pCHAC MCS1-IRES-HyrgoR Expression

Vector
Cat#: ABP-PVL-IRES10H
ABP-PVL-IRES10HL

R etroviral infection of cells is one of the most efficient tools used to in-
troduce genes into most dividing mammalian cells, including human.
pCHAC MCS-IRES-HygroR is a Moloney Murine Leukemia Virus (MMLV)
based retroviral vector, which routinely results in a titer of 108 TU/ml or
Vector Properties greater. The combination of the multiple cloning site (with approximately
421-1012 5’ LTR six restriction sties) and IRES allow for the production of multiple proteins
from a single vector. An ampicilin resistance gene is added to further in-
2307-2350 MCS crease the specificity and efficiency of the vector.

2351-2929 IRES

2942-3967 Hygromycin Resis-

tance Gene
4080-4671 3’ LTR

4946-5585 pUC Origin

5735-6595 Ampicilin Resis-

tance Gene

S afety issues
Retroviral vectors should be han-
dled using NIH BSL-2 safety guide-
lines. For more information, please
see Biosafety in Microbiological and
Biomedical Laboratories <4th edition>
which is available on the Web sites
of the National Institutes of Health at
http://bmbl.od.nih.gov.

F or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer
this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted
use without the express written authorization of Allele Biotech is strictly prohibited

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