Alleleustrious pmWasabi-Tubulin

Catalog Number: Introduction All ustrious pmWasabi-Tubulin is a mammalian expression vector that expresses mWasabi fused to human alpha tubulin 1b (TUBA1B). This product can be a great tool for your research involving cytoskeleton (microtubules). All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. Tubulins are globular proteins that make up microtubules. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Each tubulin protein has a molecular weight of approximately 55 kD. Microtubules are assembled from dimers of alpha- and beta-tubulin. Gamma-tubulin, another member of the tubulin family, is important in the nucleation and polar orientation of microtubules and found primarily in centrosomes and spindle pole bodies.
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ABP-FP-WTUB

Source Engineered variant of mTFP1, originally derived from Clavularia sp. coral. Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Wasabi.613-1320 Tubulin....1321-2694 SV40 PolyA Signal.....................2854-2904 bla Promoter...............................3443-3547 Ampicillin Resistance Gene......3532-4392 pUC Origin..................................4541-5183 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A Tubulin AMPR PCMV pUC Ori

pmWasabiTubulin 5265bp

Wasabi

Linker

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Dutcher SK (2001). "The tubulin fraternity: alpha to eta" Curr. Opin. Cell Biol. 13 (1): 4954. Hall JL, Cowan NJ (1985). "Structural features and restricted expression of a human alpha-tubulin gene" Nucleic Acids Res. 13 (1): 20723.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Tubulin
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGTCGCCA ATGAAGATCA GAGGGCGAGG GCCCCCCTGC TTCACCAAGT TCTTGGGAGC TCCATGGAGG AACGGCCCCG GTGCGCGACG CACCACCGCG TATCACTTTG ACCGTTTACG TCCGGACTCA CAGATTGGCA CAGATGCCAA GAGACGGGCG ATTGATGAAG GGCAAGGAAG ATTGACCTTG TTCTTGGTTT GAACGTCTCT CCCCAGGTTT CTGGAGCACT AGAAACCTCG GTGTCCTCCA TTCCAGACCA GTCATCTCTG TGCTTTGAGC TGCTGCCTGT ATCAAAACCA ATCAACTACC GTGTGCATGC TTTGACCTGA GAAGGCGAGT GTTGGTGTGG ACCGGATCTA TTAAAAAACC GTTAACTTGT ACAAATAAAG TCTTAACGCG GACCGCTACA CGCCACGTTC ATTTAGTGCT TGGGCCATCG TAGTGGACTC TTTATAAGGG ATTTAACGCG AATGTGCGCG ATGAGACAAT CAACATTTCC CACCCAGAAA TACATCGAAC TTTCCAATGA GCCGGGCAAG TCACCAGTCA GCCATAACCA AAGGAGCTAA GAACCGGAGC ATGGCAACAA CAATTAATAG CCGGCTGGCT ATTGCAGCAC AGTCAGGCAA AAGCATTGGT TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA CCATGGTGAG AGCTGAAGAT GCAAGCCCTA CCTTCTCCTA ACCCCGACGA GCACCATGAC AGGACTCCTT TGATGCAGAA GCGTGCTGAA TTGACTTCAA TGGACCACCG AGATCGCCGT GATCTCGAGT ATGCCTGCTG GTGACAAGAC CTGGCAAGCA TTCGCACTGG ATGCTGCCAA TGTTGGACCG TCCACAGCTT CAGTTGATTA CCACAGCTGT CTGATTGTGC ATATCGAGCG TCACTGCTTC ACCTGGTGCC CTGAGAAAGC CAGCCAACCA TGTACCGTGG AGCGCAGCAT AGCCTCCCAC TGAGCAACAC TGTATGCCAA TTTCAGAGGC ATTCTGTTGA GATAACTGAT TCCCACACCT TTATTGCAGC CATTTTTTTC TCCTGTAGCG CTTGCCAGCG GCCGGCTTTC TTACGGCACC CCCTGATAGA TTGTTCCAAA ATTTTGCCGA AATTTTAACA GAACCCCTAT AACCCTGATA GTGTCGCCCT CGCTGGTGAA TGGATCTCAA TGAGCACTTT AGCAACTCGG CAGAAAAGCA TGAGTGATAA CCGCTTTTTT TGAATGAAGC CGTTGCGCAA ACTGGATGGA GGTTTATTGC TGGGGCCAGA CTATGGATGA AACTGTCAGA TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGGGCGAG GGAGGGCAAC CGACGGCACC CGACATTCTG CATCCCCAAC CTTCGAGGAC CATCTACGAG GGAGACCACC GGGCGACGTC GACCATCTAC CATCGAGATC GGCCCGCAAC GCGTGAGTGC GGAGCTCTAC CATTGGGGGA CGTGCCCCGG CACCTACCGC TAACTATGCC AATTCGCAAG TGGTGGGGGA TGGCAAGAAG AGTTGAGCCC CTTCATGGTA CCCAACCTAC CCTGAGATTT CTACCCCCGC CTACCATGAA GATGGTGAAA TGACGTGGTT CCAGTTTGTG TGTGGTGCCT CACAGCCATT GCGTGCCTTT CCGTGAAGAT AGGAGAGGGT CATAATCAGC CCCCCTGAAC TTATAATGGT ACTGCATTCT GCGCATTAAG CCCTAGCGCC CCCGTCAAGC TCGACCCCAA CGGTTTTTCG CTGGAACAAC TTTCGGCCTA AAATATTAAC TTGTTTATTT AATGCTTCAA TATTCCCTTT AGTAAAAGAT CAGCGGTAAG TAAAGTTCTG TCGCCGCATA TCTTACGGAT CACTGCGGCC GCACAACATG CATACCAAAC ACTATTAACT GGCGGATAAA TGATAAATCT TGGTAAGCCC ACGAAATAGA CCAAGTTTAC ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC GAGACCACAA GTGAATGGCC AACACCATCA ACCACCGCGT TACTTCAAGC AAGGGCATCG ATACACCTCA GGCTGGGACG AAGATGAAGC AGGGCCAAGA CTGAACCACG TCCACCGACG ATCTCCATCC TGCCTGGAAC GGAGATGACT GCTGTGTTTG CAGCTCTTCC CGAGGGCACT CTGGCTGACC ACTGGTTCTG TCCAAGCTGG TACAACTCCA GACAATGAGG ACTAACCTTA GATGGAGCCC ATCCACTTCC CAGCTTTCTG TGTGACCCTC CCCAAAGATG GATTGGTGCC GGTGGAGACC GCTGAGGCCT GTTCACTGGT ATGGCTGCCC GAGGAAGAAG CATACCACAT CTGAAACATA TACAAATAAA AGTTGTGGTT CGCGGCGGGT CGCTCCTTTC TCTAAATCGG AAAACTTGAT CCCTTTGACG ACTCAACCCT TTGGTTAAAA GCTTACAATT TTCTAAATAC TAATATTGAA TTTGCGGCAT GCTGAAGATC ATCCTTGAGA CTATGTGGCG CACTATTCTC GGCATGACAG AACTTACTTC GGGGATCATG GACGAGCGTG GGCGAACTAC GTTGCAGGAC GGAGCCGGTG TCCCGTATCG CAGATCGCTG TCATATATAC AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC TGGGCGTAAT ACGCCTTCGT ACCTGGAGGT TCAGTTACGG AGTCCTTCCC TGAAGGTGAA AGGGCGAGAA CCTCCACCGA TGCTGCTGGA AGGCGGTGAA ACAAGGACTA GCATGGACGA ACGTTGGCCA ACGGCATCCA CCTTCAACAC TAGACTTGGA ACCCTGAGCA ACACCATTGG AGTGCACCGG GGTTCACCTC AGTTCTCCAT TCCTCACCAC CCATCTATGA ACCGCCTTAT TGAATGTTGA CTCTGGCCAC TAGCAGAGAT GCCATGGTAA TCAATGCTGC CCACTGGCTT TGGCCAAGGT GGGCTCGCCT ACGTGGGTGA TTGAGAAGGA GAGAGGAATA TTGTAGAGGT AAATGAATGC GCAATAGCAT TGTCCAAACT GTGGTGGTTA GCTTTCTTCC GGGCTCCCTT TAGGGTGATG TTGGAGTCCA ATCTCGGTCT AATGAGCTGA TAGGTGGCAC ATTCAAATAT AAAGGAAGAG TTTGCCTTCC AGTTGGGTGC GTTTTCGCCC CGGTATTATC AGAATGACTT TAAGAGAATT TGACAACGAT TAACTCGCCT ACACCACGAT TTACTCTAGC CACTTCTGCG AGCGTGGGTC TAGTTATCTA AGATAGGTGC TTTAGATTGA TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CAAGCCCGAC GATCGAGGGC GAAGGAGGGA CAACAGGGCC CGAGGGCTAC GTCCGACATC CTTCCCCCCC GAGGATGTAC GGGCGGCGGC GCTGCCCGAC CAACAAGGTG GCTGTACAAG GGCTGGTGTC GCCCGATGGC CTTCTTCAGT ACCCACAGTC GCTCATCACA CAAGGAGATC TCTTCAGGGC CCTGCTCATG TTACCCAGCA CCACACCACC CATCTGTCGT TAGCCAGATT CCTGACAGAA ATATGCCCCT CACCAATGCT ATACATGGCT CATTGCCACC CAAGGTTGGC ACAGAGAGCT GGACCACAAG GGGGATGGAG TTATGAGGAG CTAAGGATCC TTTACTTGCT AATTGTTGTT CACAAATTTC CATCAATGTA CGCGCAGCGT CTTCCTTTCT TAGGGTTCCG GTTCACGTAG CGTTCTTTAA ATTCTTTTGA TTTAACAAAA TTTTCGGGGA GTATCCGCTC TATGAGTATT TGTTTTTGCT ACGAGTGGGT CGAAGAACGT CCGTATTGAC GGTTGAGTAC ATGCAGTGCT CGGAGGACCG TGATCGTTGG GCCTGTAGCA TTCCCGGCAA CTCGGCCCTT TCGCGGTATC CACGACGGGG CTCACTGATT TTTAAAACTT

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Tubulin
4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 CATTTTTAAT CCTTAACGTG TCTTGAGATC CCAGCGGTGG TTCAGCAGAG TTCAAGAACT GCTGCCAGTG AAGGCGCAGC ACCTACACCG GGGAGAAAGG GAGCTTCCAG CTTGAGCGTC AACGCGGCCT GCGTTATCCC TTAAAAGGAT AGTTTTCGTT CTTTTTTTCT TTTGTTTGCC CGCAGATACC CTGTAGCACC GCGATAAGTC GGTCGGGCTG AACTGAGATA CGGACAGGTA GGGGAAACGC GATTTTTGTG TTTTACGGTT CTGATTCTGT CTAGGTGAAG CCACTGAGCG GCGCGTAATC GGATCAAGAG AAATACTGTT GCCTACATAC GTGTCTTACC AACGGGGGGT CCTACAGCGT TCCGGTAAGC CTGGTATCTT ATGCTCGTCA CCTGGCCTTT GGATAACCGT ATCCTTTTTG TCAGACCCCG TGCTGCTTGC CTACCAACTC CTTCTAGTGT CTCGCTCTGC GGGTTGGACT TCGTGCACAC GAGCTATGAG GGCAGGGTCG TATAGTCCTG GGGGGGCGGA TGCTGGCCTT ATTACCGCCA ATAATCTCAT TAGAAAAGAT AAACAAAAAA TTTTTCCGAA AGCCGTAGTT TAATCCTGTT CAAGACGATA AGCCCAGCTT AAAGCGCCAC GAACAGGAGA TCGGGTTTCG GCCTATGGAA TTGCTCACAT TGCAT GACCAAAATC CAAAGGATCT ACCACCGCTA GGTAACTGGC AGGCCACCAC ACCAGTGGCT GTTACCGGAT GGAGCGAACG GCTTCCCGAA GCGCACGAGG CCACCTCTGA AAACGCCAGC GTTCTTTCCT

Enzyme AatII AatI AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I Aor51HI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbrPI BbsI BbuI Bbv12I Bbv16II BcgI BclI BcoI BglI BglII BlpI BpiI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseAI BseRI Bsh1285I Bsh1365I BshNI

Cuts 5 1 5 1 1 8 2 2 2 1 5 7 1 2 2 1 6 3 1 4 1 3 5 2 2 2 1 6 4 1 5 1 8 6 7 1 1 1 6 1 1 1 4 6 1 1 1 5 1 1 5 1 7 4 2 4 7 5 1 1 4 4 1 8

Positions 125 178 261 447 1175 2497 125 178 261 447 1109 3838 4096 465 745 1579 2115 2375 2424 3135 4366 1052 2367 3035 3480 1541 1949 1321 1700 2098 2874 3359 3370 122 175 258 444 1058 1106 3779 596 2946 5207 600 1726 4319 1406 1726 1928 3651 3736 4897 1722 3647 4893 4798 889 1334 1526 1649 596 1722 3647 4893 1700 2098 2874 3359 3370 7 4144 7 4144 1773 3719 4319 1406 1726 1928 3651 3736 4897 889 1334 1526 1649 4096 698 1367 1439 2146 2443 2695 465 745 1579 2115 2375 2424 3135 4366 783 1406 1889 2079 2505 3105 122 175 258 444 1058 1106 3779 1521 1735 2469 1406 1726 1928 3651 3736 4897 1735 3803 2717 889 1334 1526 1649 90 212 283 1180 2384 4201 1330 2470 1735 768 1131 1842 1926 4234 2470 1735 340 1080 1521 2562 3176 2722 122 175 258 444 1058 1106 3779 636 1047 2440 4252 2810 2909 605 3801 3950 4873 600 1321 1726 2701 4023 4854 5001 600 1048 1726 3071 4234 2722 1321 633 972 1474 2640 605 3801 3950 4873 2722 465 745 1579 2115 2375 2424 3135

Sequence gacgt/c agg/cct gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg gt/mkac t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg agc/gct g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc cac/gtg gaagac gcatg/c gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc a/gatct gc/tnagc gaagac ctggag gc/tnagc gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc t/ccgga gaggag cgry/cg gatnn/nnatc g/gyrcc

BsiEI BsiHKAI BsiI BsiMI BsmBI BsmI BsoBI Bsp13I Bsp1407I Bsp143II Bsp1720I Bsp19I BspCI BspEI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI CelII Cfr10I Cfr9I CfrI DraI DraII DraIII DrdI DsaI EaeI Eam1104I Eam1105I EarI Ecl136II EclHKI Eco105I Eco130I Eco147I Eco24I Eco255I Eco31I Eco32I Eco47III Eco57I Eco64I

4 6 2 1 2 2 4 1 1 6 1 4 1 1 2 1 2 1 5 5 1 5 2 5 2 6 1 6 1 4 1 7 1 11 4 11 1 5 1 7 4 1 2 2 6 7 2 1 2 1 1 1 5 1 6 1 4 1 1 6 8

4366 605 3801 3950 4873 1406 1726 1928 3651 3736 4897 3655 5039 1321 1506 1809 2810 2909 889 1334 1526 1649 1321 1313 598 1511 2001 3021 3029 4967 2470 360 611 962 2262 3950 1321 3479 4487 5207 3035 3480 2722 1393 2336 2492 4083 4265 600 1048 1726 3071 4234 1313 600 1048 1726 3071 4234 3655 5039 360 611 962 2262 2444 3035 3480 360 611 962 1277 2262 2295 1257 598 1511 2001 3021 3029 4967 2695 605 3801 3950 4873 1257 1167 1877 2198 2953 4073 4751 4942 340 585 1226 1330 2695 2704 3672 3689 4457 4469 4555 4566 762 1435 1759 2241 585 1226 1330 2695 2704 3672 3689 4457 4469 4555 4566 2470 600 1048 1726 3071 4234 1526 696 1137 1365 1437 2144 2441 3926 2762 3741 4433 4452 836 1209 3179 3223 5105 360 611 962 1277 2262 2295 696 1137 1365 1437 2144 2441 3926 1599 3530 4319 1599 3530 1404 4319 340 360 611 962 2262 2444 2497 783 1406 1889 2079 2505 3105 3838 636 1047 2440 4252 1992 596 678 1101 1499 1737 3637 4685 465 745 1579 2115 2375 2424 3135 4366

cgry/cg gwgcw/c ctcgtg t/ccgga cgtctc gaatgc c/ycgrg t/ccgga t/gtaca rgcgc/y gc/tnagc c/catgg cgat/cg t/ccgga t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy gc/tnagc r/ccggy c/ccggg y/ggccr ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc tac/gta c/cwwgg agg/cct grgcy/c agt/act ggtctc gat/atc agc/gct ctgaag g/gyrcc

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Tubulin
Eco72I Eco88I Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I ErhI Esp1396I Esp3I FauNDI FbaI FriOI FspI GsuI HaeII Hin1I HincII HindII HpaI Hsp92I Kpn2I Ksp22I Ksp632I MamI MfeI MflI MluI MluNI Mph1103I MroI MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI 1 4 1 1 2 1 1 2 1 5 1 5 2 2 2 1 6 1 5 6 7 3 3 1 7 1 1 2 1 1 11 1 5 1 1 1 5 7 7 4 1 2 1 4 2 1 1 1 1521 889 1334 1526 1649 1257 1404 2443 2623 836 1257 1700 2098 1992 360 611 962 2262 2444 5264 360 611 962 2262 2444 1052 2367 1506 1809 234 2151 2717 783 1406 1889 2079 2505 3105 4096 768 1131 1842 1926 4234 598 1511 2001 3021 3029 4967 122 175 258 444 1058 1106 3779 1152 2088 2823 1152 2088 2823 2823 122 175 258 444 1058 1106 3779 1321 2717 1599 3530 2722 2810 585 1226 1330 2695 2704 3672 3689 4457 4469 4555 4566 2946 698 1367 1439 2146 2443 5264 1321 3071 698 1367 1439 2146 2443 365 613 961 2422 3548 3907 4066 122 175 258 444 1058 1106 3779 1876 3683 4624 4869 2810 2810 2909 3073 360 611 962 2262 234 2151 3071 3071 591 cac/gtg c/ycgrg g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc gat/atc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg cgtctc ca/tatg t/gatca grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac gtt/aac gr/cgyc t/ccgga t/gatca ctcttc gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc NsiI NspBII NspI PaeI PaeR7I PflMI PinAI Ple19I PmaCI Pme55I PmlI Ppu10I PshAI PshBI Psp124BI Psp1406I PspAI PspALI PspEI PstNHI PvuI PvuII RcaI SacI SapI ScaI SexAI SfcI Sfr274I SmaI SnaBI SphI SseBI SspBI SspI SstI StuI StyI Van91I VneI VspI XbaI XcmI XhoI XhoII XmaI XmnI Zsp2I 1 4 2 1 1 2 2 1 1 1 1 1 1 2 1 2 1 1 1 1 1 1 2 1 1 1 1 7 1 1 1 1 1 1 2 1 1 5 2 3 2 1 2 1 11 1 2 1 5264 1876 3683 4624 4869 2469 5211 2469 1334 1052 2367 600 1726 3950 1521 2497 1521 5260 1973 7 4144 1406 3717 4090 1526 1528 1257 591 3950 1876 3479 4487 1406 1599 3838 2111 1167 1877 2198 2953 4073 4751 4942 1334 1528 340 2469 2497 1313 3384 3514 1406 2497 360 611 962 2262 2444 1052 2367 1722 3647 4893 7 4144 2707 1299 1759 1334 585 1226 1330 2695 2704 3672 3689 4457 4469 4555 4566 1526 1773 3719 5264 atgca/t cmg/ckg rcatg/y gcatg/c c/tcgag ccannnn/ntgg a/ccggt cgat/cg cac/gtg agg/cct cac/gtg a/tgcat gacnn/nngtc at/taat gagct/c aa/cgtt c/ccggg ccc/ggg g/gtnacc g/ctagc cgat/cg cag/ctg t/catga gagct/c gctcttc agt/act a/ccwggt c/tryag c/tcgag ccc/ggg tac/gta gcatg/c agg/cct t/gtaca aat/att gagct/c agg/cct c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy c/ccggg gaann/nnttc atgca/t

The following enzymes do not cut: Acc65I, AclNI, AflII, AocI, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BfrI, BlnI, Bpu14I, Bsa29I, BscI, Bse21I, BseCI, BsePI, BsgI, BsiWI, Bsp106I, Bsp119I, Bsp120I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstBI, BstZI, Bsu15I, Bsu36I, CciNI, Cfr42I, ClaI, CpoI, Csp45I, CspI, CvnI, EagI, EclXI, Eco52I, Eco81I, EheI, FseI, HindIII, KasI, KpnI, KspI, LspI, MspCI, NarI, NotI, NruI, NspV, PacI, Pfl23II, PmeI, PpuMI, Psp5II, PspLI, PspOMI, PstI, RsrII, SacII, SalI, SbfI, SfiI, Sfr303I, SfuI, SgfI, SgrAI, SmiI, SpeI, SplI, SrfI, Sse8387I, SstII, SunI, SwaI, Tth111I, Vha464I, XmaIII

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Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party.

Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010