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  • GM Micro Present A Ion Sat

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    reirei08
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    The document describes genetically modifying three different bacteria - E. coli Nissle 1917, Pseudomonas flourescens HK44, and Acetobacter xylinum. For E. coli, the cqsA gene was inserted using the pKD3 vector and pKD46 ligase, and transformants were selected for antibiotic resistance. For P. flourescens, the lux gene was inserted using pUTK21 vector and ligase, and transformants were screened under nitrogen and phosphorus starvation. For A. xylinum, the IS1031 element was inserted using pAXC145 vector and ligase, and transformants were selected for antibiotic resistance.

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    GM Micro Present a Ion Sat

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    The document describes genetically modifying three different bacteria - E. coli Nissle 1917, Pseudomonas flourescens HK44, and Acetobacter xylinum. For E. coli, the cqsA gene was inserted using the pKD3 vector and pKD46 ligase, and transformants were selected for antibiotic resistance. For P. flourescens, the lux gene was inserted using pUTK21 vector and ligase, and transformants were screened under nitrogen and phosphorus starvation. For A. xylinum, the IS1031 element was inserted using pAXC145 vector and ligase, and transformants were selected for antibiotic resistance.

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    Download as PPTX, PDF, TXT or read online from Scribd
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    16 views3 pages

    GM Micro Present A Ion Sat

    Uploaded by

    reirei08
    The document describes genetically modifying three different bacteria - E. coli Nissle 1917, Pseudomonas flourescens HK44, and Acetobacter xylinum. For E. coli, the cqsA gene was inserted using the pKD3 vector and pKD46 ligase, and transformants were selected for antibiotic resistance. For P. flourescens, the lux gene was inserted using pUTK21 vector and ligase, and transformants were screened under nitrogen and phosphorus starvation. For A. xylinum, the IS1031 element was inserted using pAXC145 vector and ligase, and transformants were selected for antibiotic resistance.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    Download as pptx, pdf, or txt
    of 3

    R.E.

    cqsA Gene
    pKD3
    Ligase
    pKD46

    Recombinant vector

    Transformation: CaCl

    Screening : Antibiotic Resistance

    GM E. coli Nissle 1917


    R.E.

    Lux Gene
    Ligase
    pUTK21

    Recombinant vector

    Transformation: Electroporation

    Screening: Nitrogen &


    Phosphorus starvation

    GM Pseudomonas flourescens HK44


    R.E.
    (IS) element, IS1031
    pAXC145. Ligase

    Recombinant vector

    Transformation: CaCl

    Screening : Antibiotic Resistance

    GM Acetobacter xylinum

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