscalex102 Darah terdiri daripada beberapa j
enis korpuskula yang membentuk 45% \line \up0 \expndtw0\charscalex100 bagian dar
i darah, angka ini dinyatakan dalam nilai {\field{\*\fldinst {HYPERLINK "http://
id.wikipedia.org/w/index.php?title=Hermatokrit&action=edit&redlink=1" }}{\fldrsl
t {\ul0\nosupersub\cf1\f2\fs24 hermatokrit}}}\ul0\nosupersub\cf1\f2\fs24 atau v
olume sel \line \up0 \expndtw0\charscalex105 darah merah yang dipadatkan yang be
rkisar antara 40 % sampai 47%. Bagian \line \up0 \expndtw-1\charscalex100 sekita
r 55% yang lain berupa cairan kekuningan yang membentuk medium cairan \line \up0
\expndtw-3\charscalex100 darah yang disebut {\field{\*\fldinst {HYPERLINK "http

://id.wikipedia.org/wiki/Plasma_darah" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24
plasma darah.}}} \par\pard\ql \li2983\sb5\sl-276\slmult0 \up0 \expndtw-3\charsca
lex100 \ul0\nosupersub\cf1\f2\fs24 Korpuskula darah terdiri dari: \par\pard\ql \
li2342\sb24\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \u-3913?\ul0\nosupersub
\cf6\f7\fs24 \ul0\nosupersub\cf4\f5\fs24 {\field{\*\fldinst {HYPERLINK "http:
//id.wikipedia.org/wiki/Sel_darah_merah" }}{\fldrslt {\ul0\nosupersub\cf4\f5\fs2
4 Sel darah merah}}}\ul0\nosupersub\cf4\f5\fs24 atau {\field{\*\fldinst {HYPERL
INK "http://id.wikipedia.org/wiki/Eritrosit" }}{\fldrslt {\ul0\nosupersub\cf4\f5
\fs24 eritrosit}}}\ul0\nosupersub\cf1\f2\fs24 (sekitar 99%). \par\pard\qj \li27
02\ri1501\sb0\sl-280\slmult0\fi707 \up0 \expndtw0\charscalex104 Eritrosit tidak
mempunyai {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/Nukleus_se
l" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 nukleus sel}}}\ul0\nosupersub\cf1\f2
\fs24 ataupun {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/w/index.ph
p?title=Organela&action=edit&redlink=1" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24
organela,}}}\ul0\nosupersub\cf1\f2\fs24 dan tidak \line \up0 \expndtw-1\charsc
alex100 dianggap sebagai {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/
wiki/Sel_biologi" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 sel dari segi biologi
.}}}\ul0\nosupersub\cf1\f2\fs24 Eritrosit mengandung hemoglobin dan \line \up0
\expndtw-2\charscalex100 mengedarkan oksigen. Sel darah merah juga berperan dala
m penentuan \par\pard\qj \li2342\ri1553\sb0\sl-280\slmult0\fi360 \up0 \expndtw-2
\charscalex100 {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/Golon
gan_darah" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 golongan darah.}}}\ul0\nosup
ersub\cf1\f2\fs24 Orang yang kekurangan eritrosit menderita penyakit {\field{\*
\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/Anemia" }}{\fldrslt {\ul0\nosu
persub\cf1\f2\fs24 anemia.}}} \line \up0 \expndtw-3\charscalex100 \ul0\nosupersu
b\cf1\f2\fs24 \u-3913?\ul0\nosupersub\cf6\f7\fs24 \ul0\nosupersub\cf4\f5\fs24
{\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/Keping-keping_darah
" }}{\fldrslt {\ul0\nosupersub\cf4\f5\fs24 Keping-keping darah}}}\ul0\nosupersub
\cf4\f5\fs24 atau {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/T
rombosit" }}{\fldrslt {\ul0\nosupersub\cf4\f5\fs24 trombosit}}}\ul0\nosupersub\c
f1\f2\fs24 (0,6 - 1,0%) \par\pard\qj \li2342\ri2316\sb0\sl-300\slmult0\fi1067 \
up0 \expndtw-3\charscalex100 Trombosit bertanggung jawab dalam proses {\field{\*
\fldinst {HYPERLINK "http://id.wikipedia.org/w/index.php?title=Pembekuan_darah&a
ction=edit&redlink=1" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 pembekuan darah}}
} \line \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 \u-3913?\ul0\n
osupersub\cf6\f7\fs24 \ul0\nosupersub\cf4\f5\fs24 {\field{\*\fldinst {HYPERLI
NK "http://id.wikipedia.org/wiki/Sel_darah_putih" }}{\fldrslt {\ul0\nosupersub\c
f4\f5\fs24 Sel darah putih}}}\ul0\nosupersub\cf4\f5\fs24 atau {\field{\*\fldins
t {HYPERLINK "http://id.wikipedia.org/wiki/Leukosit" }}{\fldrslt {\ul0\nosupersu
b\cf4\f5\fs24 leukosit}}}\ul0\nosupersub\cf1\f2\fs24 (0,2%). \par\pard\qj \li27
02\ri1502\sb0\sl-276\slmult0\fi707 \up0 \expndtw-2\charscalex100 Leukosit bertan
ggung jawab terhadap {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki
/Sistem_imun" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 sistem imun}}}\ul0\nosupe
rsub\cf1\f2\fs24 tubuh dan bertugas \line \up0 \expndtw0\charscalex101 untuk me
musnahkan benda-benda yang dianggap asing dan berbahaya oleh \line \up0 \expndtw
-2\charscalex100 tubuh, misal virus atau bakteri. Leukosit bersifat amuboid atau
tidak memiliki \line \up0 \expndtw0\charscalex103 bentuk yang tetap. Orang
yang kelebihan leukosit menderita penyakit \line \up0 \expndtw0\charscalex10
4 {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/Leukimia" }}{\fldr
slt {\ul0\nosupersub\cf1\f2\fs24 leukimia,}}}\ul0\nosupersub\cf1\f2\fs24 sedang
kan orang yang kekurangan leukosit menderita penyakit \line \up0 \expndtw-3\char
scalex100 {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.org/w/index.php?tit
le=Leukopenia&action=edit&redlink=1" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 le
ukopenia.}}} \par\pard\qj \li2275\ri1498\sb0\sl-276\slmult0\fi708 \up0 \expndtw1\charscalex100 \ul0\nosupersub\cf4\f5\fs24 Susunan Darah\ul0\nosupersub\cf1\f2\
fs24 . serum darah atau plasma terdiri atas: {\field{\*\fldinst {HYPERLINK "http
://id.wikipedia.org/wiki/Air" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 Air:}}}\u
l0\nosupersub\cf1\f2\fs24 91,0%, {\field{\*\fldinst {HYPERLINK "http://id.wikip
edia.org/wiki/Protein" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 Protein:}}} \lin
e \up0 \expndtw0\charscalex103 \ul0\nosupersub\cf1\f2\fs24 8,0% (Albumin, globul

in, protrombin dan fibrinogen), {\field{\*\fldinst {HYPERLINK "http://id.wikiped
ia.org/wiki/Mineral" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 Mineral:}}}\ul0\no
supersub\cf1\f2\fs24 0.9% {\field{\*\fldinst {HYPERLINK "http://id.wikipedia.or
g/wiki/Natrium_klorida" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 (natrium}}} \li
ne \up0 \expndtw0\charscalex100 {\field{\*\fldinst {HYPERLINK "http://id.wikiped
ia.org/wiki/Natrium_klorida" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 klorida, n
atrium bikarbonat, garam}}}\ul0\nosupersub\cf1\f2\fs24 dari {\field{\*\fldinst
{HYPERLINK "http://id.wikipedia.org/wiki/Kalsium" }}{\fldrslt {\ul0\nosupersub\c
f1\f2\fs24 kalsium, fosfor,}}}\ul0\nosupersub\cf1\f2\fs24 magnesium dan zat bes
i, \line \up0 \expndtw0\charscalex102 dll). {\field{\*\fldinst {HYPERLINK "http:
//id.wikipedia.org/wiki/Plasma_darah" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 P
lasma darah}}}\ul0\nosupersub\cf1\f2\fs24 pada dasarnya adalah larutan {\field{
\*\fldinst {HYPERLINK "http://id.wikipedia.org/wiki/Air" }}{\fldrslt {\ul0\nosup
ersub\cf1\f2\fs24 air}}}\ul0\nosupersub\cf1\f2\fs24 yang mengandung albumin, \l
ine \up0 \expndtw0\charscalex100 bahan pembeku darah, {\field{\*\fldinst {HYPERL
INK "http://id.wikipedia.org/wiki/Antibodi" }}{\fldrslt {\ul0\nosupersub\cf1\f2\
fs24 immunoglobin}}}\ul0\nosupersub\cf1\f2\fs24 (antibodi), {\field{\*\fldinst
{HYPERLINK "http://id.wikipedia.org/wiki/Hormon" }}{\fldrslt {\ul0\nosupersub\cf
1\f2\fs24 hormon,}}}\ul0\nosupersub\cf1\f2\fs24 berbagai jenis protein, \line \
up0 \expndtw-3\charscalex100 berbagai jenis {\field{\*\fldinst {HYPERLINK "http:
//id.wikipedia.org/wiki/Garam" }}{\fldrslt {\ul0\nosupersub\cf1\f2\fs24 garam.}}
} \par\pard\ql \li2275\sb233\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\n
osupersub\cf2\f3\fs24 G.2.2 Antioksidan dalam darah \par\pard\qj \li2275\ri1501\
sb9\sl-270\slmult0\fi851 \up0 \expndtw0\charscalex100 \ul0\nosupersub\cf1\f2\fs2
4 Dalam pengertian klinis yang sering dianggap sebagai antioksidan total \line \
up0 \expndtw0\charscalex100 adalah antioksidan yang mempunai berat molekul besar
(primer) serta berfungsi \line \up0 \expndtw0\charscalex102 mencegah terbentuk
nya radikal bebas, dalam hal ini \up0 \expndtw0\charscalex104 : SOD \up0 \
expndtw-3\charscalex100 (\ul0\nosupersub\cf4\f5\fs24 Superoksida \par\pard\qj \l
i2275\ri1502\sb6\sl-275\slmult0 \up0 \expndtw0\charscalex104 dismutase\ul0\nosup
ersub\cf1\f2\fs24 ) dan \ul0\nosupersub\cf4\f5\fs24 Glutation peroksidase\ul0\no
supersub\cf1\f2\fs24 (GPx). Kadar mereka berkisar: 1,30 s/d \up0 \expndtw0\char
scalex100 1,77 mmol/L (SOD: 1,102 s/d 1,601 \u956?/g Hb, Gpx: 27,5 s/d 73,6 \u95
6?/g Hb). Secara \up0 \expndtw0\charscalex108 teoritis kalau kadar ini dalam dar
ah dapat ditentukan maka dapat diprediksi \up0 \expndtw0\charscalex100 adanya ba
haya radikal bebas, dengan sekaligus memberikannya antioksidan serta \up0 \expnd
tw-3\charscalex100 dapat mengikuti efektifitas berbagai pengobatan. \par\pard\se
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}
\pard\sb0\sl-240{\bkmkstart Pg5}{\bkmkend Pg5}\par\pard\ql \li10089\sb0\sl-276\s
lmult0 \par\pard\ql\li10089\sb155\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \
ul0\nosupersub\cf1\f2\fs24 5 \par\pard\ql \li2275\sb0\sl-276\slmult0 \par\pard\q
l\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\
li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb180\sl-276\slmult0 \up0 \expndtw
-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 G.3 Cahaya \par\pard\qj \li2275\ri1
503\sb4\sl-277\slmult0\fi720 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f
2\fs24 Gelombang cahaya merupakan gelombang elektromaknetik, yang biasanya \up0
\expndtw0\charscalex102 di karakteristik dengan panjang gelombang, cepat rambat
gelombang, frekuensi \up0 \expndtw0\charscalex100 gelombang dan sebagainya. Panj
ang gelombang didefinisikan sebagai jarak antar \up0 \expndtw0\charscalex103 dua
titik maksimum atau dua titik minimum yang berdekatan. Sedangkan \up0
\expndtw0\charscalex102 frekuensi di definisikan banyaknya gelombang yang melewa
ti suatu titik dalam \up0 \expndtw0\charscalex106 satu detik. Cepat ranbant gelo
mbang adalah jarak yang ditempuh gelombang \up0 \expndtw0\charscalex104 dalam sa
tu satuan waktu.Hubungan antara panjang gelombang, frekuensi, dan \up0 \expndtw3\charscalex100 cepat rambat gelombang adalah di nyatakan dengan: \par\pard\li29
83\sb0\sl-276\slmult0\par\pard\li2983\sb0\sl-276\slmult0\par\pard\li2983\sb40\sl
-276\slmult0\fi0\tx4436\tx5156 \up0 \expndtw-3\charscalex100 Dengan\tab \up0 \ex
pndtw-3\charscalex100 c\tab \up0 \expndtw-3\charscalex100 : cepat rambat gelomba
ng cahaya ( 3 x 10\ul0\super\cf8\f9\fs24 8 \ul0\nosupersub\cf1\f2\fs24 m/s)\par\
pard\li2983\sb0\sl-276\slmult0\fi1452\tx5156 \up0 \expndtw-3\charscalex100 f\tab
\up0 \expndtw-3\charscalex100 : frekuensi gelombang (Hz)\par\pard\li2983\sb0\sl
-276\slmult0\fi1452\tx5156 \up0 \expndtw-3\charscalex100 \u955?\tab \up0 \expndt
w-3\charscalex100 : panjang gelombang (m)\par\pard\qj \li2275\ri1500\sb0\sl-280\
slmult0\fi708 \up0 \expndtw0\charscalex100 Gelombang elektromagnetik mempuny
ai spektrum dengan rentang \up0 \expndtw0\charscalex105 panjang gelombang
yang sangat lebar yaitu mulai dari 10\ul0\super\cf8\f9\fs24 6 \ul0\nosupersub\c
f1\f2\fs24 sampai 10\ul0\super\cf8\f9\fs24 -12. \ul0\nosupersub\cf1\f2\fs24 Dari
\up0 \expndtw0\charscalex102 berbagai macam panjang gelombang yang ada hanya se
bagian kecil yang dapat \up0 \expndtw0\charscalex103 dilihat oleh mata yaitu pad
a rentang panjang gelombang tampak yang berkisar \up0 \expndtw-3\charscalex100 a

ntar 400 nm sampai 700 nm. \par\pard\ql \li2275\sb245\sl-276\slmult0 \up0 \expnd
tw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 G.4 Spektrofotometer \par\pard\qj
\li2275\ri1497\sb1\sl-280\slmult0\fi851 \up0 \expndtw0\charscalex102 \ul0\nosup
ersub\cf1\f2\fs24 Hukum beer menyatakan bahwa suatu larutan akan mengabso
rbsi \up0 \expndtw0\charscalex100 sejumlah energi tertentu apabila larutan terse
but dikenai radiasi elektromagnetik. \up0 \expndtw-3\charscalex100 Absorbsi yang
terjadi dapat dinyatakan dengan rumus : \par\pard\li2275\sb0\sl-276\slmult0\par
\pard\li2275\sb10\sl-276\slmult0\fi0\tx3715 \up0 \expndtw-3\charscalex100 Dengan
P\ul0\sub\cf8\f9\fs24 0\tab \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\
f2\fs24 : Intensitas radiasi yang mengenai larutan\par\pard\li2275\sb0\sl-276\sl
mult0\fi900\tx3715 \up0 \expndtw-3\charscalex100 P\tab \up0 \expndtw-3\charscale
x100 : intensitas radiasi yang diteruskan larutan\par\pard\li2275\sb0\sl-276\slm
ult0\fi900\tx3715 \up0 \expndtw-3\charscalex100 a\tab \up0 \expndtw-3\charscalex
100 : faktor absorbsi\par\pard\li2275\sb0\sl-276\slmult0\fi902\tx3715 \up0 \expn
dtw-3\charscalex100 L\tab \up0 \expndtw-3\charscalex100 : Panjang jalur yang dil
ewati cahaya\par\pard\li2275\sb1\sl-272\slmult0\fi900\tx3715 \up0 \expndtw-3\cha
rscalex100 C\tab \up0 \expndtw-3\charscalex100 : Konsentrasi larutan pengabsorbs
i\par\pard\qj \li2275\ri1503\sb0\sl-280\slmult0\fi851 \up0 \expndtw0\charscalex1
00 Besaran yang umum di pakai dalam spektrofotometri yang menyatakan \up0 \expnd
tw0\charscalex102 hubungan antara P dan P\ul0\sub\cf8\f9\fs24 0 \ul0\nosupersub\
cf1\f2\fs24 adalah \ul0\nosupersub\cf4\f5\fs24 transmittance \ul0\nosupersub\cf1
\f2\fs24 (T), biasanya dinyatakan dalam \up0 \expndtw-3\charscalex100 persen (%)
. Hubunga antara T, P, dan P\ul0\sub\cf8\f9\fs24 0 \ul0\nosupersub\cf1\f2\fs24
adalah sebagai berikut \par\pard\qj \li2275\sb0\sl-280\slmult0 \par\pard\qj\li22
75\ri1501\sb279\sl-280\slmult0\fi851 \up0 \expndtw0\charscalex104 Karena hubun
gan antara konsentrasi dan \ul0\nosupersub\cf4\f5\fs24 transmittance \ul
0\nosupersub\cf1\f2\fs24 adalah \up0 \expndtw0\charscalex104 eksponensial, mak
a akan lebih mudah menyatakan hubungan ini dengan suatu \up0 \expndtw-3\charscal
ex100 besaran yang di sebut \ul0\nosupersub\cf4\f5\fs24 absorbance \ul0\nosuper
sub\cf1\f2\fs24 (A), dengan persamaan: \par\pard\qj \li2275\sb0\sl-275\slmult0 \
par\pard\qj\li2275\sb0\sl-275\slmult0 \par\pard\qj\li2275\ri1498\sb35\sl-275\slm
ult0\fi851 \up0 \expndtw0\charscalex100 Besarnya absorbsi ditentukan oleh panjan
g gelombang yang digunakan. \up0 \expndtw0\charscalex102 Daerah panjang gelomb
ang dimana terjadi absorbsi maksimum merupakan \up0 \expndtw0\charscalex106
parameter penting untuk analisa kuantitatif dengan prinsip spektrofotometri \u
p0 \expndtw-2\charscalex100 karena pengukuran pada daerah ini akan memberikan hu
bungan yang linear antara \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf4\f5\f
s24 absorbance\ul0\nosupersub\cf1\f2\fs24 (A) dan konsentrasi (C). \par\pard\se
ct\sectd\fs24\paperw11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg6}{\bkmkend P
g6}\par\pard\ql \li10089\sb0\sl-276\slmult0 \par\pard\ql\li10089\sb155\sl-276\sl
mult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 6 \par\pard\qj \
li2275\sb0\sl-276\slmult0 \par\pard\qj\li2275\sb0\sl-276\slmult0 \par\pard\qj\li
2275\sb0\sl-276\slmult0 \par\pard\qj\li2275\sb0\sl-276\slmult0 \par\pard\qj\li22
75\ri1502\sb180\sl-276\slmult0\fi708 \up0 \expndtw0\charscalex104 Menurut \ul0\n
osupersub\cf4\f5\fs24 Cairns (2009),\ul0\nosupersub\cf1\f2\fs24 spektrofotomete
r adalah alat untuk mengukur \line \up0 \expndtw0\charscalex102 transmitan atau
absorban suatu sampel sebagai fungsi panjang gelombang. Tiap \line \up0 \expndtw
0\charscalex100 media akan menyerap cahaya pada panjang gelombang tertentu terga
ntung pada \line \up0 \expndtw0\charscalex102 senyawaan atau warna terbentuk.
Spektrofotometer dapat digunakan untuk \line \up0 \expndtw0\charscalex102 m
engetahui konsentrasi larutan dengan menggunakan prinsip spektrofotometri. \line
\up0 \expndtw0\charscalex105 Pengukuran kadar antioksidan dalam darah juga
dapat dilakukan dengan \line \up0 \expndtw0\charscalex104 menggunakan cara t
ersebut. Secara ringkas cara kerja dari alat tersebut adalah \line \up0 \expndtw
0\charscalex103 darah yang sudah dilarutkan dengan pelarut dengan perband
ingan tertentu \line \up0 \expndtw0\charscalex100 ditempatkan dalam sebuah kuve
t (Tempat sampel darah), kemudian berkas sinar \line \up0 \expndtw0\charscalex10
3 monokromatis dengan panjang gelombang 500 s/d 600 nm dilewatkan melalui \line
\up0 \expndtw0\charscalex103 kuvet tersebut. Setelah dilewatkan kuvet cahaya di
tangkap oleh sensor cahaya \line \up0 \expndtw-3\charscalex100 untuk diukur inte
nsitasnya \par\pard\ql \li3127\sb4\sl-276\slmult0 \up0 \expndtw-3\charscalex100
Secara garis besar spektrofotometer terdiri dari 4 penting yaitu : \par\pard\ql
\li2275\sb4\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\
fs24 G.4.1 Sumber Cahaya \par\pard\ql \li2983\sb1\sl-256\slmult0 \up0 \expndtw-3
\charscalex100 \ul0\nosupersub\cf1\f2\fs24 Sebagai sumber cahaya haruslah dalam
range cahaya dimana cahaya \par\pard\ql \li2275\ri1696\sb9\sl-275\slmult0 \up0 \
expndtw-2\charscalex100 terabsorbsi maksimum. Sumber cahaya diusahakan mempunyai
pancaran radiasi \line \up0 \expndtw-2\charscalex100 yang stabil dan intensitas
nya tinggi . Sumber cahaya yang digunakan harus \line \up0 \expndtw-2\charscalex
100 spesifik karena dalam metode spektrofotometri serat optik panjang gelombang
\line \up0 \expndtw-2\charscalex100 sumber cahaya yang digunakan harus sama ata
u setidaknya mendekati panjang \line \up0 \expndtw-3\charscalex100 gelombang se
rapan . \par\pard\ql \li3458\sb5\sl-276\slmult0 \up0 \expndtw-3\charscalex100 Ta
bel 1. panjang gelombang dari berbagai warna cahaya)\par\pard\sect\sectd\sbknon
e\cols3\colno1\colw4655\colsr160\colno2\colw2673\colsr160\colno3\colw4122\colsr1
60\ql \li2604\sb160\sl-276\slmult0\tx3458 \up0 \expndtw-3\charscalex100 No\tab \
up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 \u955? \ul0\nosupersub
\cf1\f2\fs24 (nm)\par\pard\ql \li2690\sb149\sl-276\slmult0\tx3302 \up0 \expndtw3\charscalex100 1\tab \up0 \expndtw0\charscalex101 400 - 435\par\pard\ql \li2690
\sb10\sl-276\slmult0\tx3302 \up0 \expndtw-3\charscalex100 2\tab \up0 \expndtw0\c
harscalex101 435 - 480\par\pard\ql \li2690\sb9\sl-276\slmult0\tx3302 \up0 \expnd
tw-3\charscalex100 3\tab \up0 \expndtw0\charscalex101 480 - 490\par\pard\ql \li2
690\sb10\sl-276\slmult0\tx3302 \up0 \expndtw-3\charscalex100 4\tab \up0 \expndtw
0\charscalex101 490 - 500\par\pard\ql \li2690\sb12\sl-276\slmult0\tx3302 \up0 \e
xpndtw-3\charscalex100 5\tab \up0 \expndtw0\charscalex101 500 - 560\par\pard\ql
\li2690\sb9\sl-276\slmult0\tx3302 \up0 \expndtw-3\charscalex100 6\tab \up0 \expn
dtw0\charscalex101 560 - 580\par\pard\ql \li2690\sb10\sl-276\slmult0\tx3302 \up0
\expndtw-3\charscalex100 7\tab \up0 \expndtw0\charscalex101 580 - 595\par\pard\
ql \li2690\sb9\sl-276\slmult0\tx3302 \up0 \expndtw-3\charscalex100 8\tab \up0 \e
xpndtw0\charscalex101 595 - 610\par\pard\qj \li2558\ri298\sb2\sl-286\slmult0\fi1
31\tx3302 \up0 \expndtw-3\charscalex100 9\tab \up0 \expndtw0\charscalex101 610 750 \line \up0 \expndtw-3\charscalex100 *) Warna larutan\par\pard\ql \li2275\sb
0\sl-276\slmult0 \par\pard\ql \li2275\sb4\sl-276\slmult0 \up0 \expndtw-3\charsca
lex100 \ul0\nosupersub\cf2\f3\fs24 G.4.2 Monokromator\par\pard\column \ql \li20\
sb160\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 W
arna yang terabsobsi\par\pard\qj \li819\ri1175\sb142\sl-285\slmult0\tx915 \up0 \
expndtw-3\charscalex100 Violet \line\tab \up0 \expndtw-3\charscalex100 Biru\par\
pard\qj \li519\ri877\sb0\sl-285\slmult0 \up0 \expndtw0\charscalex100 Hijau - Bir
u \line \up0 \expndtw-3\charscalex100 Biru - Hijau\par\pard\ql \li853\sb11\sl-27
6\slmult0 \up0 \expndtw-3\charscalex100 Hijau\par\pard\qj \li392\ri749\sb2\sl-28
5\slmult0\tx769 \up0 \expndtw-3\charscalex100 Kuning - Hijau \line\tab \up0 \exp
ndtw-3\charscalex100 Kuning\par\pard\qj \li805\ri1164\sb0\sl-285\slmult0 \up0 \e
xpndtw-3\charscalex100 Jingga \line \up0 \expndtw-3\charscalex100 Merah\par\pard
\column \qj \li20\ri1992\sb24\sl-275\slmult0\tx342 \up0 \expndtw-3\charscalex100
Warna tertransmisi*) \line\tab \up0 \expndtw-3\charscalex100 (Komplemen)\par\pa
rd\qj \li257\ri2295\sb3\sl-285\slmult0\tx654 \up0 \expndtw0\charscalex100 Kuning
- Hijau \line\tab \up0 \expndtw-3\charscalex100 Kuning\par\pard\qj \li692\ri272
6\sb0\sl-286\slmult0 \up0 \expndtw-3\charscalex100 Jingga \line \up0 \expndtw-3\
charscalex100 Merah \line \up0 \expndtw-4\charscalex100 Ungu \line \up0 \expndtw
-3\charscalex100 Violet\par\pard\ql \li790\sb9\sl-276\slmult0 \up0 \expndtw-3\ch
arscalex100 Biru\par\pard\qj \li404\ri2440\sb2\sl-285\slmult0 \up0 \expndtw0\cha
rscalex100 Hijau - Biru \line \up0 \expndtw0\charscalex100 Biru - Hijau \par\par
d\sect\sectd\sbknone \qj \li2275\ri1503\sb0\sl-270\slmult0\fi708 \up0 \expndtw0\
charscalex105 Monokromator adalah alat yang berfungsi untuk menguraikan cahaya \
up0 \expndtw0\charscalex104 polikromatis menjadi beberapa komponen panja
ng gelombang tertentu \up0 \expndtw-3\charscalex100 (monokromatis) yang bebe
da (terdispersi). \par\pard\ql \li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb
7\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 G.4.3
Cuvet \par\pard\qj \li2275\ri1502\sb5\sl-275\slmult0\fi708 \up0 \expndtw0\chars
calex100 \ul0\nosupersub\cf1\f2\fs24 Cuvet spektrofotometer adalah suatu alat ya

ng digunakan sebagai tempat \line \up0 \expndtw0\charscalex104 contoh atau cupli
kan yang akan dianalisis. Cuvet biasanya terbuat dari kwars, \line \up0 \expndt
w0\charscalex100 plexigalass, kaca, plastic dengan bentuk tabung empat persegi p
anjang 1 x 1 cm \line \up0 \expndtw0\charscalex108 dan tinggi 5 cm. Pada penguku
ran di daerah UV dipakai cuvet kwarsa atau \line \up0 \expndtw0\charscalex100 pl
exiglass, sedangkan cuvet dari kaca tidak dapat dipakai sebab
kaca
{\shp {\*\shpinst\shpleft2494\shptop8053\shpright2504\shpbottom8063\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz510\shplid0
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{\shp {\*\shpinst\shpleft9983\shptop8063\shpright10003\shpbottom8616\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz540\shplid15
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{\shp {\*\shpinst\shpleft9983\shptop10055\shpright10003\shpbottom10332\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz1676\shplid99

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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16820\pard\sb0\sl-240{\bkmkstart P
g7}{\bkmkend Pg7}\par\pard\ql \li10089\sb0\sl-276\slmult0 \par\pard\ql\li10089\s
b155\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 7
\par\pard\qj \li2275\sb0\sl-280\slmult0 \par\pard\qj\li2275\sb0\sl-280\slmult0 \
par\pard\qj\li2275\sb0\sl-280\slmult0 \par\pard\qj\li2275\sb0\sl-280\slmult0 \pa
r\pard\qj\li2275\ri1505\sb161\sl-280\slmult0 \up0 \expndtw0\charscalex100 mengab
sorbsi sinar UV. Semua macam cuvet dapat dipakai untuk pengukuran di \up0 \expnd
tw-3\charscalex100 daerah sinar tampak (visible). \par\pard\ql \li2275\sb0\sl-27
6\slmult0 \par\pard\ql\li2275\sb8\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \
ul0\nosupersub\cf2\f3\fs24 G.4.4 Sensor cahaya \par\pard\qj \li2275\ri1502\sb0\s
l-280\slmult0\fi708 \up0 \expndtw0\charscalex106 \ul0\nosupersub\cf1\f2\fs24 Per
anan sensor cahaya penerima adalah memberikan respon terhadap \up0 \expndtw-1\ch
arscalex100 cahaya pada berbagai panjang gelombang. sensor akan mengubah cahaya
menjadi \up0 \expndtw-2\charscalex100 sinyal listrik yang selanjutnya di proses
dikomputer. \par\pard\ql \li2275\sb265\sl-276\slmult0 \up0 \expndtw-3\charscalex
100 \ul0\nosupersub\cf2\f3\fs24 G.5 Operasional Amplifier (Op- Amp) \par\pard\q
j \li2275\ri1501\sb0\sl-280\slmult0\fi851 \up0 \expndtw0\charscalex100 \ul0\nosu
persub\cf1\f2\fs24 Operasional ampifier (Op-Amp) merupakan rangkaian elektronika

yang \line \up0 \expndtw0\charscalex108 didesain agar dapat digunakan dalam ber
bagai tujuan hanya dengan sedikit \line \up0 \expndtw0\charscalex100 menambahkan
komponen luar. Op Amp mempunyai 5 terminal dasar , yaitu: dua \line \up0 \expnd
tw-2\charscalex100 untuk suplay daya,dua untuk masukan(input), satu untuk keluar
an (output). \par\pard\qj \li2342\ri3073\sb0\sl-280\slmult0\fi784 \up0 \expndtw3\charscalex100 Op-Amp yang idea adalah mempunyai sifat-sifat berikut: \up0 \exp
ndtw-3\charscalex100 \u-3913?\ul0\nosupersub\cf6\f7\fs24 \ul0\nosupersub\cf1\f2
\fs24 Impedansi input yang tinggi. \par\pard\qj \li2342\ri2009\sb0\sl-300\slmu
lt0 \up0 \expndtw-2\charscalex100 \u-3913?\ul0\nosupersub\cf6\f7\fs24 \ul0\nosu
persub\cf1\f2\fs24 Impedansi output yang rendah, sehinga tidak ada pengaruh pe
mbebanan. \up0 \expndtw-3\charscalex100 \u-3913?\ul0\nosupersub\cf6\f7\fs24 \ul
0\nosupersub\cf1\f2\fs24 Mempunyai penguat yang sangat tinggi. \par\pard\ql \l
i2342\sb1\sl-257\slmult0 \up0 \expndtw-3\charscalex100 \u-3913?\ul0\nosupersub\c
f6\f7\fs24 \ul0\nosupersub\cf1\f2\fs24 Mempunyai bandwidth yang lebar. \par\p
ard\ql \li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb12\sl-276\slmult0 \up0 \
expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 G.6 ADC (Analog To Digital C
onverter) \par\pard\qj \li2275\ri1498\sb7\sl-273\slmult0\fi708 \up0 \expndtw0\ch
arscalex104 \ul0\nosupersub\cf1\f2\fs24 ADC digunakan untuk mengubah sinyal-siny
al analog menjadi sinyal-\line \up0 \expndtw0\charscalex104 sinyal digital yang
berupa kode-kode biner, sehingga dapat digunakan sebagai \line \up0 \expndtw0\ch
arscalex105 data yang dapat diolah komputer dengan bantuan perangkagkat lunak. F
aktor \line \up0 \expndtw0\charscalex104 penting yang menentukan spesifikasi A
DC adalah resolusi. Resolusi adalah \line \up0 \expndtw0\charscalex100 jumlah
level teganagan output yang dapat dihasilkan oleh ADC. Sebagai contoh \line \up0
\expndtw0\charscalex102 ADC dengan resolusi 8 bit dapat menghasilkan 256 level
tegangan output yang \line \up0 \expndtw-3\charscalex100 berbeda. \par\pard\qj \
li2275\ri1500\sb5\sl-276\slmult0\fi708 \up0 \expndtw0\charscalex106 Untuk tujuan
mengkonversi sinyal-sinyal tegangan yang ada menjadi \line \up0 \expndtw0\chars
calex100 sinyal-sinyal teganagan yang kita inginkan dapat digunakan deferensial
applifier. \line \up0 \expndtw-1\charscalex100 Deferensial amplifier merupakan m
erupakan suatu penguat deferensial yang bisa \line \up0 \expndtw-1\charscalex10
0 mengukur maupun memperkuat sinyal-sinyal yang ada hingga menjadi jauh lebih \l
ine \up0 \expndtw0\charscalex104 besar dan sesuai dengan yang diinginkan. Peruba
han level tegangan output ini \line \up0 \expndtw0\charscalex107 menunjukkan p
erubahan tiap bit dari \up0 \expndtw0\charscalex103 00000000 sampai \up0
\expndtw-3\charscalex100 11111111. \par\pard\qj \li2275\ri1503\sb1\sl-280\slmul
t0 \up0 \expndtw0\charscalex103 Sedangkanperbedaan tegangan tiap bit-nya ter
gantung dari besar tegangan \up0 \expndtw-3\charscalex100 inputnya. \par\pard
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-2\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.\ul0\nosupersub\cf3\f4\fs24 \ul0
\nosupersub\cf2\f3\fs24 METODE PELAKSANAAN \par\pard\ql \li2275\sb4\sl-276\slmul
t0 \up0 \expndtw-3\charscalex100 H.1 Waktu Dan Tempat Penelitian \par\pard\qj \l
i2275\ri1497\sb5\sl-275\slmult0\fi851 \up0 \expndtw-1\charscalex100 \ul0\nosuper
sub\cf1\f2\fs24 Penelitian ini akan dilaksanakan mulai bulan Februari sampai Ju
ni 2011. \up0 \expndtw0\charscalex100 Rencana perancangan spektrofotometer secar

a keseluruhan akan dilaksanakan di \up0 \expndtw0\charscalex103 laboratorium fi
sika Instrumentasi Unair. Pengambilan sampel darah untuk \up0 \expndtw0\ch
arscalex103 pengujian akan di rencanakan di laboratorium Larissa, yaitu laborato
rium yang \up0 \expndtw-3\charscalex100 sudah diakui di daerah surabaya. \par\pa
rd\ql \li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb9\sl-276\slmult0 \up0 \ex
pndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.2 Bahan Dan Alat Penelitian
\par\pard\ql \li3043\sb1\sl-256\slmult0\tx8319 \up0 \expndtw-1\charscalex100 \ul
0\nosupersub\cf1\f2\fs24 Bahan yang digunakan dalam penelitian ini adalah \ta
b \up0 \expndtw-1\charscalex100 : Darah dan pelarut \par\pard\ql \li2275\sb8\sl276\slmult0 \up0 \expndtw0\charscalex103 darah. Adapun komponen komponen dasar a
lat yang digunakan adalah sumber\par\pard\sect\sectd\sbknone\cols2\colno1\colw80
72\colsr110\colno2\colw3578\colsr160\qj \li2275\ri0\sb14\sl-275\slmult0 \up0 \ex
pndtw0\charscalex100 cahaya, monokromator, sensor cahaya, Op-Amp, ADC \line
\up0 \expndtw-3\charscalex100 Converter), dan komponen elektronik pendukung lai
nnya.\par\pard\ql \li2275\sb0\sl-276\slmult0 \par\pard\ql \li2275\sb6\sl-276\slm
ult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.3 Tahap Penelit
ian\par\pard\ql \li4592\sb0\sl-276\slmult0 \par\pard\ql \li4592\sb0\sl-276\slmul
t0 \par\pard\ql \li4592\sb0\sl-276\slmult0 \par\pard\ql \li4592\sb0\sl-276\slmul
t0 \par\pard\ql \li4592\sb0\sl-276\slmult0 \par\pard\ql \li4592\sb78\sl-276\slmu
lt0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 Gambar 1. Diagram
alir penelitian\par\pard\ql \li2275\sb0\sl-276\slmult0 \par\pard\ql \li2275\sb0\
sl-276\slmult0 \par\pard\ql \li2275\sb5\sl-276\slmult0 \up0 \expndtw-3\charscale
x100 \ul0\nosupersub\cf2\f3\fs24 H.4.1 Tahap Persiapan\par\pard\column \ql \li20
\sb13\sl-276\slmult0 \up0 \expndtw0\charscalex100 \ul0\nosupersub\cf1\f2\fs24 (A
nalog To Digital \par\pard\sect\sectd\sbknone \qj \li2275\ri1498\sb0\sl-275\sl
mult0\fi851 \up0 \expndtw0\charscalex102 Pada tahap ini yang perlu dilakukan
adalah menyiapkan komponen\up0 \expndtw0\charscalex103 komponen pendukung pembu
atan alat sumber cahaya, monokromator, Sensor \up0 \expndtw-1\charscalex100 caha
ya, Op-Amp, ADC (Analog To Digital Converter) dan komponen elektronik \up0 \exp
ndtw0\charscalex105 pendukung lainnya. Komponen-komponen tersebut dapat diperole
h dari toko \up0 \expndtw-3\charscalex100 optik dan elektronika di Surabaya. \pa
r\pard\sect\sectd\fs24\paperw11900\paperh16820
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-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.4.2 Perakitan alat \par\pard\qj \
li2275\ri1500\sb4\sl-276\slmult0\fi708 \up0 \expndtw0\charscalex101 \ul0\nosuper
sub\cf1\f2\fs24 Sumber cahaya yang digunakan harusnya cahaya monokromatis dengan
\line \up0 \expndtw-1\charscalex100 panjang gelombang 500 s/d 600 nm. Panjang g
elombang tersebut termasuk dalam \line \up0 \expndtw0\charscalex103 cahaya tampa
k, warna cahaya yang masuk dalam range tersebut adalah cahaya \line \up0 \expndt
w0\charscalex105 warna hijau dengan panjang gelombang 500 s/d 590 nm. Sehingga d
igunakan \line \up0 \expndtw0\charscalex105 filter warna hijau untuk memperoleh
cahaya monokromatis yang meloloskan \line \up0 \expndtw0\charscalex103 cahaya d
engan panjang gelombang \up0 \expndtw0\charscalex103 500-560 nm. Perancangan
komponen \par\pard\qj \li2275\ri1500\sb9\sl-270\slmult0 \up0 \expndtw0\charsca
lex100 elektronik secara keseluruhan adalah menggabungkan antara sensor c
ahaya, \up0 \expndtw0\charscalex100 Rangkaian Op-amp, ADC(Analog to Digital Conv
erter), dan Komputer. Adapun \up0 \expndtw-3\charscalex100 blok diagram alat sec
ara keseluruhan sebagai berikut : \par\pard\ql \li5199\sb0\sl-276\slmult0 \par\p
ard\ql\li5199\sb0\sl-276\slmult0 \par\pard\ql\li5199\sb0\sl-276\slmult0 \par\par
d\ql\li5199\sb0\sl-276\slmult0 \par\pard\ql\li5199\sb0\sl-276\slmult0 \par\pard\
ql\li5199\sb0\sl-276\slmult0 \par\pard\ql\li5199\sb0\sl-276\slmult0 \par\pard\ql
\li5199\sb0\sl-276\slmult0 \par\pard\ql\li5199\sb0\sl-276\slmult0 \par\pard\ql\l
i5199\sb0\sl-276\slmult0 \par\pard\ql\li5199\sb0\sl-276\slmult0 \par\pard\ql\li5
199\sb190\sl-276\slmult0 \up0 \expndtw-3\charscalex100 Gambar 2. Blok diagram al
at \par\pard\qj \li2275\ri1502\sb5\sl-275\slmult0\fi708 \up0 \expndtw0\charscale
x108 Supaya kode biner dari ADC dapat dibaca oleh komputer kemudian \line \up0 \
expndtw0\charscalex102 diproses dan di tampilkan oleh komputer sesuai dengan yan
g diharapkan, maka \line \up0 \expndtw0\charscalex102 harus ada software yang da
pat melakukan itu semua di dalam komputer. Untuk \line \up0 \expndtw0\charscalex
106 melakuakan itu semua dalam penelitian ini digunakan bahasa pemrograman \line
\up0 \expndtw-3\charscalex100 delphi 7. \par\pard\ql \li2275\sb0\sl-276\slmult0
\par\pard\ql\li2275\sb9\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosup
ersub\cf2\f3\fs24 H.4.3 Uji Coba Kinerja Alat \par\pard\qj \li2275\ri1497\sb18\s
l-260\slmult0\fi708 \up0 \expndtw0\charscalex101 \ul0\nosupersub\cf1\f2\fs24 Uji
kinerja alat dilakukan dengan menguji pe blok kemudian dilanjutkan \up0 \expndt
w-3\charscalex100 dengan menguji seluruh peralatan. Urutan pengujianya sebagai b

erikut: \par\pard\qj \li2342\ri1508\sb4\sl-280\slmult0\tx2702 \up0 \expndtw0\cha
rscalex104 1.\ul0\nosupersub\cf6\f7\fs24 \ul0\nosupersub\cf1\f2\fs24 pengujian
blok rangkaian penguat Op-Amp \ul0\nosupersub\cf4\f5\fs24 non Inverting\ul0\nos
upersub\cf1\f2\fs24 dilakukan untuk \line\tab \up0 \expndtw0\charscalex106 mend
apatkan penguatan antara tegangan masukan dari sensor \up0 \expndtw0\charscalex1
00 (\ul0\nosupersub\cf4\f5\fs24 V\ul0\sub\cf11\f12\fs24 in\ul0\nosupersub\cf1\f2
\fs24 ) dan \par\pard\qj \li2702\ri1498\sb0\sl-280\slmult0 \up0 \expndtw0\chars
calex106 tegangan keluaran dari op- amp (\ul0\nosupersub\cf4\f5\fs24 V\ul0\sub\c
f11\f12\fs24 out\ul0\nosupersub\cf1\f2\fs24 ) dengan hubungan \ul0\nosupersub\c
f4\f5\fs24 V\ul0\sub\cf11\f12\fs24 out\ul0\nosupersub\cf4\f5\fs24 = a Vin \ul0\
nosupersub\cf1\f2\fs24 , a \up0 \expndtw-3\charscalex100 adalah gain atau pengua
t dai op-amp. \par\pard\ql \li2342\sb1\sl-255\slmult0 \up0 \expndtw0\charscalex1
02 2.\ul0\nosupersub\cf6\f7\fs24 \ul0\nosupersub\cf1\f2\fs24 Pengujian blok ra
ngkaian penguat logaritmik dilakukan untuk mendapatkan\par\pard\sect\sectd\sbkno
ne\cols2\colno1\colw6673\colsr110\colno2\colw4977\colsr160\qj \li2702\ri0\sb0\sl
-359\slmult0\tx4831 \up0 \expndtw0\charscalex101 hubungan logaritmik antara m
asukan \line\tab \up0 \expndtw-9\charscalex75 \ul0\nosupersub\cf12\f13\fs24 \u-3
863?\ul0\nosupersub\cf14\f15\fs24 V \up0 \expndtw-2\charscalex100 \ul0\nosupersu
b\cf13\f14\fs14 in \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf14\f15\fs24 R
\up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf15\f16\fs14 3 \up0 \expndtw0\ch
arscalex80 \ul0\nosupersub\cf12\f13\fs24 \u-3847?\par\pard\column \ql \li20\sb21
\sl-276\slmult0 \up0 \expndtw0\charscalex103 \ul0\nosupersub\cf1\f2\fs24 (\ul0\n
osupersub\cf4\f5\fs24 V\ul0\sub\cf11\f12\fs24 i\ul0\nosupersub\cf1\f2\fs24 ) da
n keluaran \up0 \expndtw0\charscalex101 (\ul0\nosupersub\cf4\f5\fs24 V\ul0\sub
\cf11\f12\fs24 out\ul0\nosupersub\cf1\f2\fs24 ) dengan\par\pard\sect\sectd\sbkn
one\cols4\colno1\colw4588\colsr40\colno2\colw609\colsr40\colno3\colw303\colsr40\
colno4\colw6160\colsr160\ql \li2702\sb0\sl-216\slmult0 \up0 \expndtw-3\charscale
x100 hubungan \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf14\f15\fs24 V\up0
\expndtw0\charscalex107 \ul0\nosupersub\cf13\f14\fs14 out \up0 \expndtw-10\chars
calex80 \ul0\nosupersub\cf12\f13\fs24 \u-4035? \up0 \expndtw-3\charscalex100 \ul
0\nosupersub\cf14\f15\fs24 a\ul0\nosupersub\cf13\f14\fs14 T\par\pard\column \ql
\li20\ri0\sb0\sl-216\slmult0\tx233 \up0 \expndtw-9\charscalex82 \ul0\nosupersub\
cf12\f13\fs24 ln\u-3862? \line\tab \up0 \expndtw-9\charscalex86 \u-3861?\ul0\nos
upersub\cf14\f15\fs24 R \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf15\f16\f
s14 4\par\pard\column \ql \li20\sb76\sl-276\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf14\f15\fs24 V\up0 \expndtw-6\charscalex100 \ul0\nosupersub\c
f13\f14\fs14 cc\par\pard\column \qj \li20\ri5967\sb19\sl-192\slmult0 \up0 \expnd
tw0\charscalex80 \ul0\nosupersub\cf12\f13\fs24 \u-3846? \line \up0 \expndtw0\cha
rscalex80 \u-3845? \par\pard\sect\sectd\sbknone \qj \li2342\ri1500\sb0\sl-280\sl
mult0\tx2702 \up0 \expndtw0\charscalex106 \ul0\nosupersub\cf1\f2\fs24 3.\ul0\nos
upersub\cf6\f7\fs24 \ul0\nosupersub\cf1\f2\fs24 Pengujian blok rangkaian
penguat instrumentasi dilakukan untuk \line\tab \up0 \expndtw0\charscalex
103 mendapatkan hubungan logaritmik antara masukan (\ul0\nosupersub\cf4\f5\fs24
V\ul0\sub\cf11\f12\fs24 i\ul0\nosupersub\cf1\f2\fs24 ) dan keluaran (\ul0\nosupe
rsub\cf4\f5\fs24 V\ul0\sub\cf11\f12\fs24 o\ul0\nosupersub\cf1\f2\fs24 ) \par\par
d\li2702\sb13\sl-276\slmult0\fi0\tx4537\tx4850\tx5270\tx5528\tx6051 \up0 \expndt
w-3\charscalex100 dengan hubungan\tab \up0 \expndtw-3\charscalex100 \ul0\nosuper
sub\cf17\f18\fs24 V\t \dn2 \expndtw-2\charscalex100 \ul0\nosupersub\cf16\f17\fs1
4 o\tab \up0 \expndtw-8\charscalex100 \ul0\nosupersub\cf18\f19\fs24 \u-4035?\ul0
\nosupersub\cf17\f18\fs24 V\tab \dn2 \expndtw0\charscalex101 \ul0\nosupersub\cf1
9\f20\fs14 Re\t \dn2 \expndtw-2\charscalex100 \ul0\nosupersub\cf16\f17\fs14 f\ta
b \up0 \expndtw-11\charscalex88 \ul0\nosupersub\cf18\f19\fs24 \u-4051?\ul0\nosup
ersub\cf17\f18\fs24 cV\tab \dn2 \expndtw-1\charscalex100 \ul0\nosupersub\cf16\f
17\fs14 in\par\pard\qj \li2342\ri1505\sb95\sl-260\slmult0\tx2702 \up0 \expndtw0\
charscalex103 \ul0\nosupersub\cf1\f2\fs24 4.\ul0\nosupersub\cf6\f7\fs24 \ul0\no
supersub\cf1\f2\fs24 Pengujian blok rangkaian pengali dilakukan untuk mendapatk
an hubungan \line\tab \up0 \expndtw0\charscalex103 linier antara tegangan kel
uaran dari penguat logaritmik dengan besar \par\pard\sect\sectd\fs24\paperw
11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg10}{\bkmkend Pg10}\par\pard\ql \l
i9969\sb0\sl-276\slmult0 \par\pard\ql\li9969\sb155\sl-276\slmult0 \up0 \expndtw3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 10\par\pard\sect\sectd\sbknone\cols2

\colno1\colw6203\colsr40\colno2\colw5517\colsr160\ql \li2702\sb0\sl-276\slmult0
\par\pard\ql \li2702\sb0\sl-276\slmult0 \par\pard\ql \li2702\sb0\sl-276\slmult0
\par\pard\ql \li2702\sb0\sl-276\slmult0 \par\pard\ql \li2702\sb0\sl-276\slmult0
\par\pard\ql \li2702\sb89\sl-276\slmult0 \up0 \expndtw-3\charscalex100 konsentra
si dengan hubungan \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf20\f21\fs24 V
\up0 \expndtw0\charscalex108 \ul0\nosupersub\cf21\f22\fs14 out \up0 \expndtw-10\
charscalex80 \ul0\nosupersub\cf22\f23\fs24 \u-4035?\par\pard\column \qj \li6243\
sb0\sl-339\slmult0 \par\pard\qj \li6243\sb0\sl-339\slmult0 \par\pard\qj \li6243\
sb0\sl-339\slmult0 \par\pard\qj \li20\ri4795\sb250\sl-339\slmult0\tx291 \up0 \ex
pndtw0\charscalex108 \ul0\nosupersub\cf20\f21\fs24 V\ul0\nosupersub\cf21\f22\fs1
4 in\ul0\nosupersub\cf22\f23\fs24 \u-4051?\ul0\nosupersub\cf20\f21\fs24 b \line\
tab \up0 \expndtw-3\charscalex100 a \par\pard\sect\sectd\sbknone \ql \li2995\sb1
\sl-249\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 Langka
h langkah dalam uji coba alat adalah sebagai berikut: \par\pard\qj \li2342\ri150
3\sb0\sl-280\slmult0\tx2702 \up0 \expndtw0\charscalex102 1.\ul0\nosupersub\cf6\f
7\fs24 \ul0\nosupersub\cf1\f2\fs24 Mengukur tegangan keluaran yang dihasilkan
oleh rangkaian sensor cahaya \line\tab \up0 \expndtw-3\charscalex100 terhadap ka
dar antioksidan. \par\pard\qj \li2342\ri1500\sb0\sl-280\slmult0\tx2702 \up0 \exp
ndtw0\charscalex102 2.\ul0\nosupersub\cf6\f7\fs24 \ul0\nosupersub\cf1\f2\fs24
Mengukur tegangan keluaran yang dihasilkan oleh rangkaian penguat kadar \line\ta
b \up0 \expndtw-3\charscalex100 antioksidan. \par\pard\ql \li2275\sb270\sl-276\s
lmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.4.4 Pengambil
an Data \par\pard\qj \li2275\ri1497\sb0\sl-280\slmult0\fi720 \up0 \expndtw0\char
scalex105 \ul0\nosupersub\cf1\f2\fs24 Data yang diperoleh dari percobaan ini ber
upa nilai kadar antioksidan \up0 \expndtw0\charscalex104 dalam sampel darah dala
m satuan ppm dan intensitas radiasi transmitan yang \up0 \expndtw-3\charscalex1
00 diterima sensor cahaya keluarannya berupa tegangan dalam satuan volt. \par\pa
rd\ql \li3389\sb245\sl-276\slmult0 \up0 \expndtw-3\charscalex100 Tabel 3. Tabel
Ompong data pengukuran kadar antioksidan \par\pard\li2458\sb28\sl-276\slmult0\fi
0\tx3199\tx6157 \up0 \expndtw-3\charscalex100 No.\tab \up0 \expndtw-3\charscalex
100 Kadar antioksidan\tab \up0 \expndtw-3\charscalex100 Pembacaan Tegangan Kelua
ran\par\pard\li2458\sb0\sl-276\slmult0\fi1324\tx6049\tx7818\tx9383 \up0 \expndtw
-3\charscalex100 (ppm)\tab \up0 \expndtw-3\charscalex100 1\tab \up0 \expndtw-3\c
harscalex100 2\tab \up0 \expndtw-3\charscalex100 3\par\pard\ql \li2275\sb0\sl-27
6\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\
slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\sl
mult0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmu
lt0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb0\sl-276\slmult
0 \par\pard\ql\li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\sb104\sl-276\slmult
0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.4.5 Variabel Pene
litian \par\pard\qj \li2275\ri1499\sb9\sl-270\slmult0\fi720 \up0 \expndtw0\chars
calex104 \ul0\nosupersub\cf1\f2\fs24 Pada penelitian ini kadar antioksidan dalam
darah merupakan variabel \up0 \expndtw0\charscalex100 bebas, sedangkan beda int
ensitas antara I\ul0\sub\cf8\f9\fs24 0\ul0\nosupersub\cf1\f2\fs24 dan I merupak
an variabel terikat dan \up0 \expndtw-3\charscalex100 tegangan keluaran sebagai
variabel terukur. \par\pard\ql \li2275\sb0\sl-276\slmult0 \par\pard\ql\li2275\s
b10\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf2\f3\fs24 H.4
.6 Analisis data \par\pard\qj \li2275\ri1499\sb5\sl-275\slmult0\fi720 \up0 \expn
dtw-1\charscalex100 \ul0\nosupersub\cf1\f2\fs24 Data\ul0\nosupersub\cf2\f3\fs24
- \ul0\nosupersub\cf1\f2\fs24 data yang diperoleh dari pengambilan data dinyata
kan\ul0\nosupersub\cf2\f3\fs24 \ul0\nosupersub\cf1\f2\fs24 dalam grafik \up0 \e
xpndtw0\charscalex103 tegangan keluaran terhadap kadar antioksidan. Tegangan kel
uaran menyatakan \up0 \expndtw0\charscalex100 besarnya Kadar antioksidan dala
m darah. Semakin besar kadar antioksidan \up0 \expndtw0\charscalex102 semak
in besar pula tegangan keluaran. Grafik tersebut dianalisis dengan regresi \up0
\expndtw-3\charscalex100 linear y = ax +b kemudian dihitung koefisien korelasi g
rafik tersebut. \par\pard\qj \li2275\ri1503\sb10\sl-270\slmult0\fi708 \up0 \expn
dtw0\charscalex100 Setelah mengetahui hubungan kadar antioksidan dengan tegangan
keluar \up0 \expndtw0\charscalex104 alat. maka dari hubungan tersebut dibuatkan

program dengan bahasa program \up0 \expndtw-3\charscalex100 delphi 7 untuk mend
isplaikan hasil pengukuran di layar komputer.
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz35\shplid0{\sp{\sn shapeType}{\s
v 20}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}{\sp{\sn lineW
idth}{\sv 5616}}
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rsub\cf2\f3\fs24 I.\ul0\nosupersub\cf3\f4\fs24 \ul0\nosupersub\cf2\f3\fs24 JA
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wal pelaksanaan penelitian \par\pard\li3281\sb14\sl-276\slmult0\fi0\tx6982 \up0
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0 IV\tab \up0 \expndtw-4\charscalex100 V\par\pard\qj \li3046\ri7322\sb53\sl-720\
slmult0 \up0 \expndtw-3\charscalex100 Persiapan alat \line \up0 \expndtw-3\chars
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065\sb91\sl-276\slmult0 \up0 \expndtw-3\charscalex100 Studi pustaka \par\pard\ql
\li2858\sb0\sl-276\slmult0 \par\pard\ql\li2858\sb188\sl-276\slmult0 \up0 \expnd
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\pard\li2858\sb9\sl-276\slmult0\fi120\tx3660\tx8235 \up0 \expndtw-3\charscalex10
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rd\li2858\sb9\sl-276\slmult0\fi120\tx3660\tx8235 \up0 \expndtw-3\charscalex100 5
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charscalex100 250.000\par\pard\li2858\sb10\sl-276\slmult0\fi1524\tx8144 \up0 \ex
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i Arif \line\ul0\tab \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 N

IM 080710243\par\pard\qj \li5876\sb0\sl-275\slmult0 \par\pard\qj \li5876\sb0\sl275\slmult0 \par\pard\qj \li20\ri4398\sb3\sl-275\slmult0 \up0 \expndtw-3\charsca
lex100 : Abdul Halim \line \up0 \expndtw-3\charscalex100 : 080710242\par\pard\qj
\li20\ri3547\sb1\sl-276\slmult0 \up0 \expndtw-3\charscalex100 : FSAINSTEK/Fisik
a \up0 \expndtw-3\charscalex100 : Universitas Airlangga \up0 \expndtw-3\charscal
ex100 : \up0 \expndtw-3\charscalex100 9 Jam/Minggu\par\pard\qj \li8204\sb0\sl-27
5\slmult0 \par\pard\qj \li8204\sb0\sl-275\slmult0 \par\pard\qj \li8204\sb0\sl-27
5\slmult0 \par\pard\qj \li8204\sb0\sl-275\slmult0 \par\pard\qj \li2348\ri1853\sb
6\sl-275\slmult0\fi172 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf24\f25\fs
24\ul Abdul Halim \line \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs2
4 NIM 080710242\par\pard\qj \li5876\sb0\sl-275\slmult0 \par\pard\qj \li5876\sb0\
sl-275\slmult0 \par\pard\qj \li5876\sb0\sl-275\slmult0 \par\pard\qj \li5876\sb0\
sl-275\slmult0 \par\pard\qj \li5876\sb0\sl-275\slmult0 \par\pard\qj \li20\ri4374
\sb6\sl-275\slmult0 \up0 \expndtw-3\charscalex100 : Yopi Cahyo \up0 \expndtw-3
\charscalex100 : \up0 \expndtw-3\charscalex100 080710276\par\pard\qj \li20\ri354
7\sb1\sl-276\slmult0 \up0 \expndtw-3\charscalex100 : FSAINSTEK/Fisika \up0 \expn
dtw-3\charscalex100 : Universitas Airlangga \up0 \expndtw-3\charscalex100 : \up0
\expndtw-3\charscalex100 9 Jam/Minggu\par\pard\ql \li5876\sb0\sl-276\slmult0 \p
ar\pard\ql \li5876\sb0\sl-276\slmult0 \par\pard\ql \li5876\sb0\sl-276\slmult0 \p
ar\pard\ql \li2574\sb276\sl-276\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosup
ersub\cf24\f25\fs24\ul Yopi Cahya\par\pard\ql \li2348\sb1\sl-275\slmult0 \up0 \e
xpndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 NIM 080710276\par\pard\qj \li
20\ri4517\sb275\sl-275\slmult0 \up0 \expndtw-3\charscalex100 : Athoillah \line
\up0 \expndtw-3\charscalex100 : \up0 \expndtw-3\charscalex100 080810658 \par\par
d\sect\sectd\fs24\paperw11900\paperh16820\pard\sb0\sl-240{\bkmkstart Pg14}{\bkmk
end Pg14}\par\pard\ql \li9969\sb0\sl-276\slmult0 \par\pard\ql\li9969\sb155\sl-27
6\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 14\par\pard\
sect\sectd\sbknone\cols2\colno1\colw5716\colsr160\colno2\colw5884\colsr160\ql \l
i3048\sb0\sl-276\slmult0 \par\pard\ql \li3048\sb0\sl-276\slmult0 \par\pard\ql \l
i3048\sb0\sl-276\slmult0 \par\pard\ql \li3048\sb0\sl-276\slmult0 \par\pard\ql \l
i3048\sb193\sl-276\slmult0 \up0 \expndtw0\charscalex100 h.\ul0\nosupersub\cf6\f7
\fs24 \ul0\nosupersub\cf1\f2\fs24 Fakultas/Jurusan\par\pard\ql \li3048\sb1\sl275\slmult0\tx3403 \up0 \expndtw-3\charscalex100 i.\tab \up0 \expndtw-3\charscal
ex100 Perguruan Tinggi\par\pard\ql \li3048\sb1\sl-276\slmult0\tx3403 \up0 \expnd
tw-3\charscalex100 j.\tab \up0 \expndtw-3\charscalex100 Waktu untuk Kegiatan\par
\pard\column \qj \li5876\sb0\sl-275\slmult0 \par\pard\qj \li5876\sb0\sl-275\slmu
lt0 \par\pard\qj \li5876\sb0\sl-275\slmult0 \par\pard\qj \li5876\sb0\sl-275\slmu
lt0 \par\pard\qj \li20\ri3151\sb198\sl-275\slmult0 \up0 \expndtw-3\charscalex100
: FSAINSTEK/Matematika \up0 \expndtw-3\charscalex100 : Universitas Airlangga\pa
r\pard\ql \li20\sb1\sl-276\slmult0 \up0 \expndtw-3\charscalex100 : \up0 \expndtw
-3\charscalex100 9 Jam/Minggu\par\pard\ql \li8204\sb0\sl-276\slmult0 \par\pard\q
l \li8204\sb0\sl-276\slmult0 \par\pard\ql \li8204\sb0\sl-276\slmult0 \par\pard\q
l \li2348\ri1853\sb276\sl-276\slmult0\fi347 \up0 \expndtw-3\charscalex100 \ul0\n
osupersub\cf24\f25\fs24\ul Athoillah \line \up0 \expndtw-3\charscalex100 \ul0\no
supersub\cf1\f2\fs24 NIM 080810658 \par\pard\sect\sectd\sbknone \li2702\sb0\sl-2
76\slmult0\par\pard\li2702\sb0\sl-276\slmult0\par\pard\li2702\sb0\sl-276\slmult0
\par\pard\li2702\sb0\sl-276\slmult0\par\pard\li2702\sb0\sl-276\slmult0\par\pard\
li2702\sb0\sl-276\slmult0\par\pard\li2702\sb276\sl-276\slmult0\fi0 \up0 \expndtw
-3\charscalex100 BIODATA DOSEN PEMBIMBING\par\pard\sect\sectd\sbknone\cols2\coln
o1\colw6225\colsr20\colno2\colw5515\colsr160\ql \li2995\sb1\sl-275\slmult0 \up0
\expndtw-3\charscalex100 1. Nama Lengkap dan Gelar\par\pard\ql \li2995\sb1\sl-2
75\slmult0 \up0 \expndtw-3\charscalex100 2. Golongan Pangkat dan NIP \up0 \exp
ndtw-5\charscalex100 :\par\pard\ql \li2995\sb1\sl-275\slmult0 \up0 \expndtw-3\ch
arscalex100 3. Jabatan Pokok\par\pard\ql \li2995\sb1\sl-276\slmult0 \up0 \expnd
tw-3\charscalex100 4. Fakultas/Program Studi\par\pard\ql \li2995\sb1\sl-276\slm
ult0 \up0 \expndtw-3\charscalex100 5. Perguruan Tinggi\par\pard\ql \li2995\sb1\
sl-275\slmult0 \up0 \expndtw-3\charscalex100 6. Bidang Keahlian\par\pard\ql \li
2995\sb1\sl-275\slmult0 \up0 \expndtw-3\charscalex100 7. Waktu untuk kegiatan P
KM\par\pard\column \qj \li20\ri2657\sb1\sl-275\slmult0\tx119 \up0 \expndtw-3\cha
rscalex100 : Delima Ayu Saraswati, ST \line\tab \up0 \expndtw-3\charscalex100 1

39080807\par\pard\ql \li20\sb1\sl-275\slmult0 \up0 \expndtw-3\charscalex100 : Do
sen\par\pard\qj \li20\ri3064\sb1\sl-276\slmult0 \up0 \expndtw-3\charscalex100 :
FSAINSTEK/Fisika \up0 \expndtw-3\charscalex100 : Universitas Airlangga \up0 \exp
ndtw-3\charscalex100 : Fisika Teknobiomedik \up0 \expndtw-3\charscalex100 : 9 j
am/minggu\par\pard\qj \li7506\sb0\sl-276\slmult0 \par\pard\qj \li7506\sb0\sl-276
\slmult0 \par\pard\qj \li7506\sb0\sl-276\slmult0 \par\pard\qj \li7506\sb0\sl-276
\slmult0 \par\pard\qj \li7506\sb0\sl-276\slmult0 \par\pard\qj \li1281\ri1583\sb2
76\sl-276\slmult0\tx1855 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf24\f25\
fs24\ul Delima Ayu Saraswati, ST \line\ul0\tab \up0 \expndtw-3\charscalex100 \ul
0\nosupersub\cf1\f2\fs24 NIP. 139080807 \par\pard\sect\sectd\fs24}

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{\rtf1{\fonttbl

{\f2 Times New Roman;}

\cf1\f2\fs24 Identifikasi Kadar Antioksidan Dalam Darah dengan Menggunakan

arah. \par\pard\ql \li2275\sb245\sl-276\slmult0 \up0 \expndtw-1\charscalex100 \u

\nosupersub\cf7\f8\fs24 Primary Antioxidants\ul0\nosupersub\cf2\f3\fs24 \ul0\n

\expndtw-3\charscalex100 darah yang disebut {\field{\*\fldinst {HYPERLINK "http

e \up0 \expndtw0\charscalex103 \ul0\nosupersub\cf1\f2\fs24 8,0% (Albumin, globul

p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s

a rentang panjang gelombang tampak yang berkisar \up0 \expndtw-3\charscalex100 a

calex100 \ul0\nosupersub\cf1\f2\fs24 Cuvet spektrofotometer adalah suatu alat ya

\sn geoRight}{\sv 1551}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 553}}

\sn geoRight}{\sv 1551}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 276}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 277}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 11}}

\sn geoRight}{\sv 2684}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 277}}

\sn geoRight}{\sv 500}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 276}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 2717}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 279}}

\sn geoRight}{\sv 1551}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 277}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 276}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 2684}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 276}}

\sn geoRight}{\sv 500}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 277}}

\sn geoRight}{\sv 2717}}{\sp{\sn geoBottom}{\sv 20}}

persub\cf1\f2\fs24 Operasional ampifier (Op-Amp) merupakan rangkaian elektronika

ni 2011. \up0 \expndtw0\charscalex100 Rencana perancangan spektrofotometer secar

w-3\charscalex100 dengan menguji seluruh peralatan. Urutan pengujianya sebagai b

i9969\sb0\sl-276\slmult0 \par\pard\ql\li9969\sb155\sl-276\slmult0 \up0 \expndtw3\charscalex100 \ul0\nosupersub\cf1\f2\fs24 10\par\pard\sect\sectd\sbknone\cols2

keluar \up0 \expndtw0\charscalex104 alat. maka dari hubungan tersebut dibuatkan

\sn geoRight}{\sv 2151}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 276}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 279}}

\sn geoRight}{\sv 711}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 277}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 277}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}

\sn geoRight}{\sv 1496}}{\sp{\sn geoBottom}{\sv 20}}

\sn geoRight}{\sv 20}}{\sp{\sn geoBottom}{\sv 276}}

\sn geoRight}{\sv 10}}{\sp{\sn geoBottom}{\sv 10}}