α − incomplete form of hemolysis

β - completer destruction of RBC

y – indicates the absence of hemolyisis around the colony.
Regi. Streptoococcal
Media PathogensResult
Procedure 20, March.
Comme
2010 nts
Organism: S. Enterococcus S. bovis S. salivarius Pneumonia
pyogene Faecalis S.mitis
Enterococcus nonenterococcus
Ser Groups:
Test: A D K,H,N (viridans)
Determines Staph. from Strep.
Catalase
Refer to Staph. Graph for procedure

β α (ω ε α α y α Medium indicates
• ethyl κ) S. pyogene a degrees of
Hemolysis alcohol 1.Inoculate Gram + hemolysis by
bacterial enzymes
• Blood agar
2.Streak jab of the red blood
plate • greenish-
technique – to cells within the
• (TBS) x3 gray in color
medium;
create growth • medium
surface/subsurf. total hemolysis
around the (β) is seen as a
S. bacterial clear halo around
3.Subculture each
into tryptic soy colonies colonies

α broth (TSB) as
a working stock.
has lost its
color
partial hemolysis
(α) is seen as a
green halo and
no hemolysis (γ)
is seen as no
β change in the
medium

S R R R R
• Blood agar 1.Streak (heavily) Not looking for Disks saturated
Bacitrican plate a blood agar plate isolated with a Bacitracin
• (ABT) disc with the isolated colonies… will inhibit strains
Sensitivity colony of beta- Look for: of Group A
hemolytic Zone of Lancefield
S.pyogene inhibition

- + + - -
Bile Salt Tolerance 1.Label and No dark brown S. pyogenes does
Esculin Broth Inoculate one tube complex is not hydrolyze
hydrolysis (6.5% NaCl) of 6.5% NaCl formed. This is esculin in the
a negative presence of bile.
2. Label and result.
Inoculate one slant No dark brown
Rapid routine of Bile Esculin complex is
More than half
identification agar with a formed. This is a
the medium
of gram- (single) streak negative result.
dark brown.
positive cocci Enterococcus
This is a
3. Incubate the positive result. faecalis
Determine tubes for 24 hours. hydrolyzes esculin
organism When enterococci in the presence of
ability to: hydrolyze the bile and turns
hydrolyze esculin, a black more than half the
glycoside pigment forms in medium dark
the medium brown. This is a
Streptoococcal Pathogens 20, March.
2010