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Enzymeinquirylabsheets 1
Enzymeinquirylabsheets 1
Procedure:
1. Retrieve 8 test tubes.
2. In the first test tube add 2 mL of glucose solution
(positive control).
3. In the second test tube add 2 mL of whole milk (control).
4. In the third test tube add 2 mL of fat free milk (control).
5. In the fourth test tube add 1 mL of lactase (negative
control).
6. In the fifth test tube add 1 mL of lactase and 2 mL of
whole milk mix at room temperature.
7. In the sixth test tube add 1 mL of lactase and 2 mL of fat
free milk mix at room temperature.
8. In the seventh test tube add 1 mL of lactase and 2 mL of
lactose (positive control).
9. Let solution sit for 3 minutes.
10.
Take the temperature of all 8 solutions and record.
11.
Test each solution for glucose by dipping paper into
the solution.
12.
Test each solution for protein by dipping paper into
the solution.
13.
Test each solution for pH by dipping paper into the
solution.
14.
Record initial color of paper.
15.
Let each paper sit for 2 minutes.
16.
Record final color of paper.
17.
Gather new test tubes with solutions of test tubes 7
and 8.
18.
Heat each solution on a hot plate in boiling water
100 degrees C.
19.
20.
21.
22.
23.
24.
and 8.
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Contents Final
Glucose
Levels
Presence of lactase
enzyme
Temperature of
Solution
glucose
++
room
whole
milk
room
fat free
milk
room
lactase
room
lactase
and
whole
milk
++
room
lactase
+++
and fat
free milk
room
test
tube
contents
final glucose
levels
presence of
lactase enzyme
temperatur
e of
solution
glucose
++
boiling 100
degrees C
whole
milk
boiling 100
degrees C
fat free
milk
boiling 100
degrees C
lactase
boiling 100
degrees C
lactase
and
whole
milk
boiling 100
degrees C
lactase
and fat
free milk
boiling 100
degrees C
Lactase at 0 degrees C
test
tube
contents
final glucose
levels
presence of
lactase enzyme
temperatur
e of
solution
glucose
++
30 degrees
C
whole
milk
30 degrees
C
fat free
milk
30 degrees
C
lactase
30 degrees
C
lactase
and
whole
milk
30 degrees
C
lactase
and fat
free milk
30 degrees
C
Photos
Room temperature
Boiling
Cold
solutions
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