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Biology Lab Practicalomgomg
Biology Lab Practicalomgomg
CELL STRUCTURE
Objectives:
Before you leave at the end of the laboratory period you should know the parts of a microscope and how to
determine total magnification. You should be able to recognize the organelles in a plant and an animal cell, and
recall the functions of each. You should also know the stains that you used and what they stained.
Introduction:
As you will find in this course, there is no such thing as a "typical" cell. During this period you will look at
four "representative" eukaryotic cells: two plant cells (one with chloroplasts that are easy to focus on, and one
without chloroplasts that will be more challenging), one animal cell, and one example of a single-celled organism.
In each case you will try to locate as many organelles as you can. You will also examine a prepared slide of some
prokaryotic bacterial cells. Your textbook is an excellent reference, so you may want to refer to it during this
exercise.
I. MICROSCOPES
Your goal in this section is to learn the names of all of the parts of the microscope.
B. Magnification
The magnifying power of the ocular and objective lenses used
You can magnify the image and move around to see the
magnification. It is often referred to as the scanning lens and should be used first when viewing a
new specimen. On this microscope the scanning lens has a red band around it to make it easy to
identify.
• The next highest magnification is the 10 X lens also called the low power lens. It has a yellow band.
• The highest power dry lens is the 40 X lens. It has a blue band.
Some microscopes will have additional higher power objective lenses (for example 100 X). These lenses
require that a drop of immersion oil be placed between the lens and the specimen.
Now that we know the magnifying power of the ocular and objective lenses, we can calculate the total
• To calculate the total magnification, multiply the power of the ocular lens times the power of the
C. Field of View
Sometimes it is necessary to determine the size of an object that you are viewing under the microscope.
There is an easy way for you to estimate size. If you know the diameter of the field you are seeing in the
microscope, you can estimate the size of the object you are viewing.
For example:
Here you see the same object with increasing magnification. At the lowest magnification the object
diameter is at the different magnifications and determine the approximate size of the object you are
viewing. In fact, you can buy a ruler mounted on a microscope slide that is especially designed for
this.
You will do this more simply by placing a piece of graph paper on a microscope slide and viewing it under
the microscope. Each box on the paper is a 1 mm square, but for microscopy, millimeters are too large of a unit of
1 mm = 1000 micrometers
Let's try this at our lowest magnification using the scanning lens (40 X total magnification).
Now estimate the field diameter in micrometers.
Now do the same using the 10 X ocular and the 10 X low power objective lens at a total magnification of
As you can see, each time the magnification is increased, the block on the graph paper gets larger.
If we try to estimate field size using graph paper with the 40 X objective, the block will be so large that we
will not be able see the lines clearly and it will be difficult, if not impossible, to estimate the field size.
We know that the higher the magnification is, the smaller the field diameter is. Therefore, the field
diameter is inversely proportional to the magnification. We can use a mathematical formula to estimate the field
size at 40 X.
Let's do this using the numbers we just calculated for the 10 X objective. Remember, the field diameter
with the 10 X objective lens was 1700 micrometers. Now fill in the numbers in the equation.
Now solve the equation for the field diameter of the high magnification lens, you get the .
D. Image Formation
When a microscope magnifies an image it shifts the orientation of the object you are viewing.
For example, if you cut out the letter "e" from a newspaper and put it under the microscope what happens
right?
To simulate this, place your cursor on the letter "e". Hold down the left mouse button and slide the cursor
to the right.
Place your cursor on the letter "e". Hold down the left mouse button and slide the cursor upward.
What happened?
II. Cells
A. Plant Cells
1. Elodea: leaf cells
Elodea is a decorative aquatic plant often found in fish tanks. A small leaf has been removed from the
plant and placed with the lower surface down in a drop of water on a microscope slide. If you examine the leaf
using the scanning lens you will find the midrib running down the center of the leaf. The midrib contains the main
Now turn to higher magnification and examine a portion of the leaf away from the midrib. Can you identify
the chloroplasts in the upper photo and the cell walls in the lower photo?
Click here to confirm your answers..
Remember that a cell, such as this leaf cell, is a three-dimensional structure. The cell wall surrounds the
cell on all six sides. The chloroplasts and other organelles are held against the sides of the cell by the large central
vacuole.
You can confirm the 3 dimensional nature of the cell by focusing up and down on while observing one cell.
Try this.
The cytoplasm of the cell is not static but "streams" around the perimeter of the cell. The chloroplasts are
and make them easier to see under your microscope. This portion of the lab is not done in the virtual laboratory.
2. Onion: Epidermis
A small piece of epidermis from the scale of an onion bulb has been removed and a a wet mount has been
prepared for microscopic examination. If you are not experienced with the microscope, you may be able to see
little besides the cell walls which appear as little boxes. If you reduce the amount of light by using the condenser
diaphragm, you may be able to see the nucleus, cytoplasm, and vacuole.
Look at the onion preparation with the condenser diaphragm wide open. Now place the cursor over the
image of the onion and close the diaphragm. What happens to the contrast?
With the diaphragm closed you can easily see the onion epidermal cells. Now determine the size of a single cell
of the cell?
B. An Animal Cell
You will use a cheek cell (simple squamous epithelium) as an example of an animal cell. A small amount
of material has been gently scraped from the inside of a mouth and mixed with a drop of water on a glass slide. A
cover slip was then added. What do you see under the microscope?
The image has low contrast. You can increase the contrast by closing down the diaphragm. When the
diaphragm is in the closed position, you will have maximum contrast. When it is in the fully open position, you
have maximum resolution but the image has very low contrast. Most of the time, we compromise and close the
diaphragm about half way to maximize resolution and contrast as much as possible.
C. Euglena
a single-celled eukaryote
Euglena is a single-celled eukaryotic organism that is often called a "plant-like" organism since it is
photosynthetic.
Now look at a single Euglena cell. What organelles can you see in the cell?
Flagellum –
Nucleus –
Eyespot –
Chloroplast –
D. Bacteria
single-celled prokaryotes
Bacteria are extremely small and often are not visible with the 10 X objective lens. The prepared slide of
The round bacteria are often clustered in groups of two, four or more cells.
Now find the rod-shaped bacteria. Notice that not all of the rods are of the same length.
Now find the spiral-shaped bacteria.
Mitosis is the sequence of events by which the nuclear material of one cell is distributed, by a process
At the right is a longitudinal section through an onion (Allium) root tip. The root tip is responsible for the
downward growth of the root and therefore, is one of the regions in the plant where cells are actively dividing and
elongating. Because of this, the root tip is an excellent system in which to study the process of cell division
(cytokinesis)and nuclear division (mitosis) Furthermore, the chromosomes are fairly large and distinct, and this
Part 1.
b.What differences can you see when you compare the nucleus of a
Part 2.
Cell Structure 30/11/2009
a. Review the diagrammatic summary of cell division in your textbook before you begin.
Part 3.
Microscopy Lab Now that you have seen how nuclei divide, you can begin the microscope exercises.
Interphase - At interphase the nuclear envelope is still intact, the nucleolus is present and
Prophase - During prophase the nuclear envelope disappears, the nucleolus is gone and
Metaphase – During metaphase the chromosomes are distinct and line up near the center of the
cell.
Cell Structure 30/11/2009
Anaphase - During anaphase the chromatids are separated and the two groups of chromosomes
and are positioned at opposite sides of the cell. The nuclear envelope begins to reform.
When you have identified at least one cell in each stage, proceed to the next section.
Part 4.
Click to begin
Slides of whitefish blastulae will be used to show mitosis and cell division in animal cells.
Cell Structure 30/11/2009
Although the result of these processes and many of the events are the same or very similar to that of the
plant cells, there are some differences. See what differences you can detect.
Place the cursor over a dividing cell and click once. Identify the stage of division.
Interphase - At interphase the nuclear envelope is still intact, the nucleolus is present and
Prophase - During prophase the nuclear envelope disappears, the nucleolus is gone and
Metaphase – During metaphase the chromosomes are distinct and line up near the center of the
cell.
Cell Structure 30/11/2009
Anaphase - During anaphase the chromatids are separated and the two groups of chromosomes
and are positioned at opposite sides of the cell. The nuclear envelope begins to reform.
Cell Structure 30/11/2009
Reproduction: Protists and Fungi 30/11/2009
Objectives:
• To examine some of the structural variation found in the simpler organisms on earth,
• To understand how the relative position of meiosis and fertilization varies in different life
cycles.
This capacity may also be observed in some sexual cycles, as in the mushrooms.
Before you leave at the end of the laboratory period you should be able to tell a plant-like protist (alga)
from a fungus, and specify the kingdom of each. You should know how to recognize the haploid and diploid (and
dikaryotic) phases of each organism studied, and what cells were formed by mitosis and by meiosis.
Introduction:
Most plant and animal cells are diploid (2n), meaning that they contain two complete sets of their genetic
materials, located on two complete sets of chromosomes. Alternately, some plant and animal cells are haploid
(1n), containing only one complete set. [You may want to review the discussion of these terms in Lab 2.]
Cell Reproduction 30/11/2009
Dependent upon the organism, both diploid and haploid cells can divide by mitosis. In both cases, each
"daughter cell" has the same amount of genetic material (chromosome number) as the "mother cell".
During a second type of cell division called meiosis, a diploid cell undergoes a "reduction division" to form
four haploid cells. (The reason that four cells are formed will be covered later in the semester.)
The diploid number is restored when two haploid cells fuse during fertilization.
Cell Reproduction 30/11/2009
The actual location of meiosis and fertilization in a sexual cycle depends upon the organism. For example,
the only haploid cells produced by humans (and other animals as well) are the sex cells (gametes). The gametes,
egg and sperm, are formed by meiosis; they immediately combine to restore the diploid condition by fertilization,
forming the zygote. The mature adult is then formed by a large number of mitotic divisions. Therefore, the relative
number of haploid cells is small, as indicated in the sexual cycle to the right.
On the other hand, many protists have a sexual cycle where the only diploid cell is the zygote. This
zygote immediately undergoes meiosis upon "germination"; all of the other cells are formed by mitosis - including
These two cycles can be abbreviated by simply showing meiosis and fertilization as gray arrows. On the
right, the only diploid cell is the zygote; on the left, the only haploid cells are the gametes. To help you keep track
of which cycle goes with each organism you will study in this lab, a simplified cycle will be found at the upper right
In some instances the "many haploid cells" remain separate (e.g., Chlamydomonas), in other cases the
cells are attached to form a filament (e.g., Oedogoniium and Spyrogyra), and in still other cases the cells actually
form a multicellular organism (not seen in lab, but described in your textbook).
Cell Reproduction 30/11/2009
Plants (and some larger plant-like protists) have a third sexual cycle in which both diploid and haploid
phases are multicellular, resulting in an "alternation of generations". We will study this cycle in the next two
laboratories.
Finally, the alternation of meiosis and fertilization, and the resultant recombination of genes, is associated
with the sexual cycle. Many organisms also have an asexual cycle where the offspring are simply produced by
mitosis and therefore are genetically identical to the parent (in other words, they are "clones"). This can be an
asexual reproduction of diploid organisms, as in the propagation of some commercial plants. It can be an asexual
reproduction of the haploid organisms, as the production of spores by many fungi. And some organisms, such as
Most biologists today subdivide all of life on earth into six kingdoms. The prokaryotic organisms, such as
the bacteria you observed in Laboratory 1, are in Kingdom Eubacteria and Kingdom Archaebacteria. Many if not all
of the simpler plant-like organisms ("algae") are placed with simpler animal-like organisms into a Kingdom
Protista; they are therefore called protists. The fungi that you will observe today are given their own kingdom.
Although the term "algae" is no longer used in classification, it is still a useful term to describe aquatic
photosynthetic organisms with little tissue differentiation. Chlamydomonas is a single-celled protist; Spirogyra and
Oedogonium are filamentous. Fucus is a macroscopic organism, placed in the plant kingdom in some textbooks.
Cell Reproduction 30/11/2009
• A. Single-celled protists
Cell Reproduction 30/11/2009
1. Euglena
hints
Note that these cells divide by mitosis, and therefore asexually. Does Euglena have a sexual cycle?
Yes No
2. Paramecium
Although you will spend more time looking at animal-like protists ("protozoa") in Bio 102, one example is
added here.
Cell Reproduction 30/11/2009
What characteristics do these cells have that are like animals? Are like plants?
3. Chlamydomonas
This small organism requires that you use a high-power objective for viewing.
If you look carefully, you may observe in each tiny cell: a cup-shaped chloroplast with a round starch-like
product in the middle, two flagella, an eyespot (similar to Euglena), and you may even discern the centrally-located
nucleus.
3. Chlamydomonas fusion
This small organism has at least two "mating types". Since you cannot tell one from the other microscopically,
Click the thumbnail below to view a video of the first stage of mating, where plus and minus types "clump"
together.
Cell Reproduction 30/11/2009
Next, click the thumbnail below to view a video clip of "pairing", where two Chlamydomonas cells come
together to mate. Watch the two cells at the top of the window.
After pairing, fusion begins. On the right, each mating stage is shown in its correct temporal sequence.
Chlamydomonas cells have a sexual cycle, but may also reproduce clonally, by undergoing mitotic division, as
shown below.
Cell Reproduction 30/11/2009
haploid diploid
How can you distinguish the haploid from the diploid phases in Chlamydomonas? In any organism?
You have observed "fertilization" in this organism. What compensating process must occur in the diploid (2n)
cells?
Mitosis Meiosis
In the figure below, identify which cells are haploid and which are diploid.
1. Oedogonium
Note the top image on the right, from a prepared slide of this alga. It shows the chain of cells that makes up
In the middle image, note the clear, round structures within some of the cells; these are the eggs.
As in Chlamydomonas, the zygote is the only diploid structure in the alga's life cycle. The Chlamydomonas
zygote goes through meiosis to form four mobile "zoospores" that swim about, eventually becoming attached to
the lake bottom and differentiating into a new filament. This organism also reproduces asexually by simple
fragmentation or by the formation and liberation of single zoospores from vegetative cells.
Cell Reproduction 30/11/2009
filament.
distinguished by their thickened cell walls, increased starch content, and darker
2. Spirogyra
Cell Reproduction 30/11/2009
This is another filamentous alga, commonly found in ponds and puddles around New Jersey. From wet mounts,
and the figure below, you can see that it is also a long filament of cells.
Note the shape of the chloroplast(s) in the figure below. The protoplast of the cell is mostly transparent; therefore,
you can see both the front and back of the single spiral-shaped chloroplast that gives this genus its name.
Fusion in this genus begins as two filaments attach. In the image below, notice the small conjugation tube that
Conjugation is complete after the cytoplasm from the adjacent cells fuse to form zygotes.
The heavily stained ovoid structures in the figure below are zygotes. Notice that the filament on the left is
empty of cytoplasm, and though cell walls remain visible, you can no longer see the spring-like chloroplasts inside.
The marine alga Fucus has adapted to life in the intertidal region: part of the day it is submerged, while other
a. Look at the images of Fucus below. Note the various specialized structures such as holdfast, air bladder,
Fucus
b. Inside the receptacles are small pit-like areas that contain the male and female reproductive
structures. Examine images of these conceptacles below. How are male and female structures similar? How do
they differ?
The male conceptacle (above) can be identified by the many small, darkly-stained, antheridia. The
antheridia contain sperm or sperm progenitors. The lighter-stained thread-like hairs are non-sexual; they help
The female conceptacle is characterized by its large, bulb-shaped oogonia with egg or egg progenitor
cells inside. As in the male conceptacle, sterile hairs protect against dessication.
b. Male and female reproductive structures are located inside the conceptacles. Examine images of the
antheridia and oogonia below. How are these male and female structures similar? How do they differ?
In these higher magnification images and diagrams, compare the small, slim antheridia (above) with the bigger,
rounder oogonium (below). Reproductive structures are stained red in the micrographs, sterile hairs appear blue.
Study the life cycle of Fucus below. Decide whether each structure is part of the haploid or diploid phase of the life
cycle.
The sexual cycles of the fungi are basically the same as you have seen before, with the zygote being the only
diploid cell in the entire cycle. However, in fungi the fusion of the cytoplasm, or plasmogamy, is not immediately
followed by the fusion of the nuclei, or karyogamy. The stage between plasmogamy and karyogamy is called
dikaryotic, or (n + n), since each cell has two haploid nuclei: one from the plus strain and one from the
minus strain. Each mitotic division therefore results in two pair of nuclei that are dikaryotic, each having
one nucleus from the plus strain and one from the minus strain.
In the diagram above, each arrowhead represents an event in the life cycle of many fungal cells.
• On an amenable medium, Rhizopus can be seen with the naked eye, appearing as a fuzzy white mat
growing all over the surface. (And down into the medium. And even over the top of the petri dish
sometimes.) This is the mycelium, or "body" of a fungus. Each strand is called a hypha
(plural, hyphae).
• Through a microscope, one may note small, black balls growing at the ends of certain hyphae. These
are the sporangia. Sporangia can be seen in the image of Rhizopus at the right (top). Note that each
the fungus.
• After the organisms in a culture initiate the sexual cycle, one may find zygotes (actually, zygospores
composed of numerous nuclei) in regions where the plus and minus join.
Rhizopus:
Rhizopus sporangium
objective. Rhizopus
In the figure below, identify which cells are haploid and which are diploid.
Cell Reproduction 30/11/2009
B. Mushrooms
Basidiospores are formed by meiosis. Remember that the product of a meiotic division is four daughter
cells. Therefore, in Coprinus four basisiospores arise from each basidium. Can you find all four basidiospores
Figure 1 above: Diagram of cross section through mushroom cap. Notice the gills radiating from the
central stalk. Figures 2 and 3: Higher magnification (as seen through 10x objective) through the basidiocarp.
Notice the basidiospores (stained red in Figure 2, above, and Figure 4, below) attached to gills at basidia.
Figure 4 above: Basidiocarp gills as seen through 40x objective. Notice the different stages of
development of basidiospores.
Haploid or Diploid
Coprinus
Cell Reproduction 30/11/2009
In the life cycle below determine if each tissue is haploid, diploid, or dikaryotic (n + n). Remember that
Make a diagram like the one below, showing how one dikaryotic cell can divide by mitosis to form two
C. A Cup Fungus
Cell Reproduction 30/11/2009
Examine the diagrams and images of Peziza (an ascomycete) that show longitudinal sections through the
fruiting structure, or "cup". In the lower image, look for the sac-like structures, or asci, holding the spores (each
ascus contains 8 ascospores, numbered in one ascus in the lower image). The eight ascospores are formed by
meiosis (to produce 4 cells) followed by a mitotic division (to double the number to 8).
Peziza
Cell Reproduction 30/11/2009
The life cycle is similar to that for basidiomycetes (like Coprinus) except the dikaryotic stage is much
shorter.
Are the spores part of the sexual or asexual cycle of a basidiomycete's life history?
Sexual or Asexual
Plant Evolution 30/11/2009
Plant Evolution
Objectives:
• 1. To look at cellular specialization in plants, with emphasis on lignified cells that function
• 3. To examine the evolution of sexual cycles in a moss, a typical fern, and a gymnosperm.
By the end of this exercise, you should be able to identify the various lignified cell types in cross sections
of stem. You should know how to tell a moss from a fern; a gametophyte from a sporophyte. You should
understand which cells are haploid and which diploid, and whether they are formed by mitosis, meiosis, or
fertilization. You should be able to apply this information to the reproductive cycle in gymnosperms such as pine.
Section 1:
The evolution of multicellular plants involved the modification of different cells to perform distinct
functions. Part of the success of land plants was based upon the evolution of a very strong molecule called lignin.
In this laboratory you will learn how to identify three lignified cells.
Reproduction: Protists and Fungi 30/11/2009
• 1.Tracheids: first to evolve, combined the functions of support and water transport.
• 2.Vessel elements: subsequently evolved from tracheids, specialized for water transport.
All three can be found in xylem, while fibers can be found in xylem, phloem, and other parts of the plant.
A. Moss
1. Observe the living green "leafy" gametophyte stage on the right. Remember that these organisms have
leaf-like, stem-like, and root-like structures. You can see more of the details by magnifying the image.
Reproduction: Protists and Fungi 30/11/2009
2. Now examine the cross section of a moss (Mnium) "stem". You can see the general structure of the "stem."
• Find:
a. the outer epidermal cells - Epidermal cells are located on the outer wall of the moss stem.
b. the "leaves" attached to the epidermis - "Leaves" are an extension of the epidermis.
c. the multicellular cortex region - The cortex is composed of all the tissue from the epidermal
d. the transport tissue - The transport tissue is located in the middle of the stem.
Reproduction: Protists and Fungi 30/11/2009
(Although some of the cell walls in the cortex are stained red, moss is a nonlignified plant.
The central portion of the "stem" is composed of much thinner cells. While the central cells are specialized
to transport water, they are not lignified so they are not called "vascular tissue." [And therefore, moss plants do
B. Ferns
Reproduction: Protists and Fungi 30/11/2009
1. Examine the pictures of live ferns. How tall are these sporophytes? What structures are specialized for
photosynthesis?
2. Study the cross section of a fern rhizome (an underground stem) of the Cinnamon fern, Osmunda
cinnamomea. In the fern, the leaves extend in clusters from an upright position of the stem. Thus, you will find a
central vascular region surrounded by a ring of vascular bundles; each smaller bundle extends to a different leaf,
• Find:
As is the case with most ferns, the xylem in this fern is composed of tracheids only. (Remember, lignified
cell walls are stained red -- along with tannins and other materials with a net negative charge.)
C. Pine (a gymnosperm)
1. Examine the picture of a live pine tree. How tall is the sporophyte stage? Are there specialized
photosynthetic structures, and other portions of the plant to support them? Do these organisms have specific
2. Examine the cross sections of a young twig on the right of a 3-year-old pine (Pinus) stem.
Reproduction: Protists and Fungi 30/11/2009
Reproduction: Protists and Fungi 30/11/2009
c. the bark
1. cork
2. cortex
3. phloem
Now click here to magnify the image even more. You should see more of the cellular detail.
Reproduction: Protists and Fungi 30/11/2009
You should notice that all of the cells with red cell walls look alike -- other than slight differences in
diameter in the early "spring" wood and the late "summer" wood found in each annual ring. Remember that in pine
wood, the only lignified cells are tracheids, cells that function both for water transport and support.
The cylindrical structures surrounded by green-staining cells are the resin canals. Under low
magnification you can see them in both the woody part of the twig as well as in the bark. This resin makes the cut
a. the tracheids
c. the thin-walled phloem cells that transport "food" throughout the tree
d. the protective cell that surrounds the outside of the stem (What are they called?)
Since it is difficult to determine the cell types using cross sections, an alternative method can be used
called "maceration." The wood is treated with chemicals to make the cells come apart. Examine the macerated pine
wood on the right to see what the tracheids look like from the side. Note the bordered pits in the cell walls. Click on
D. Angiosperms
• Find:
c. the bark
1. phloem
2. cortex
3. cork
Notice that although the water conducting cells in pine wood were fairly uniform (Pinus stem), those in
angiosperm wood are composed of at least two cell types. Click here.
Reproduction: Protists and Fungi 30/11/2009
Reproduction: Protists and Fungi 30/11/2009
The lignified cells with the largest diameters are the vessel elements. The lignified cells with smaller
diameters are either tracheids or fibers; the difference can only be determined from a side view (see diagram of
In the outer portion of the twig, the outermost layer is cork and the inner bark is phloem. But note that
there are lignified cells in the phloem region also! Click here.
Under higher magnification you will be able to see that the red-stained cell walls are very thick, and that
the hollow cavity is very narrow. Therefore, if you identify anything with red-stained cell walls as xylem, you may
It is easy to tell vessel elements in cross sections because of their size, but more difficult to distinguish a
tracheid from a fiber. Therefore, examine the prepared slide of macerated basswood . You can click on the image
to magnify it.
Reproduction: Protists and Fungi 30/11/2009
Reproduction: Protists and Fungi 30/11/2009
• Identify:
a. a tracheid
b. a fiber
c. a vessel element
2. the pith
3. the cortex
1. vessel elements
2. fibers
Reproduction: Protists and Fungi 30/11/2009
There are lignified cells in the portion of each vascular bundle closest to the outside (cortex) and other
lignified cells in each bundle closest to the pith. The large diameters of the lignified cells on the inside are indicative
of vessel elements, and are therefore in the xylem. However, the cells on the outside have red-stained cell walls
that are very thick, and the hollow cavity is very narrow. Once again, you are seeing fibers. And, once again, since
this is the phloem on the outside of each bundle, these are phloem fibers.
Section 2
The evolution of plants also involved alterations in the sexual cycle. All plants have the sexual cycle that
alternates a diploid sporophyte generation and a haploid gametophyte generation. The sporophyte forms spores
In moss, the gametophyte is "dominant" (or predominant; you are more likely to encounter this phase of
the sexual cycle). In ferns and all other vascular plants, the sporophyte is predominant. In ferns (and other
"seedless vascular plants") the gametophyte is a free-living, autotrophic organism. While the fern sporophyte is
well adapted to land, the fern gametophyte is not. Therefore, in the subsequent evolution of land plants, this
haploid portion of the sexual cycle was modified in several ways that allow these plants to survive increasingly dry
Remember that in the last lab you learned two types of sexual cycles.
• In one, fertilization is immediately followed by meiosis, the only diploid cell being the zygote (top).
• In the other, meiosis is immediately followed by fertilization, the only haploid cells being the
• A third cycle (center) occurs in all plants and some multicellular protists. In this cycle, haploid
spores are formed by meiosis. These spores divide by mitosis to produce a multicellular haploid phase
that produces gametes by mitosis. The zygote formed by the fusion of two gametes divides by mitosis
In the alternation of haploid and diploid generations the diploid phase produces spores and is therefore
called the sporophyte (“spore-plant”). The haploid phase produces gametes and is therefore called the
NOTE: Both the gametophyte and the sporophyte stage may be duplicated by asexual reproduction - the
This basic alternation of generations has two variations. In the bryophytes such as moss, the
gametophyte generation is predominant ("dominant") and the sporophyte grows out of the gametophyte (right
bottom). In all vascular plants the sporophyte is predominant (left bottom); the gametophyte may be free-living,
ALWAYS REMEMBER:
Mitosis: one diploid cell dividing to form two diploid cells - or - one haploid cell dividing to form two
haploid cells
Fertilization: two haploid cells fusing to form one diploid cell (the zygote)
A. Moss
1. Study the image of a moss antheridium. What type of cells are formed in these structures? Click on the
2. Now study the image of a moss archegonium. If it is a good section, you will see a large cell (egg)
within a vase-like structure. The sperm must swim through liquid water to the opening at the top of the vase, then
swim down the channel before the egg is fertilized, forming the zygote. Click on the image or the word "magnify"
3. Examine the whole moss plant in the sporophyte stage. Do you think this brown structure can carry on
a. sporophyte or..
b. gametophyte
Reproduction: Protists and Fungi 30/11/2009
• Label:
a. antheridium
b. sperm
c. archegonium
d. egg
e. zygote
f. spore
g. protonema
NOTE:If you should discover living sporophytes outdoors, you can pass your hand across the top. The
results will be a cloud of fine particles. These are the spores formed by meiosis that will germinate to develop into
B. Ferns
Reproduction: Protists and Fungi 30/11/2009
1. Examine the live fern leaf ("frond") that has brown or black specks ("sori") on the underside. The small
brown or black cells are spores that, under the correct conditions, will germinate to form the gametophyte
generation. Some ferns, such as the cinnamon fern (previous section), have all of the sporangia on a separate
"fertile fond." The sporophyte cells are diploid; the spores (and gametophyte cells) are haploid. Were the spores
2. Examine the images of the fern antheridium and archegonium. Click on the images to get a closer look
at the cells.
Reproduction: Protists and Fungi 30/11/2009
Reproduction: Protists and Fungi 30/11/2009
Reproduction: Protists and Fungi 30/11/2009
a) sporophyte or..
b)gametophyte
• Label:
a. antheridium
b. sperm
c. archegonium
d. egg
e. zygote
f. spore
C. Pine
In both of the plants you have observed so far, the spores formed by meiosis all look the same; the plants were
homosporous. A variation on this theme is where some spores are larger than others; these plants are
Each scale in the smaller male "staminate" cones contains numerous diploid microsporocytes or
"microspore" mother cells that go through meiosis, each forming four haploid microspores. Each microspore
undergoes several mitotic divisions to form a microgametophyte, or pollen grain. Some of the nuclei, the sperm
The scale in the larger female ("ovulate") cone has two ovules, each containing a single megasporocyte
(megaspore) mother cell which goes through meiosis to form four haploid megaspores. In this case, three
megaspores degenerate while the fourth continues to divide mitotically, forming a multicellular
megagametophyte. This megagametophyte has two archegonia, each containing one egg.
What kinds of cells are found in these cones? Are the cells released from these cones haploid or diploid?
You can see pollen being released from the male pine cones in the third image on the right.
Reproduction: Protists and Fungi 30/11/2009
The forth image on the right is a magnified picture of actual pollen grains.
Reproduction: Protists and Fungi 30/11/2009
• 2. Now observe the image of the larger female (ovulate) pine cone, the type you usually see in pine
cone wreaths.
Reproduction: Protists and Fungi 30/11/2009
What kinds of cells were formed WITHIN each of these leaf-like scales? The sixth image on the right
depicts a pine seed found within the scales of the female pine cone.
How do the gametes produced by the male cone get to the egg in the female cone? And THEN what
happens?
• 3. Examine a seed which is cut open, the seventh image on the right.
Reproduction: Protists and Fungi 30/11/2009
Look for the embryo with its numerous cotyledons. Look for the white storage tissues around the embryo.
What specialized structures help disperse these seeds away from the parent plant? (And why is this important?)
a) sporophyte or..
b) gametophyte
• Label:
a. megaspores
b. megagametophyte
c. archegonium
d. microspore
e. microgametophyte (= pollen)
f. pollen tube
g. embryo
h. seed coat
i. seedling
Pine is only one example of a gymnosperm. What other gymnosperms still live on earth? How are they
III Review:
This is a complicated lab, with lots of parts, so it is essential that you get an overview before try to sort
• 1. Think about all of the figures that illustrate tracheids. What are the functions for tracheids? How
can you identify a tracheid in a cross section? What plants did you observe today that had tracheids?
• 2. Think about all of the figures that illustrate vessel elements. What is the main function of a
vessel element? How can you identify a vessel element in a cross section? What plants did you
observe today that had vessel elements? What do they have in common?
• 3. Think about all of the figures that illustrate fibers. What is the main function of a fiber? How can
you identify a fiber in a cross section? In what tissues did you find fibers? What plants did you
• 4. Compare the sexual cycles for moss and ferns. What do they have in common? How do they differ?
• 5. Compare the sexual cycles for ferns and pine. What do they have in common? How do they differ?
• 6. The "basic life cycle" illustrated in this lab is for a homosporous plant, such as most ferns. Change
this life cycle to represent the situation for a heterosporous plant, such as pine.
Reproduction: Protists and Fungi 30/11/2009
Angiosperm Reproduction 30/11/2009
Angiosperm Reproduction
Objectives:
• To study the development of the different parts of the flower, and how these regions
• To observe some of the variation in different parts of sample fruits, and relate these
After completing the laboratory exercise you should know the parts of a flower and the functions of each.
You should be able to recall which part of the flower develops into each portion of a fruit. Again, you should be able
to recognize which cells are haploid and which diploid, and whether they are formed by mitosis or meiosis. Finally,
you should appreciate how plants have evolved different structures for pollen transfer and for fruit dispersal.
Introduction:
This lab is a continuation of the previous lab in which you studied some of the basic aspects of plant
evolution. Click here to briefly review the evolution of land plants. Next, review the generalized life cycle at the
Finally, review the evolution of the gametophyte generation, starting as a free-living, multicellular,
photosynthetic organism in ferns. In gymnosperms like pine, the microgametophyte was reduced to a small, wind-
blown structure called "pollen". The megagametophyte was maintained within the sporophyte cone, and
surrounded by the sporophyte layer called the integument (later to become the seed coat). The megagametophyte
was still large enough to provide the nutrients for the young embryo in the mature seed. This trend of reducing the
gametophyte generation is continued in angiosperms. These and other trends in plant evolution are diagrammed
In this laboratory, you will observe several of the structures associated with the reproductive portion of
the plant life cycle. You will start by observing the development of the megagametophyte ("embryo sac") as well as
that of the microgametophyte ("pollen"). You will then study the subsequent development of the seed and fruit.
You will also examine examples of the different types of fruits to see how they have evolved different
I. The Flower
You have now come to a major evolutionary advancement of the plant kingdom. Review the main parts of
In the diagram, parts of the male reproductive structures, or stamen, are labeled in green. There are six
The female reproductive structures are labeled in blue. The stigma, style and ovary make up the single
pistil in this flower. Note that the ovary is not visible in the diagram, but is located in the center of the flower, at
The diagram below illustrates the components associated with sexual reproduction.
On the left, label the parts of the stamen, the male reproductive structures.
On the right, label the external parts of the pistil (sometimes also called the "carpel"), the female
reproductive structure.
1.Anther
2. Filament
3. Stigma
4. Style
5. Ovary
each:
• Petal: To attract pollinators with colors and/or odors; to provide a landing area for insect pollinators.
We can speculate that one of the functions of sepals is to hide developing flowers from pollinators. This
prevents colored petals from attracting insect visitors before pollen is ready for dispersal, and may be one of the
selective advantages for having inconspicuously colored sepals--they are often green! They may also protect fragile
flower parts from damage, and so they are sometimes thicker, or fleshier, than petals. Are there other ways to
The figure below depicts the internal features of the pistil after pollination. Label the parts and identify
1. Pollen grains
2. Stigma
3. Style
4. Pollen tube
5. Ovary
6. Ovule
7.
If the petals and sepals are removed from a gladeolus flower, the remaining
parts are the stamens and the pistil (sometimes called the carpel.)
When a longitudinal cut is made down the ovary, the line of ovules inside
becomes visible.
The following diagram shows the gladeolus flower dissected in this way.
A. The Anther
Plant Evolution 30/11/2009
Study the diagram below, which illustrates a cross section through flower sexual structures. Note the style
in the center of the circle of anthers. Each anther consists of two pollen sacs, one on each side of a vascular
bundle.
1. Stigma
2. Style
3. Ovary
4. Ovules
5. Anther
6. Filament
1. Anther (pollen sacs are within the anthers, two pairs per anther.)
2. Style
Compare the diagram above with the prepared slide of the Lilium anther, to the right. This is a large
structure-- this image was captured with the 6x scanning lens! Scroll to find the style, anthers (and their pollen
Two features of the pollen are worth noting: First, each pollen grain has two nuclei. (You will have to look
to find a pollen grain where the histological section was made in such a manner that both nuclei were in the same
slice.) Second, the cell wall has a definite structure. (Look for grains where the section was right across the upper
Examine a prepared anther using higher power by clicking on the image below to magnify.
If the field of view in the thumbnail image below is 1.2mm, how large is one pollen grain? Find a cluster of
grains that line up across the diameter of the field. Count the number of grains and divide this into the known field
of view.
Plant Evolution 30/11/2009
1.
2.
3.
You may review the procedures for estimating cell size using a known field of view in Laboratory 1.
Do you estimate that the lily pollen is 0.003 mm, 0.05 mm, or 0.1 mm?
Microgametophyte Development
The development of the microgametophyte (male gametophyte) in flowering plants involves three stages:
• A diploid microsporocyte, or microspore mother cell, divides to give rise to four haploid
microspores.
Plant Evolution 30/11/2009
• Each microspore divides by mitosis to form two haploid nuclei (the tube nucleus and the generative
• After landing on the stigma, the pollen germinates forming a pollen tube; the generative nucleus
B. The Ovary
Plant Evolution 30/11/2009
The image below is an illustration of a cross section through Lilium ovary, as might be seen using a
scanning lens.
In the Lilium ovary, the ovules are attached in pairs to a central stalk. In prepared slides, you may not
In the image to the right, note the small vascular strands in the central region, the ovules, and the
2, 3, or 6?
The development of the megagametophyte ("embryo sac") in most angiosperms involves three stages.
A diploid megasporocyte (megaspore mother cell) undergoes meiosis, giving rise to four haploid
The remaining megaspore nucleus undergoes three mitotic divisions to form eight haploid nuclei.
While the above is "typical", the number of antipodals can vary from none to many, depending upon the
species. The smallest megagametophyte only has an egg cell plus one polar nucleus.
A. Development of a Seed
Capsella: a dicot
Plant Evolution 30/11/2009
Examine several slides of the cosmopolitan weed Capsella bursa-pastoris (Shepherd's purse). This plant
has numerous heart-shaped fruits ("purses") on a long stem. Each of these fruits contains dozens of minute seeds.
(See the illustration below.) The white flowers and developing fruits are at the very top of the plant, and fruit at
Review some of the basic parts of the developing seed using the illustrations below.
Plant Evolution 30/11/2009
Note that in this plant the endosperm is absorbed by the developing cotyledons, so that the stored food in
the mature seed is in the cotyledons. (The same pattern is also true for the legumes, including the pea and bean
The sections from the previous illustrations, and in the illustration below, are exactly parallel to the
embryonic axis. As you examine the prepared slides, however, you will note that many of the seeds have been cut
at other angles. Use the following illustration to help understand these sections.
Plant Evolution 30/11/2009
The diagram above shows four of the infinite planes where the seed could be sectioned. The angle of the
cut is shown in blue. Identify what the resulting section would look like if the section were made at the blue line.
• B. Fruit Development
Fruit development is a complex process, varying considerably among different plant groups. Three
examples of the flower and then the fruit are found in the figures to the right.
For example, in peas the flower is "irregular" (that is, it has bilateral rather than radial symmetry), with
sepals and petals modified into several different shapes. The ovules are attached down a single row in the ovary so
that the seeds develop in the same way. When you split open a pea pod, you can get a good impression of how the
Complete your collection of life histories by adding two angiosperm sexual cycles.
The figure on the right is the sexual cycle of a dicot, the bush bean.
Plant Evolution 30/11/2009
While completing the corn life cycle, note that while the materials in the endosperm have been
transported to the cotyledons during bean seed development (as in Capsella), the endosperm still exists in the
C. Fruit Diversity
Plant Evolution 30/11/2009
• Dispersal: Angiosperms have evolved a number of different methods for protection and dispersal of
the next generation. These dispersal mechanisms range from structural modifications such as barbs
that stick in fur, or buoyant structures that carry fruits and/or seeds through water; to sweet fruits
that are eaten by animals that disperse seeds (such as the peach, on the right).
• Domestication: A variety of plants have been cultivated, often because of the fruits. The original
evolution of the fruit was for dispersal. For example, note the three layers of a peach on the image
below.
The Peach:
The outer layer ("skin") protects the next layer from drying out. The main central layer attracts animals,
who consume the whole fruit. The inner layer ("pit") protects the seed during the process.
The pit is later deposited, along with some "fertilizer", after in has passed through the animal's gut.
To continue, click on the area of the image that was the ovule when this fruit was still a flower.
Plant Evolution 30/11/2009
3. Significance of grains
The kernel is technically a fruit, not a seed, since the seed coat and fruit wall are fused.
Examine the prepared slide of a corn kernel above. (The scientific name for corn is Zea mays).
Biology Lab Practical Lab 9
Objectives:
• 2. To investigate the structures associated with transport of water and of organic nutrients.
Introduction:
The transport systems in plants are quite different than the circulatory system found in vertebrates.
Vascular plants have two transport systems, one to move water (and the minerals dissolved in it) from the roots to
the leaves and the other to move organic compounds (mainly sucrose) from the "source" to the "sink". The cells
responsible for long-range water transport have already been studied in Lab 7: the tracheids and vessel elements
found in xylem. The cells responsible for long-range transport of organic compounds are found in the phloem.
The hierarchial nomenclature used in plant anatomy is often confusing, and is therefore outlined to the
right. Click here to get table. Note that both xylem and phloem tissues may also contain cell types that do not
1. confirm the path of water movement between the uptake by root hairs and the loss
through stomata
The purpose of this experiment is to examine the movement and pathway of water up an Impatiens
stem. In the first part of the experiment, congo red dye will be added to the water to trace the pathway of
water up the stem. Cross-sections of the stem demonstrate where in the stem the water is moving and oblique-
sections allow identification of the actual cells involved in water transport. The second part of the experiment
identifies which parts of the stem, and more specifically, which cells, are lignified. Are the cells involved in
Part I :
Step 1: Impatients have translucent stems (due to the presence of large, thin-walled cells). Therefore, the
vascular strands running longitudinally in the stem can be observed directly. Notice the strands in the picture to
the right. Click on the picture to label the strands with arrows.
Step 2: Cut the root system from the plant and immediately place the cut surface of the stem in water.
Cut off another centimeter of stem under water to ensure there are no air bubbles in the transport tissue that could
disrupt water transfer. Click here to see how that was done.
Angiosperm Reproduction 30/11/2009
Step 3: Quickly transfer the cut stem to a test tube containing a solution of Congo red and prepare your
controls while you wait for water transport to occur up through the stem. Click here to observe the congo red set-
up.
Angiosperm Reproduction 30/11/2009
Angiosperm Reproduction 30/11/2009
Step 4: Two controls are needed for this experiment. Since the purpose of the experiment is to trace
the movement of congo red dye to follow the pathway of water transport, it is first necessary to see if any of the
cell walls of the plant are already stained red. To do this mount a thinly cut section of stem from the piece cut off
in step 2 on a microscope slide and view it under a dissecting microscope. This is control
1. The second control is prepared in the same way, however, this control is to check which parts of
the stem Congo red will stain (Congo red stains cellulose). Add a drop of Congo red to a second
• a.Preparation of sections
Angiosperm Reproduction 30/11/2009
Step 5: After the dye has moved to the stem apex, cut cross-sections of the stem at various points
along the stem. Examine the location of the congo red. Also make longitudinal sections at the same points to
identify the cell type. Click on the following to observe each part of step 5.
Part II:
The purpose of the second part of the experiment is to determine whether or not the cells involved in
water transport are lignified. Click on each step to observe the procedure.
• Step1:Cut additional cross and longitudinal sections to stain lignified cell walls using
phloroglucinol.
Angiosperm Reproduction 30/11/2009
• Step5:Place a cover slip over the sections and examine it under the dissecting microscope.
Angiosperm Reproduction 30/11/2009
Angiosperm Reproduction 30/11/2009
water evaporating through the stomata produces a tension, or negative pressure, that pulls the water
column up the plant. This column is maintained by the hydrogen bonding between water molecules (cohesion)
and the hydrogen bonding between water molecules and the molecules that make up the cell walls (adhesion).
If the above mechanism is correct, then a twig that has been removed from the rest of the plant and
attached to an artificial water column (as in a pipette) could pull water up the pipette. The rate of movement can
Begin Experiment:
Angiosperm Reproduction 30/11/2009
1. Place a transport apparatus (1 mL pipette with a piece of clear tubing on the larger end) under water
using the large dish pan. Manipulate the apparatus until it is completely filled with water - NO air bubbles.
To do this, click on the yellow tubing to place it on the pipette. Next, click on the pipette to
2. Place the cut end of the juniper twig under water and cut off another centimeter to insure that there
are no bubbles in the transport tissue that would disrupt water transport. Insert the freshly cut end into the above
tubing while under water, making certain that NO air bubbles are trapped within the assembly. If the twig does not
fit tightly in the clear tubing, wrap several strips of Parafilm around the stem before inserting it into the tubing.
Click on the juniper twig to place it into the dish pan. Click on the knife to cut off an additional
cm under water. Next click on the twig again to insert it into the tubing. For our purposes, assume it
fits snugly.
3. Add about 20 mL of red colored dye (Congo red) to the bottom of a small beaker. With your finger over
the tip of the pipette, transfer the assembly so that the tip is now into the dye. Remember: NO air bubbles!
Click on the test tube to add the Congo red. Next click on the pipette/twig assembly to place it
4. Transfer the whole thing to the ring stand. Clamp it into place and turn on the light at the top of the
assembly.
5. This same set-up is repeated with a juniper stem without leaves to use as a control.
6. At periodic intervals, look for a movement of dye up the pipette. Click here to start the experiment.
Angiosperm Reproduction 30/11/2009
NOTE: The movement of the dye observed takes approximately half an hour in real time.
Click here to see the set-up and results of this experiment from a laboratory class. Click on either beaker
A. The Root
1. Whole Roots
Before examining the root ultrastructure, lets take a look at the parts of the whole root. We're going to
Next click on the roots to examine a single root shoot. Note that the Cyperus has a large primary root
(thick black arrow). Thinner secondary roots (thin black arrow) emerge from the older primary root.
Angiosperm Reproduction 30/11/2009
Note how the secondary roots originate at a definite position behind the root apex and become
progressively longer (older) as you go up the primary root. This form of continuous development is characteristic of
Now click on the Begonia roots. This plant had been clipped and the stem was placed in water. The
stem grew new root shoots. Notice the numerous root hairs. The root hairs increase the surface area of the
root.
Angiosperm Reproduction 30/11/2009
Below to your left, view the cross section of the root as if you were using a microscope. Increase
magnification by using the + sign and decrease magnification using the - sign. You can also move the stage of the
microscope by clicking on the button on the far right and moving the hand. Once you have examined the section,
label the parts of the root by clicking on the image to your right. Identify the epidermis and the xylem at low
magnification and identify the phloem cells, cortex, and endodermis at high magnification.
Indentify:
Epidermis at low magnification – The epidermis is a one-cell thick outer covering of the root.
Identify:
Angiosperm Reproduction 30/11/2009
• phloem cells – The phloem cells are the cluster of green-stained cells in the arms of the X-shaped
xylem
• Vessel Elements - The vessel elements are the large conducting cells in the xylem in the middle of
• cortex - The cortex is the intermediate portion of the bark, between the epidermis and the vascular
tissue.
• endodermis at high magnification - The endodermis is a single layer of cells that encircles the
vascular cylinder.
Angiosperm Reproduction 30/11/2009
Just as in the root exercise, the image to your left can be manipulated as if viewed through a microscope.
Click on the image to your right to identify the epidermis, cortex, and vascular bundles. You may then click on
"high magnification" to label the parts of the vascular region. Identify the phloem fibers, sieve tube members,
Identify:
Epidermis - The epidermis is a one-cell thick outer covering of the stem. It has a thick waxy cuticle and
stomata.
cortex - The cortex is external from the vascular bundles and internal from the epidermis.
vascular bundles –
Angiosperm Reproduction 30/11/2009
Identify:
phloem fibers - The phloem fibers are clustered toward the outside of the vascular bundle, forming a cap
sieve tube members - The seive tube members are located in the phloem. They are on the inner side of
vessel elements - The vessel elements are the larger, more efficient conducting cells in the xylem.
tracheids. – The tracheids are the smaller conducting cells located in the xylem.
C. The Leaf
Angiosperm Reproduction 30/11/2009
1. Whole Leafs
Before we examine the ultrastructure of the leaf, lets take a look at a whole living leaf. Notice the two
leafs on your right. The top leaf is from the plant zebrine
Notice the major and minor veins (vascular bundles) running throughout the leafs. The major
vein(s) are labeled with a large arrow(s) and the minor vein(s) are labeled with small arrow(s).
It is also possible to visualize the stomata on the leaf's surface. To do so, each leaf was painted with
clear nail polish on both sides. The film of nail polish was peeled off and mounted on a slide to examine under the
microscope. If all goes well, an impression of the leaf cells on the surface will be left. Click on the zebrina leaf to
examine the leaf's surface. Notice which surface the stomata are located and their distribution. Next click on the
coleus leaf.
Angiosperm Reproduction 30/11/2009
Angiosperm Reproduction 30/11/2009
Angiosperm Reproduction 30/11/2009
Again, the image to your left can be manipulated as if viewed through a microscope. Click on the image to
your right to identify the upper epidermis, lower epidermis, cuticle, guard cells, stoma, palisade
Identify:
upper epidermis - The upper epidermis is a one-cell thick outer covering of the top of the leaf.
Angiosperm Reproduction 30/11/2009
lower epidermis - The lower epidermis is a one-cell thick outer covering of the bottom of the leaf.
Cuticle - The cuticle is the waxy layer secreted by the upper epidermal cells to reduce water loss. In this
guard cells - Guard cells are specialized lower epidermal cells that are responsible for opening and
Stoma - The stoma is a minute air space opening at the base of the leaf.
palisade mesophyll (with chloroplasts) - The palisade mesophyll is the layer of closely packed
spongy mesophyll - The spongy mesophyll is the layer of loosely and irregularly arranged cells near the
lower epidermis.
Angiosperm Reproduction 30/11/2009
Chromosome Structure and Meiosis 30/11/2009
Objectives:
Introduction:
Biology Lab Practical Lab 9
Meiosis is the second important kind of nuclear division. It resembles mitosis in many ways
but the consequences of meiotic divisions are very different from those of mitotic divisions. While
mitotic division may occur in almost any living cell of an organism, meiosis occurs only in special cells.
In animals, meiosis is restricted to cells that form gametes (eggs and sperm). Each species has a
characteristic number of
complement of 46 chromosomes is
referred to as the diploid number (referring to the fact that each kind of chromosome is represented
twice). In higher organisms when an egg is fertilized the egg and sperm fuse to form a single cell
called a zygote which develops into a new organism. If the egg and sperm were both diploid (46
chromosomes each in the case of humans) then the resulting zygote would be tetraploid. This would
be an intolerable situation, so a mechanism has evolved to insure that each gamete (egg or sperm)
contains only one representative of each homologous pair (or half the diploid number). This is referred
The mechanism that makes this possible is meiosis. Meiosis consists of two divisions, Meiosis
I and Meiosis II, and can potentially result in the production of four cells. However the DNA is only
synthesized once (prior to Meiosis I). The subdivisions of meiosis are named like the subdivisions of
mitosis (prophase, metaphase, anaphase, telophase) but as we shall see the events are somewhat
different.
Part I:
1. Study the diagrammatic summary of cell division in Meiosis I and Meiosis II in your
Part II:
You are now ready to find the actual stages of meiosis, using the lily anther. The lily flower
has six anthers surrounding one carpel. Each anther has two pair of microsporangia ("pollen sacs"). It
reality. Therefore, you will have to look at several different cells in each microsporangium to see
exactly what is going on. Luckily, in the early stages of meiosis, all the cells in the sac are in the
same stage.
Meiosis in the anther starts with the diploid microsporocyte. Each nucleus has a diploid
number of duplicated chromosomes. These cells are still attached in the microsporangium.
Biology Lab Practical Lab 9
We will now begin to identify the different stages of meiosis. The cross-section of the lily
1. Early prophase I - In early prophase, the chromosomes are long and slender, and the bright red
nucleoli are still present. The nuclear region will be clear and the nuclear envelope is still present.
Biology Lab Practical Lab 9
2. Late prophase I - In late prophase I, the chromosomes are short, thick, and condensed. The
3. Metaphase I - During metaphase I the pairs of chromosomes are distinct and line up near the
4. Anaphase I - During anaphase I the chromosome pairs are separated and the two groups of
5. Telophase I - By telophase the two groups of chromosomes have completely separated and are
6. Interkinesis - The nuclear envelope begins to reform. The cell plate becomes visible between the
Biology Lab Practical Lab 9
In Meiosis II, all the cells in a microsporangium are not in the same stage of division.
Therefore, images of single cells have been taken for identification of these stages.
Biology Lab Practical Lab 9
Look for:
1. Prophase II - In prophase II, the chromosomes are condensed and two new nuclear envelopes
are present.
2. Metaphase II - During metaphase II the chromosomes are distinct and line up near the center of
the cell.
Biology Lab Practical Lab 9
3. Anaphase II - During anaphase II the chromatid are separated and the migrate towards opposite
4. Telophase II - By telophase the two groups of chromosomes have completely separated and are
5. Cytokinesis II - In cytokinesis, the nuclear envelope begins to reform. The cell plate becomes
visible between the dividing cells. Four cells are now visible.
Biology Lab Practical Lab 9
Part III:
You finally graduated top of your class and have taken a position as a cytogeneticist. Your
job is to construct the karyotypes of your patients in order to look for possible chromosomal
abnormalities. Each karyotype has been started for you. The first 12 chromosomes have been
matched. Your job is to match the remaining 11 chromosomes, determine whether or not your patient
has a chromosomal abnormality and if so, which one, and to diagnose the patient with the expected
symptoms of their karyotype. You better get started, your patient list is growing.....
Autosomal trisomy that produces physical malformations, and mental and developmental
retardation, so severe that most afflicted infants die within a few weeks after birth.
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9
Case 4: This is a male with the XYY karyotype. Trisomy of the sex chromosomes producing males with
less severe abnormalities, though they often have poorly developed genetalia and subnormal
intelligence. This genotype is significantly higher in individuals found in penal institutions compared to
Biology Lab Practical Lab 9
the general public and has been suggested to predispose these men to aggressive behaviour
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9
Case 5: This is a male with Trisomy 22. Autosomal trisomy in which the fetus does not survive.
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9
Case 6: This is a male with Downs Syndrome. Autosomal trisomy associated with physical features
including broad head, rounded face, perceptible epicanthic folds of the eyes, a flattened bridge of the
nose, protruding tongue, small irregular teeth, and short stature. Mental retardations is also
Biology Lab Practical Lab 9
Case 7: This is a male with Edwards Syndrome. Autosomal trisomy that produces physical
malformations, and mental and developmental retardation, so severe that most afflicted infants die
Case 8: This is a female with Patau Syndrome. Autosomal trisomy that produces physical
malformations, and mental and developmental retardation, so severe that most afflicted infants die
Now that you have correctly determined each of the above karyotypes, it is essential to
understand how they occur. The abnormalities viewed above are mostly cases of trisomies. Trisomy
chromatid to separate to opposite poles during nuclear division. When nondisjunction occurs, two
Two homologous pairs of chromosomes are present within the cell depicted below.
The large chromosomes are homologous and the small chromosomes are homologous. The
red chromosomes represent maternal genes and blue chromosomes represent paternal
genes.
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9
Depending on which gamete meets which gamete, three scenarios are possible. The gamete
with the extra chromosome could meet a normal chromosome. In this case the patient would
have a trisomy in their karyotype, as seen in the previous case studies (i.e. Down's syndrome). The
gamete missing a chromosome could meet a normal gamete. The result of this match would be
a patient with a monosomy (i.e. Turners syndrome). A normal gamete may also meet a normal
gamete in which the patient would be a normal male or female. The following illustrate these
scenarios.
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9
Biology Lab Practical Lab 9