GbioSciences

GbioSciences

0 Item(s) in my Cart
Cart Amount $0.00
SEARCH

GO

• Home

• Products

• Literature

•
• Research Products

GO
Email Password Forgot Password? | Register Here

Zymogram: Study of an Active Enzyme with Electrophoresis

• Protocol MSDS Certificate of Analysis

• Catalog
• Description
• Size
• Price
• Action
• Shipping Condition

• Protocol

• BE-420
• Zymogram: Study of an Active Enzyme with Electrophoresis
• 6 groups of 4 students
• $103.00
•
• Ambient

•
Zymography is an electrophoretic technique that includes a substrate copolymerised with the polyacrylamide gel for the detection of enzymes
and their activity. Samples are prepared without denaturing the active enzymes present in the samples. Following electrophoresis, the gel is
placed in an enzyme activation buffer which allows the enzymes present in the sample to become active and digest the substrates
copolymerised in the gel. The zymogram is subsequently stained and the areas of enzyme activy and digestion become visible.
Gelatin is the most commonly used substrate, and is useful for demonstrating the activity of gelatin-degrading proteases, but zymography has
been applied to a variety of enzymes, including xylanases, proteases, lipases, etc. Suitable for drug discovery and screening of low abundunt
enzymes and their isomers in complex mixtures.

Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s
Guide and separate Student’s Guides.
FEATURES & APPLICATIONS

This lab activity teaches the following:

 o A modified protein electrophoresis technique.
o Use of electrophoresis to study enzyme activity.
o Introduction to zymograms.

ADDITIONAL HARDWARE

o Protein electrophoresis equipment.

• General Biotechnology

• Microbial Studies

• Molecular and Genomic Studies

• Protein and Proteomic Studies

• Immunotechnology Studies

• Miscellaneous Biotechniques

• Chemicals, Buffers & Reagents

• Laboratory Equipment

• Training & Workshops

Newsletter
Enter email address to receive monthly newsletter and discounts

GO

Home | Careers | SiteMap | News | Distributors | About Us | Contact Us | Help

© 2008 Geno Technology Inc., USA. All Rights Reserved.