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Lecture 6 Sample Problem Answers
Lecture 6 Sample Problem Answers
Fall2012(2131)
SampleProblemsLecture6
1. Startingwiththeequilibriumexpressionforoxygenbindingtomyoglobin,derive
thefollowingequation:
=
pO2
(pO
+p
2
50)
Thereactionis:
Mb+O2MbO2
Therefore
=
[MbO2]
[MbO2]+[Mb]
ButIwantthisintermsofoxygenconcentration(pO2)andp50.IknowthatKd=p50,so
IneedtofindKd:
Kd= [Mb][O2]
[MbO2]
orrearrangetogive:
[MbO2]=[Mb][O2]
Kd
Substitutethisvaluefor[MbO2]intotheequationfor,andfactoroutthe[Mb]/K
termtoget:
=
[O2]
[O
]+K
2
d
[O2]=pO2andKd=p50
2. Thep50formyoglobinis2.8torr.Atwhatoxygenconcentrationwillmyoglobinbe
75%saturatedwithoxygen.(Hint:Usetheequationyouderivedinproblem1.)
75%saturatedcorrespondstoaof0.75.Iknowp50(2.8torr)soIwanttosolvefor
pO2when=0.75,rearrangingtheequationabove:
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BIOSC1000
Fall2012(2131)
3. PepsincleavesIgGmoleculesonthecarboxylterminalsideoftheinterchain
disulfidebondsbetweenheavychains.Howmanyphysicalpieceswouldresultfrom
thecleavageofIgGfrompepsin?HowmanyofthepiecesderivefromtheFcregion
ofIgG?
ThreepiecesresultfromthecleavageofIgGbypepsin(seebelow).Onepiececontains
bothFabunits.TheothertwopiecescomefromtheFcregion.
Fab
SS
SS
SS
Pepsincleavage
Fc
4. Thestiffeningofmusclesafterdeath,rigormortis,iscausedbydepletionofcellular
ATP.Describethemolecularbasisofrigor.
Atthetimeofdeath,alargeportionofmyosinheadswillbeboundtoactin.Because
ATPisnecessarytodissociatethemyosinfromactin,theskeletalmuscleislockedin
thecontracted(rigor)stateintheabsenceofATP.
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