Introduction

Milk is a white liquid secreted by the mammary

glands of female cows, buffaloes etc. It is a food of
exceptional inters probability. Milk is the most
nutritious food found in nature. That is why it is
widely consumed both in its natural forms and as
diary
product
like
butter,
cheese
etc.
Milk contains Vitamins, Proteins, Carbohydrates and
the major milk protein casein are found only in milk
and nowhere else in nature. The Composition of
casein in milk is not constant. It depends upon
the Sources of Milk.
Theory
The Protein, Casein, Existent, Milk as the calcium
salt, calcium casein etc. The salt has a complex
structure and form micelles. These micelles have
negative charges on the outer surface.
Requirements

250-ml. beaker, filtrations flask, measuring cylinder,

glass rod, spatula, china dish, dropper, and weight

bore, different samples of milk, 10% acetic acid.
Procedure :
1.Measure 20-ml, of a milk sample with the help of
measuring cylinder and transfer into a clean
250ml. beaker.
2.Make the volume of milk to about 35ml, by
adding water and warm the mixture to about 40
c.
3.Add to the warm mixture drowse and with
constant stirring 10% acetic acid unit the
precipitation is completed.
4.Stir the mixture for another 10 minutes or till the
precipitated casein forms a large amorphous
mass.
5.Filter the casein precipitate.
6.Wash thoroughly with water, dry foils between
folds of filter paper and then in air for 1-2 days
weight the dry solid so obtained.
7.Similarly, determine the amount of casein in
other samples of milk.

Observation

Volume of Milk Taken in each case = 20ml.

Sample
No.
1.
2
3.
4.

Weight of percentage
casein
of casein
SARAS
0.60
3.00%
PANCHAMRAT 0.65
3.25%
AMUL
0.85
4.20%
RATH
0.75
3.70
Quality of Milk

Therefore if an acid is added to milk the negative

charge are neutralized and the neutral protein
precipitated
out.
Ca+2 (casemated)

Results

+2

and

CH3 COOH
(aq)
Casein + (CH3 COO)2 Ca
Discussion

Different samples of milk contain different percent of

casein and the casein contents in maul brand are
appreciable. At present amul is the best brand of
milk available in the market.