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MicroorganismEndorsement

TheIllinoisJuniorAcademyofScience

TheseruleswillbestrictlyenforcedfortheStateScienceExposition.
NoregionshouldsendaprojecttotheStateExpositionthatdoesnotmeettheseregulations.
Studentsandsponsorsdoingamicroorganismprojectmustcompletethisform.Thesignatureofthestudentorstudentsand
thesponsorindicatesthattheprojectwasdonewithintheserulesandregulations.Failuretocomplywiththeseruleswill
meanthedisqualificationoftheprojectatthestatelevel.ThisformmustfollowtheSafetySheetintheprojectpaperandon
theprojectboard.
1
.
Thisareaofsciencemayinvolvemanydangersandhazardswhileexperimenting.Itisthesoleresponsibilityofallteacher(s)/sponsor(s)toteachstudentsproper
safetymethodsandsteriletechniques.
2.TheIllinoisJuniorAcademyofScienceprohibitstheuseofprimaryorsecondaryculturestakenfromhumansorothervertebrateanimalsinanyprojectbecause
ofthedangerfromunknownvirusesorotherdiseasecausingagentsthatmaybepresent.Pureculturesofmicroorganismsknowntoinhabitvertebrateanimalsmay
beobtainedfromreputablesuppliersandusedinpropersettings.
3.Microorganismexperimentsmustbeconductedinalaboratorysuchasscienceclassroomorresearchfacility.
4.ProjectsinvolvingvirusesandrecombinantDNAshouldbedonewiththehelpofaprofessionalandshouldcomplywiththeNationalInstitutesofHealth(NIH)
Guidelinesunlesstheprojectislimitedtoakitobtainedfromalegitimatesupplyhouse.
5.AllculturesshouldbedestroyedbymethodssuchasautoclavingorwithasuitableNaOCl(bleach)solutionbeforedisposal.

Completeallboxesofthefollowingchart.
Genusandspeciesof
organism(s)beingused.

PleurotusOstreatus

Nameofthereputable

sourceoftheorganism(s)
FungiPerfecti
beingused.

Methodofdisposalof
ThemicroorganismswiththesawdustmixturewillbesenttoaEPAdisposalunitsincetheywill
theorganism(s)beingused. containPCBcontaminantremnants.
Listthelocationwherethe NilesNorthSTEMlab
labworkwasconducted.
Describetheuseof
PCBtoxinswereaddedtoasawdustmixturecontainingthismicroorganism,totest
microorganismsinthis
whetherfungalbiodegradationwasafeasiblemethodofremovingPCBtoxins.
project.
Otherprecautionstaken
Thismicroorganismwasfrozenintherefrigeratorbeforeuse,andwasfrozenafteruse
toensuremicroorganisms untiltimefordisposalcame.
areusedsafelyinthis

investigation.

Thesignaturesofthestudentorstudentsandsponsorbelowindicatethattheprojectconformstotheaboverulesofthe
IllinoisJuniorAcademyofScience.

(Sponsor)*

(Date)

(Student)

(Student)

*
Asasponsor,Iassumeallresponsibilitiesrelatedtothisproject.
ThisSheetMustBeTyped
Thisform
must
bedisplayedonthefrontoftheexhibitorsdisplayboard.Itmaybereducedtohalfasheetofpaper
8.5inches(vertical)X5.5inches(horizontal).

Checkboxifexception/approvalletterfromaninstitutionwhereresearchwasdone,ortheIJASSRCisrequiredandattached.

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