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Ligand Binding and Interactions
Ligand Binding and Interactions
Name____Key______________
t1/2 = 0.693/
= 0.693/0.00675
= 102 minutes or if you solved in hours, = 1.7 hours
-emitter
solvent in
excited state
fluor that
absorbs energy from
solvent and emits
fluorescent signal
Ligand Binding:
4. (1pt) Consider the following ligand binding interaction:
L+M
LM
v/[L]
1.000
0.500
0.000
20
0
20
40
60
80
100
v
5. (1 pt) What does the value of the x-intercept mean? the value of n = total
binding sites
6. (2 pts) How would you determine the formation constant for the binding
interaction shown in the Scatchard plot? (What constant from the plot is
equivalent to the formation constant?)
How does this relate to the
dissociation constant?
the slope is equal to -Kf
y intercept = nKf
Kf = 1/ Kd
7. (1 pt) What information does a Hill plot tell you?
it tells you whether you have cooperative binding and whether it is positive or
negative cooperativity