Scribd Logo
Upload

Welcome to Scribd!

  • Practice 3 RFLP

    Uploaded by

    Janani Sridhar
    16 views7 pages
    RFLP, or Restriction Fragment Length Polymorphism, is a technique that involves digesting DNA with restriction enzymes, which cut DNA at specific sequences, and analyzing differences in the lengths of fragments produced, revealing genetic variation. Restriction enzymes cut DNA into fragments of varying sizes, which are then separated by electrophoresis. If a sequence difference creates or disturbs a restriction site, fragments of different sizes will be produced, revealing polymorphisms.

    Original Description:

    Original Title

    Practice 3 Rflp

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PPT, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    RFLP, or Restriction Fragment Length Polymorphism, is a technique that involves digesting DNA with restriction enzymes, which cut DNA at specific sequences, and analyzing differences in the lengths of fragments produced, revealing genetic variation. Restriction enzymes cut DNA into fragments of varying sizes, which are then separated by electrophoresis. If a sequence difference creates or disturbs a restriction site, fragments of different sizes will be produced, revealing polymorphisms.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    16 views7 pages

    Practice 3 RFLP

    Uploaded by

    Janani Sridhar
    RFLP, or Restriction Fragment Length Polymorphism, is a technique that involves digesting DNA with restriction enzymes, which cut DNA at specific sequences, and analyzing differences in the lengths of fragments produced, revealing genetic variation. Restriction enzymes cut DNA into fragments of varying sizes, which are then separated by electrophoresis. If a sequence difference creates or disturbs a restriction site, fragments of different sizes will be produced, revealing polymorphisms.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    of 7

    RFLP

    Restriction
    Restriction Fragment
    Fragment
    Length
    Length Polymorphism
    Polymorphism
    Marie Černá,
    Markéta Čimburová,
    Marianna Romžová
    RFLP –Restriction Fragment
    Length Polymorphism

    restriction analysis of DNA


    by its digestion
    with restriction endonucleases (RE)
    in specific restriction sites
    RFLP –Restriction Fragment
    Length Polymorphism

    in the case the sequence difference


    (polymorphism) creates or disturbs
    a specific site for RE,
    after restriction, fragments with
    different sizes are formed
    Principle
    • starting DNA
    (genomic DNA, PCR product)

    • digestion with a restriction enzyme


    into fragments with different sizes

    • fragments electrophoresis separation


    Restriction endonucleases
    (RE)

    • known about 2100 bacterial RE

    • RE recognize variously short


    nucleotides sequences (4,6,8),
    in which then they digest covalent
    phosphodiester bonds
    ???

    • Do RE often digest, which recognize


    shorter or longer sequences?

    • What is a role of RE in natural systems?

    You might also like