BOI207

GENERAL MICROBIOLOGY
PRACTICAL 3: ASEPTIC
TECHNIQUE
PRACTICAL 4: PURE CULTURE
TECHNIQUE

NAME: SALIHAH BINTI ZAKARIA

STUDENT ID: 126197
GROUP: 14

PRACTICAL 3: ASEPTIC TECHNIQUE

OBSERVATIONS
A- Broth to broth

B- Slant to broth

C- Broth to plate
QUESTIONS
1. Yes, something grow in all of my transfer. Both of the broth turn
cloudy while the plate show E. coli grow on the plate after been
transferred for one day.
2. Yes, my agar plate appear to be contaminated. There was a presence
of white colonies outside of the streaking line.

3. If in the broth cultures have floaties or showing other color than the
cloudy, the broth would had contaminated with a microbe other than the
one that wanted to grow.

PRACTICAL 4: PURE CULTURE TECHNIQUE

OBSEEVATIONS AND QUESTIONS

1. Streak plate method
Clear isolation of red S. marcescens
and white E. coli

2. Pour plate method

Pour plate 2.
Growing on the medium (surface
colonies) - red S. marcescens
Growing within the medium
(subsurface colonies) - E. coli

3. Streak plate method showed the best separation of species.

4. E. coli able to grow both on the surface and within the medium.
Colonies are the larger at the location 1. Because the early step, the
microorganisms still not separated from each other before being thin out.
5. Colony is a grouping of individuals of the same species rising from a
single bacteria cell. Colonies are genetically identical as they are formed
from a single cell that replicates and grows outward.
6. The advantages of streak-plate method:

The original culture is diluted serially and a small volume of the

final dilution is spread on the surface of an agar plate. A dilution
gradient is established across the face of the petri plate as bacterial
cells are deposited on the agar surface.
Spread millions of cells over the surface.
Individual cells deposited at a distance from all others;
Divide forming distinct colonies.
Distinct colonies do not touch any other colonies;

7. Agar must first be cooled to 50oC to prevent condensation which

could cause moisture on the agar surface allowing for the organisms to
spread over the surface resulting in a faulty isolation process
8. 2 reasons the plate should be inverted during incubation:
We label the bottom surface of the agar plate. It is easier to find the
plate when it is inverted.
To reduce possible condensation on the agar surface