Quality control/assurance

assignment
SOP of calibration of a pH meter
Calibration and adjustment
These should always be performed after:
switching on the pH meter
replacement of electrode
checking the calibration and the deviation of the pH from
the theoretical value of the standard buffer appears to exceed
0.05 unit.

When the pH meter is on and already adjusted then only a

check of the adjustment is needed (described in Section 7.1
of this SOP),
Calibration step 1
Transfer sufficient standard buffer solution pH 7.00 to a 50 ml or
100 ml beaker.
Measure temperature of buffer and set switch 14 (temp.
compensation) to this temperature.
Immerse electrode in buffer solution and push button 4
(measure).
With button 6 (Ucomp) adjust value on display (8) to theoretical
pH value of the buffer at the measured temperature. (Note: this
value can be read from a table enclosed with the standard
ampoule).
Push button 3 (stand-by). Rinse electrode with water. Setting of
switch 6 (Ucomp) should now not be changed any more
Calibration step 2
Transfer a sufficient volume of one of the two other buffer
solutions (pH 4 or 9) to a 50 ml or 100 ml beaker. (Note: this
second buffer is chosen such that the pH of the solution to be
measured falls in between , the first and second calibration
buffer).
Measure temperature of buffer and adjust switch 14 (temp.
compensation) to this temperature.
Immerse electrode in buffer solution and push button 4
(measure),
With switch 13 (slope) adjust the value on the display to the
theoretical pH value of this buffer. (Note: this value can be read
from a table enclosed with the standard ampoule).
Calibration step 2
The setting of switch 13 may not be lower than 0.95. If
this condition is not met, this electrode may not be
used for the measurement and must be exchanged for
another one which does meet the condition.
Push button 3 (stand-by) and rinse electrode with
water.
As a check, repeat readings of buffers (pH 7 first) and
readjust according to Step 1 and 2 if necessary.
Reference:
http://www.fao.org/docrep/W7295E/w7295e07.htm#a
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%20meter%20metrohm%20e%20632