Professional Documents
Culture Documents
Effect of Pollutants On Chlorophyll Contant in Aurangabad
Effect of Pollutants On Chlorophyll Contant in Aurangabad
Effect of Pollutants On Chlorophyll Contant in Aurangabad
1
Materials and Methods
LP P LP P P LP
Photo: Leaf showing the variation in leaf area & color due to pollution.
b) Determination of air born particulate:
The polluted leaf samples were weighed it was then washed and dried
with hot air drier the decrease in weight indicated air borne particulate
matter.
c) Determination of chlorophyll contents:
Fresh leaves were crushed in 20 ml 80% acetone and the extract was
filtered through muslin cloth. It was 100 ml by 80% acetone. The
2
absorbance of the filtrate was determined at 663 nm and 645 nm and
chlorophyll content was calculated following Harborn (1963).
Air quality monitoring was done in polluted experimental site.
3
Fig1.: Comparison of chlorophyll content between polluted & very less
polluted plants.
45
40
35
30
25 Polluted
20 Very less polluted
15
10
5
0
Ailanthus Ficus Hibiscus
excelas religiosa rosa
cinensis
4
n
9 15 11.56 29 40 33.31 91 194 130.94 318 645 443.69
Table 3 shows the concentration of pollutants of two months at experimental
site.
Reference:
Harborn, (1963). : “Phytochemical meathods”, Academic press, New York.
Joshi O.P., Wagela D.K. and Pawar K. (1997).: “Urban air pollution effect
5
Lorenc-Plucinska, G., (1982b). “Effect of sulphur dioxide on CO2
exchange in SO2 tollerant and SO2 succeptible Scots pine seedlings”.
Photosyntetica, 16 (1), 140–144.
Malhotra S.S.(1977). : “ Effect of aqueous SO2 on chlorophyll destruction
Planta, 135:25-32.
6
45
40
35
30
25 Polluted
20 Very less polluted
15
10
5
0
Ailanthus Ficus Hibiscus
excelas religiosa rosa
cinensis
7
Plant Total Chlorophyll contents(mg/g)
Polluted Less polluted