Alleleustrious pmWasabi-Peroxisomes

Catalog Number: Introduction All ustrious pmWasabi-Peroxisome is a mammalian expression vector that expresses mWasabi fused to an N-terminal Peroxisome Targeting Signal 1 (PTS, with sequence Ser-Lys-Leu). This fusion protein is localized to peroxisomes. All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. Peroxisomes are omnipresent organelles that contain enzymes for certain oxidative reactions, such as the beta-oxidation of very-long-chain fatty acids. Peroxisomes are found in virtually all eukaryotes but not in prokaryotes. Peroxisomes have a single lipid bilayer membrane that separates their contents from the cytosol and contain membrane proteins critical for importing proteins into the organelles and aiding in proliferation. Similar to lysosomes, peroxisomes are part of the secretary pathway of a cell, but they are much more dynamic and can replicate by enlarging and then dividing.
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ABP-FP-WPER

Source Engineered variant of mTFP1, originally derived from Clavularia sp. coral. Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter).........1-589 Peroxisome targeting signal.1393-1404 Linker...1387-1392 Wasabi.....679-1386 SV40 PolyA Signal.....................1555-1605 bla Promoter...............................2144-2248 Ampicillin Resistance Gene......2233-3093 pUC Origin..................................3242-3884 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA AMPR pUC Ori PCMV Peroxisome Targeting Signal Linker

pmWasabiPeroxisomes 3966bp

Wasabi

SV40 Poly A

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Saleem RA, Smith JJ, Aitchison JD (2006). "Proteomics of the peroxisome". Biochim. Biophys. Acta 1763 (12): 154151. SG Gould, GA Keller and S Subramani (1987). "Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase". The Journal of Cell Biology, Vol 105, 2923-2931.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Peroxisomes
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG GATCCACCGG CCCGACATGA GAGGGCGAGG GAGGGAGCCC AGGGCCTTCA GGCTACTCTT GACATCTCCA CCCCCCAACG ATGTACGTGC GGCGGCCACC CCCGACTATC AAGGTGACCG TACAAGTCCG TTTGTAGAGG AAAATGAATG AGCAATAGCA TTGTCCAAAC TGTGGTGGTT CGCTTTCTTC GGGGCTCCCT TTAGGGTGAT GTTGGAGTCC TATCTCGGTC AAATGAGCTG TTAGGTGGCA CATTCAAATA AAAAGGAAGA TTTTGCCTTC CAGTTGGGTG AGTTTTCGCC GCGGTATTAT CAGAATGACT GTAAGAGAAT CTGACAACGA GTAACTCGCC GACACCACGA CTTACTCTAG CCACTTCTGC GAGCGTGGGT GTAGTTATCT GAGATAGGTG CTTTAGATTG GATAATCTCA GTAGAAAAGA CAAACAAAAA CTTTTTCCGA TAGCCGTAGT CTAATCCTGT TCAAGACGAT CAGCCCAGCT GAAAGCGCCA GGAACAGGAG GTCGGGTTTC AGCCTATGGA TTTGCTCACA ATGCAT TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT TCGCCACCAT AGATCAAGCT GCGAGGGCAA CCCTGCCCTT CCAAGTACCC GGGAGCGCAC TGGAGGAGGA GCCCCGTGAT GCGACGGCGT ACCGCGTTGA ACTTTGTGGA TTTACGAGAT GATCCAAGCT TTTTACTTGC CAATTGTTGT TCACAAATTT TCATCAATGT ACGCGCAGCG CCTTCCTTTC TTAGGGTTCC GGTTCACGTA ACGTTCTTTA TATTCTTTTG ATTTAACAAA CTTTTCGGGG TGTATCCGCT GTATGAGTAT CTGTTTTTGC CACGAGTGGG CCGAAGAACG CCCGTATTGA TGGTTGAGTA TATGCAGTGC TCGGAGGACC TTGATCGTTG TGCCTGTAGC CTTCCCGGCA GCTCGGCCCT CTCGCGGTAT ACACGACGGG CCTCACTGAT ATTTAAAACT TGACCAAAAT TCAAAGGATC AACCACCGCT AGGTAACTGG TAGGCCACCA TACCAGTGGC AGTTACCGGA TGGAGCGAAC CGCTTCCCGA AGCGCACGAG GCCACCTCTG AAAACGCCAG TGTTCTTTCC TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGA GGTGAGCAAG GAAGATGGAG GCCCTACGAC CTCCTACGAC CGACGACATC CATGACCTTC CTCCTTCATC GCAGAAGGAG GCTGAAGGGC CTTCAAGACC CCACCGCATC CGCCGTGGCC GTAGCGGCCG TTTAAAAAAC TGTTAACTTG CACAAATAAA ATCTTAACGC TGACCGCTAC TCGCCACGTT GATTTAGTGC GTGGGCCATC ATAGTGGACT ATTTATAAGG AATTTAACGC AAATGTGCGC CATGAGACAA TCAACATTTC TCACCCAGAA TTACATCGAA TTTTCCAATG CGCCGGGCAA CTCACCAGTC TGCCATAACC GAAGGAGCTA GGAACCGGAG AATGGCAACA ACAATTAATA TCCGGCTGGC CATTGCAGCA GAGTCAGGCA TAAGCATTGG TCATTTTTAA CCCTTAACGT TTCTTGAGAT ACCAGCGGTG CTTCAGCAGA CTTCAAGAAC TGCTGCCAGT TAAGGCGCAG GACCTACACC AGGGAGAAAG GGAGCTTCCA ACTTGAGCGT CAACGCGGCC TGCGTTATCC ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ATTCTGCAGT GGCGAGGAGA GGCAACGTGA GGCACCAACA ATTCTGACCA CCCAACTACT GAGGACAAGG TACGAGATAC ACCACCGGCT GACGTCAAGA ATCTACAGGG GAGATCCTGA CGCAACTCCA CGACTCTAGA CTCCCACACC TTTATTGCAG GCATTTTTTT GTCCTGTAGC ACTTGCCAGC CGCCGGCTTT TTTACGGCAC GCCCTGATAG CTTGTTCCAA GATTTTGCCG GAATTTTAAC GGAACCCCTA TAACCCTGAT CGTGTCGCCC ACGCTGGTGA CTGGATCTCA ATGAGCACTT GAGCAACTCG ACAGAAAAGC ATGAGTGATA ACCGCTTTTT CTGAATGAAG ACGTTGCGCA GACTGGATGG TGGTTTATTG CTGGGGCCAG ACTATGGATG TAACTGTCAG TTTAAAAGGA GAGTTTTCGT CCTTTTTTTC GTTTGTTTGC GCGCAGATAC TCTGTAGCAC GGCGATAAGT CGGTCGGGCT GAACTGAGAT GCGGACAGGT GGGGGAAACG CGATTTTTGT TTTTTACGGT CCTGATTCTG AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC CGACGGTACC CCACAATGGG ATGGCCACGC CCATCAACCT CCGCGTTCAG TCAAGCAGTC GCATCGTGAA ACCTCAAGGG GGGACGCCTC TGAAGCTGCT CCAAGAAGGC ACCACGACAA CCGACGGCAT TCATAATCAG TCCCCCTGAA CTTATAATGG CACTGCATTC GGCGCATTAA GCCCTAGCGC CCCCGTCAAG CTCGACCCCA ACGGTTTTTC ACTGGAACAA ATTTCGGCCT AAAATATTAA TTTGTTTATT AAATGCTTCA TTATTCCCTT AAGTAAAAGA ACAGCGGTAA TTAAAGTTCT GTCGCCGCAT ATCTTACGGA ACACTGCGGC TGCACAACAT CCATACCAAA AACTATTAAC AGGCGGATAA CTGATAAATC ATGGTAAGCC AACGAAATAG ACCAAGTTTA TCTAGGTGAA TCCACTGAGC TGCGCGTAAT CGGATCAAGA CAAATACTGT CGCCTACATA CGTGTCTTAC GAACGGGGGG ACCTACAGCG ATCCGGTAAG CCTGGTATCT GATGCTCGTC TCCTGGCCTT TGGATAACCG TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA GCGGGCCCGG CGTAATCAAG CTTCGTGATC GGAGGTGAAG TTACGGCAAC CTTCCCCGAG GGTGAAGTCC CGAGAACTTC CACCGAGAGG GCTGGAGGGC GGTGAAGCTG GGACTACAAC GGACGAGCTG CCATACCACA CCTGAAACAT TTACAAATAA TAGTTGTGGT GCGCGGCGGG CCGCTCCTTT CTCTAAATCG AAAAACTTGA GCCCTTTGAC CACTCAACCC ATTGGTTAAA CGCTTACAAT TTTCTAAATA ATAATATTGA TTTTGCGGCA TGCTGAAGAT GATCCTTGAG GCTATGTGGC ACACTATTCT TGGCATGACA CAACTTACTT GGGGGATCAT CGACGAGCGT TGGCGAACTA AGTTGCAGGA TGGAGCCGGT CTCCCGTATC ACAGATCGCT CTCATATATA GATCCTTTTT GTCAGACCCC CTGCTGCTTG GCTACCAACT TCTTCTAGTG CCTCGCTCTG CGGGTTGGAC TTCGTGCACA TGAGCTATGA CGGCAGGGTC TTATAGTCCT AGGGGGGCGG TTGCTGGCCT TATTACCGCC

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Peroxisomes
Enzyme AatII Acc113I Acc16I Acc65I AccB1I AccB7I AccBSI AccI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII Bbv12I BcgI BcoI BglI BglII BpmI Bpu14I BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseAI BseRI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiMI BsmI BsoBI Bsp119I Bsp120I Bsp13I Bsp1407I Bsp143II Bsp19I BspCI BspEI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI Cuts 5 1 1 1 5 1 2 1 1 4 7 1 2 1 1 4 2 1 3 1 1 2 4 2 2 1 1 1 4 3 1 1 2 5 4 7 4 1 3 5 1 3 1 3 1 7 3 2 5 6 4 1 1 2 5 1 5 5 4 2 1 2 3 1 1 1 1 4 3 1 1 2 1 2 1 2 4 1 4 2 3 1 2 5 1 4 2 5 1 7 1 11 1 Positions 125 178 261 447 1175 2539 2797 645 465 645 811 1836 3067 1118 1736 2181 640 1387 629 1575 2060 2071 122 175 258 444 1124 1172 2480 596 1647 3908 666 3020 620 2352 2437 3598 2348 3594 3499 613 656 955 596 657 2348 3594 629 1575 2060 2071 7 2845 7 2845 2420 645 3020 620 2352 2437 3598 613 656 955 2797 764 660 1390 465 645 811 1836 3067 620 657 849 1806 122 175 258 444 1124 1172 2480 620 2352 2437 3598 2504 613 656 955 90 212 283 1246 2902 609 834 1197 2935 627 340 1146 1877 1423 122 175 258 444 1124 1172 2480 702 1113 2953 1511 1610 671 1408 2502 2651 3574 600 666 1387 2724 3555 3702 666 1114 1772 2935 1423 1387 699 1038 671 1408 2502 2651 3574 1423 465 645 811 1836 3067 671 1408 2502 2651 3574 620 2352 2437 3598 2356 3740 1387 1511 1610 613 656 955 627 653 1387 1379 598 1722 1730 3668 360 677 1028 2651 1387 2180 3188 3908 1736 2181 1423 2784 2966 666 1114 1772 2935 1379 666 1114 1772 2935 2356 3740 360 677 1028 627 1736 2181 360 649 677 1028 1343 1323 598 1722 1730 3668 660 1390 671 1408 2502 2651 3574 1323 634 1233 1399 1654 2774 3452 3643 340 585 609 660 1292 1390 2373 2390 3158 3170 3256 3267 828 Sequence gacgt/c agt/act tgc/gca g/gtacc g/gyrcc ccannnn/ntgg gagcgg gt/mkac t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg agc/gct gggcc/c g/tgcac r/aatty at/taat at/taat gaann/nnttc g/gtacc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc gwgcw/c cgannnnnntgc c/ycgrg gccnnnn/nggc a/gatct ctggag tt/cgaa yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc t/ccgga gaggag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg t/ccgga gaatgc c/ycgrg tt/cgaa g/ggccc t/ccgga t/gtaca rgcgc/y c/catgg cgat/cg t/ccgga t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg BstYI BstZI CciNI Cfr10I Cfr42I Cfr9I CfrI Csp45I DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco88I Eco91I EcoICRI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I ErhI Esp1396I FauNDI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I Kpn2I KpnI Ksp632I KspI LspI MamI MfeI MflI MluI MluNI Mph1103I MroI MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI NspV PaeR7I PflMI PinAI Ple19I Ppu10I PshBI Psp124BI Psp1406I PspAI PspALI PspEI PspOMI PstI 11 1 1 4 1 1 4 1 4 1 2 2 5 4 1 1 1 1 1 1 1 1 3 4 1 3 1 1 4 5 3 1 1 1 1 1 3 1 3 1 1 4 1 3 4 7 3 3 1 1 7 1 1 1 1 1 1 1 11 1 1 1 1 1 1 6 7 4 1 2 1 3 1 1 1 1 1 1 4 1 1 1 1 1 1 1 2 1 2 1 1 1 1 1 585 609 660 1292 1390 2373 2390 3158 3170 3256 3267 1405 1405 666 1114 1772 2935 652 656 762 1203 1405 2627 627 1463 2442 3134 3153 902 1275 1880 1924 3806 360 649 677 1028 1343 762 1203 1405 2627 1405 2231 3020 2231 618 3020 1405 340 360 677 1028 620 657 849 1806 2539 702 1113 2953 596 1405 744 1167 2338 3386 465 645 811 1836 3067 613 656 955 1323 618 902 1323 629 360 677 1028 3965 360 677 1028 1118 234 620 657 849 1806 2797 834 1197 2935 598 1722 1730 3668 122 175 258 444 1124 1172 2480 641 1218 1524 641 1218 1524 622 1524 122 175 258 444 1124 1172 2480 1387 649 2231 652 627 1423 1511 585 609 660 1292 1390 2373 2390 3158 3170 3256 3267 1647 764 3965 1387 1772 764 365 679 1027 2249 2608 2767 122 175 258 444 1124 1172 2480 651 2384 3325 3570 1511 1511 1610 1774 360 677 1028 234 1772 1772 591 1405 3965 651 2384 3325 3570 3912 627 613 1118 666 2651 3961 7 2845 620 2418 2791 656 658 1323 653 638 r/gatcy c/ggccg gc/ggccgc r/ccggy ccgc/gg c/ccggg y/ggccr tt/cgaa ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc c/ycgrg g/gtnacc gag/ctc rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc t/ccgga ggtac/c ctcttc ccgc/gg tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa c/tcgag ccannnn/ntgg a/ccggt cgat/cg a/tgcat at/taat gagct/c aa/cgtt c/ccggg ccc/ggg g/gtnacc g/ggccc ctgca/g

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Peroxisomes
PstNHI PvuI RcaI SacI SacII SalI ScaI SfcI Sfr274I Sfr303I SfuI SmaI SnaBI SspBI 1 1 2 1 1 1 1 7 1 1 1 1 1 1 591 2651 2180 3188 620 652 639 2539 634 1233 1399 1654 2774 3452 3643 613 652 627 658 340 1379 g/ctagc cgat/cg t/catga gagct/c ccgc/gg g/tcgac agt/act c/tryag c/tcgag ccgc/gg tt/cgaa ccc/ggg tac/gta t/gtaca SspI SstI SstII StyI Van91I VneI VspI XbaI XcmI XhoI XhoII XmaI XmaIII XmnI Zsp2I 2 1 1 3 1 2 2 1 1 1 11 1 1 1 1 2085 2215 620 652 360 677 1028 1118 2348 3594 7 2845 1415 1365 613 585 609 660 1292 1390 2373 2390 3158 3170 3256 3267 656 1405 2420 3965 aat/att gagct/c ccgc/gg c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy c/ccggg c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: AatI, AclNI, AflII, AocI, AscI, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbsI, BbuI, Bbv16II, BclI, BfrI, BlnI, BlpI, BpiI, Bpu1102I, BpuAI, Bsa29I, BscI, Bse21I, BseCI, BsePI, BsgI, BsiWI, BsmBI, Bsp106I, Bsp1720I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, Bsu15I, Bsu36I, CelII, ClaI, CpoI, CspI, CvnI, Eco147I, Eco32I, Eco72I, Eco81I, EcoNI, EcoRV, EheI, Esp3I, FbaI, FseI, KasI, Ksp22I, MspCI, NarI, NruI, PacI, PaeI, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp5II, PspLI, PvuII, RsrII, SapI, SbfI, SexAI, SfiI, SgfI, SgrAI, SmiI, SpeI, SphI, SplI, SrfI, Sse8387I, SseBI, StuI, SunI, SwaI, Tth111I, Vha464I

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010