Staphylococcol Pathogens 2010

S. aureus S. epidermidis S. sapropphticus

Test
Regiment Media Procedure Result Comments
1.On slide drop
3% (H2O2) (H2O2) + Bubbles Bubbles
Hydrogen Positive 2.Mix small Formation =
Catalase amt. of
Peroxide Indicates All Staph.
organism production of Staph.
3.Incubate 24- free O2 Staph.
48 hours
1. MSA plate S. aureus S. aureus
Positive 7.5% NaCl Ferments
MSA (inhibits) 2.Single line Yellow Halo the manital
inoculation in media Gram +
S. aureus m-staph All Staph.
broth 3. Incubate + Staph.
Staph.
1.DNA agar S. aureus S. aureus
plate Produces
DNase agar Clear Colorless hydrolytic
DNA plate 2.Single line Halo enzyme DNase
Positive Inoculation
Methylene +
green 3.Incubate
S. aureus S.epidermidis
S.saprophyticu
1.Inoculate Sensitive Distinguishes
Mueller- Mueller Hinton S. aureus S. edidermidis
Novobioci Hinton agar plate S.epidermidi from
n agar plate >17 mm to S.saprophyticu
Sensitivity 2.Apply antibiotic is
Novobiomic antibiotic disc determined by Resistant
antibiotic disc Kirby-Bauer
3.Incubate method
S.aureus S. epidermidis S. saprophyticus
Colonial Golden Yellow
Pigment
No Zone of Inhibition Zone of Inhibition