Biotech 1: Lab Practical Exam Study Guide

The list below contains topics that may/will be tested on the lab practical exam.
Use your class notes and other resources to prepare for the exam.
You will be given 3:00 at each station to complete all work. Complex stations will
have a rest area next to them to allow for extra time.

1.) Metric Conversion

2.) Mass/Volume Solution Prep
3.) %Mass/Volume Solution Prep
4.) Molar Solution Prep
5.) Dilutions
6.) Restriction Enzymes
7.) Micropipette Operation
8.) Serological Pipette Operation
9.) Centrifuge Operation
10.)
Spectrophotometers and Solution Concentration
11.)
Bacterial Transformation
12.)
Streak Plates and Spread Plates
13.)
Aseptic Technique
14.)
DNA extraction techniques
15.)
Polymerase Chain Reaction
16.)
Biological Indicators
17.)
Standard Curves
18.)
Experimental Controls
19.)
Gel Electrophoresis and interpretation of Agarose Gels
20.)
Antibiotic Sensitivity Test of Bacteria
21.)
Hydrophobic Interaction Chromatography
22.)
Polymerase Chain Reaction