The Effects of Ephedrine

on the Lumbriculus
variegatus
Taylor Cecil, Chris Darlington,
and Paige Phillips
HCOP-PE 2008
Ephedrine Background
 Hypotheses
• We hypothesize that by
administering the Lumbriculus
variegatus with ephedrine, the
pulsation rate will rise.
• We hypothesize that the Lumbriculus
variegatus administered with
ephedrine will regenerate too
quickly, only to ultimately die as a
result of too much rapid growth.
Pulsation Experimental
Design
Regeneration Rate Design
 Observations

Lab Video of pulsation rates.

Lab Video of regeneration testing.
 Materials and Methods:
• Pulsations
Control Group= 8 worms placed
individually in 2.5 mL of spring water.
• Treatment Group= 8 worms placed
individually in .5 mL of 1% ephedrine
solution.
• Observed each group for 10 minutes, then
for 20 minutes.
• Removed worms individually and placed
them under a microscope to determine
number of pulsations/ minute.
• Mean and standard deviation were
calculated, and analysis of variance was
Pulsation Rates
 Materials and Methods:
Regeneration
• Control group-20 dissected worms were
placed in 2.5 mL of spring water.
• 1st treatment – 20 dissected worms were
placed in 2.5 mL of 1% Ephedrine solution.
• 2nd treatment- 20 dissected worms were
placed in 2.5 mL of .1% Ephedrine
solution.
• After dissecting the worms, they
regenerated in the dark for seven days,
and then the new growth of the worms
was counted.
Regeneration Rates