Alleleustrious pmTFP1-Cx32

Catalog Number: Introduction All ustrious pmTFP1-Cx32 is a mammalian expression vector that encodes a fusion of human beta-1 connexin-32 (protein Cx32, gene GJB1) N-terminal to mTFP1. It can be a great tool for your research on gap junctions. The properties of All ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in the mitotic spindle of mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. Connexins are gap junction proteins that assemble to form 6 member hemichannels in vertebrates. These structures can move around freely within the lipid bilayer and with help from specific proteins, mainly cadherins, the hemichannels are able to dock with hemichannels of adjacent cells forming gap junctions. Connexin protein family members are diverse, Cx32 refers to the protein with 32 kD molecular weight. It is reported to be a tumor suppressor gene in human renal cell carcinoma (most chemoresistant cancer).
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ABP-FP-TCX32

Source Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features More than 3-fold brighter than ECFP More than twice as photostable as ECFP Single emission peak Single-exponential fluorescence lifetime ( = 3.2 ns) Can be co-imaged with red and yellow FPs Low sensitivity to acidic pH (fluorescence pKa=4.3) Mammalian expression vector ready to transfect your favorite cells True monomer that will not aggregate or cause non-specific interactions Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Cx32............................................697-1545 Linker......1546-1566 mTFP1.1567-2277 SV40 PolyA Signal.....................2429-2479 bla Promoter...............................3018-3122 Ampicillin Resistance Gene......3107-3967 pUC Origin..................................4116-4758 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A mTFP1 AMPR pUC Ori PCMV

pmTFP1-Cx32 4840bp

Cx32

Linker

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Sato H, Hagiwara H, Ohde Y, et al.. "Regulation of renal cell carcinoma cell proliferation, invasion and metastasis by connexin 32 gene.". J. Membr. Biol. 2007 216 (1): 1721. Aronica E, Gorter J, Jansen G et al. "Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex". Acta Neuropathol. 2001 101 (5): 44959.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Cx32
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG GGTGTGGCAG TTGCTCAGTG TTCATCTTCA TCTTCCTTCA TTCCCCATCT CTCCTCGTGG GAGGGCCATG GGGACACTGT TTCATGTATG GACGTCTACC GTCTTCACCG GTGGTGTACC TCCCGCAAGG AACAAGCTGC ACCGGGGCTG GCCACCATGG ATCAAGCTGA GAGGGCAAGC CTGCCCTTCT AAGTACCCCG GAGCGCACCA GAGGAGGACT CCCGTGATGC GACGGCGTGC CGCGTTGACT TTTGTGGACC TACGAGAGCG GCCGCGACTC AAACCTCCCA CTTGTTTATT TAAAGCATTT ACGCGTCCTG CTACACTTGC CGTTCGCCGG GTGCTTTACG CATCGCCCTG GACTCTTGTT AAGGGATTTT ACGCGAATTT GCGCGGAACC ACAATAACCC TTTCCGTGTC AGAAACGCTG CGAACTGGAT AATGATGAGC GCAAGAGCAA AGTCACAGAA AACCATGAGT GCTAACCGCT GGAGCTGAAT AACAACGTTG AATAGACTGG TGGCTGGTTT AGCACTGGGG GGCAACTATG TTGGTAACTG TTAATTTAAA ACGTGAGTTT AGATCCTTTT GGTGGTTTGT CAGAGCGCAG GAACTCTGTA CAGTGGCGAT TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT TGACAGGGAG GCGTGAACCG GAATCATGGT TCTGCAACAC CCCATGTGCG CCATGCACGT GGGACCCCCT GGTGGACCTA TCTTTTATCT CCTGCCCCAA TCTTCATGCT TCATCATCCG GCTCGGGCTT TGAGTGAGCA GGCTGGCTGA TGAGCAAGGG AGATGGAGGG CCTACGACGG CCTACGACAT ACGACATCCC TGACCTTCGA CCTTCATCTA AGAAGAAGAC TGAAGGGCGA TCAAGACCAT ACCGCATCGA CCGTGGCCCG TAGATCATAA CACCTCCCCC GCAGCTTATA TTTTCACTGC TAGCGGCGCA CAGCGCCCTA CTTTCCCCGT GCACCTCGAC ATAGACGGTT CCAAACTGGA GCCGATTTCG TAACAAAATA CCTATTTGTT TGATAAATGC GCCCTTATTC GTGAAAGTAA CTCAACAGCG ACTTTTAAAG CTCGGTCGCC AAGCATCTTA GATAACACTG TTTTTGCACA GAAGCCATAC CGCAAACTAT ATGGAGGCGG ATTGCTGATA CCAGATGGTA GATGAACGAA TCAGACCAAG AGGATCTAGG TCGTTCCACT TTTCTGCGCG TTGCCGGATC ATACCAAATA GCACCGCCTA AAGTCGTGTC TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGA GTGTGAATGA GCATTCTACT GCTGGTGGTG ACTCCAGCCT GCTGTGGTCC GGCTCACCAG ACACCTGGAG TGTCATCAGC GCTCTACCCT CACAGTGGAC AGCTGCCTCT GGCCTGTGCC CGGCCACCGC GGATGGCTCC AAAGAGCGAC CGAGGAGACC CAACGTGAAT CACCAACACC TCTGACCACC CAACTACTTC GGACAAGGGC CGAGATACAC CACCGGCTGG CGTCAAGCAC CTACAGGGCC GATCCTGAAC CAACTCCACC TCAGCCATAC TGAACCTGAA ATGGTTACAA ATTCTAGTTG TTAAGCGCGG GCGCCCGCTC CAAGCTCTAA CCCAAAAAAC TTTCGCCCTT ACAACACTCA GCCTATTGGT TTAACGCTTA TATTTTTCTA TTCAATAATA CCTTTTTTGC AAGATGCTGA GTAAGATCCT TTCTGCTATG GCATACACTA CGGATGGCAT CGGCCAACTT ACATGGGGGA CAAACGACGA TAACTGGCGA ATAAAGTTGC AATCTGGAGC AGCCCTCCCG ATAGACAGAT TTTACTCATA TGAAGATCCT GAGCGTCAGA TAATCTGCTG AAGAGCTACC CTGTTCTTCT CATACCTCGC TTACCGGGTT ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ATTCCCTCTG GGCAGGATGA GCCATTGGCC GCTGCAGAGA GGCTGCAACA CTGCAGCTCA CAACACATAG GAGGTGAAGA GTGGTGTTCC GGCTATGCCA TGCTTCGTGT GGCATCTGCA CGCCGAGCCC CTCTCACCTG CTGAAAGACA CGCTGCTCGG ACAATGGGCG GGCCACGCCT ATCAACCTGG GCGTTCGCCT AAGCAGTCCT ATCGTGAAGG CTCAAGGGCG GACGCCTCCA AAGCTGCTGC AAGAAGGCGG CACGACAAGG GACGGCATGG CACATTTGTA ACATAAAATG ATAAAGCAAT TGGTTTGTCC CGGGTGTGGT CTTTCGCTTT ATCGGGGGCT TTGATTAGGG TGACGTTGGA ACCCTATCTC TAAAAAATGA CAATTTAGGT AATACATTCA TTGAAAAAGG GGCATTTTGC AGATCAGTTG TGAGAGTTTT TGGCGCGGTA TTCTCAGAAT GACAGTAAGA ACTTCTGACA TCATGTAACT GCGTGACACC ACTACTTACT AGGACCACTT CGGTGAGCGT TATCGTAGTT CGCTGAGATA TATACTTTAG TTTTGATAAT CCCCGTAGAA CTTGCAAACA AACTCTTTTT AGTGTAGCCG TCTGCTAATC GGACTCAAGA AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC GGAAAGGGCA ACTGGACAGG GAGTATGGCT GTGTGTGGGG GCGTTTGCTA TCCTAGTTTC AGAAGAAAAT GGCACAAGGT GGCTGTTGTT TGGTGCGGCT CCCGCCCCAC TCATCCTCAA AGCGCCGCTC AATACAAGCA TACTGCGCCG CCTGCGCGGA TAATCAAGCC TCGTGATCGA AGGTGAAGGA ACGGCAACAG TCCCCGAGGG TGAAGTCCGA AGAACTTCCC CCGAGAGGAT TGGAGGGCGG TGAAGCTGCC ACTACAACAA ACGAGCTGTA GAGGTTTTAC AATGCAATTG AGCATCACAA AAACTCATCA GGTTACGCGC CTTCCCTTCC CCCTTTAGGG TGATGGTTCA GTCCACGTTC GGTCTATTCT GCTGATTTAA GGCACTTTTC AATATGTATC AAGAGTATGA CTTCCTGTTT GGTGCACGAG CGCCCCGAAG TTATCCCGTA GACTTGGTTG GAATTATGCA ACGATCGGAG CGCCTTGATC ACGATGCCTG CTAGCTTCCC CTGCGCTCGG GGGTCTCGCG ATCTACACGA GGTGCCTCAC ATTGATTTAA CTCATGACCA AAGATCAAAG AAAAAACCAC CCGAAGGTAA TAGTTAGGCC CTGTTACCAG CGATAGTTAC TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA GCAGCAGCCA TTTGTACACC CTCGGTCATC TGATGAGAAA TGACCAATTC CACCCCAGCT GCTACGGCTT CCACATCTCA TGAGGCCGTC GGTCAAGTGC CGAGAAAACC TGTGGCCGAG CAATCCACCT GAATGAGATC CAGCCCTGGC TCCACCGGTC CGACATGAAG GGGCGAGGGC GGGAGCCCCC GGCCTTCACC CTACTCTTGG CATCTCCATG CCCCAACGGC GTACGTGCGC CGGCCACCAC CGACTATCAC GGTGACCGTT CAAGTAAGCG TTGCTTTAAA TTGTTGTTAA ATTTCACAAA ATGTATCTTA AGCGTGACCG TTTCTCGCCA TTCCGATTTA CGTAGTGGGC TTTAATAGTG TTTGATTTAT CAAAAATTTA GGGGAAATGT CGCTCATGAG GTATTCAACA TTGCTCACCC TGGGTTACAT AACGTTTTCC TTGACGCCGG AGTACTCACC GTGCTGCCAT GACCGAAGGA GTTGGGAACC TAGCAATGGC GGCAACAATT CCCTTCCGGC GTATCATTGC CGGGGAGTCA TGATTAAGCA AACTTCATTT AAATCCCTTA GATCTTCTTG CGCTACCAGC CTGGCTTCAG ACCACTTCAA TGGCTGCTGC CGGATAAGGC

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Cx32
4441 4501 4561 4621 4681 4741 4801 GCAGCGGTCG CACCGAACTG AAAGGCGGAC TCCAGGGGGA GCGTCGATTT GGCCTTTTTA ATCCCCTGAT GGCTGAACGG AGATACCTAC AGGTATCCGG AACGCCTGGT TTGTGATGCT CGGTTCCTGG TCTGTGGATA GGGGTTCGTG AGCGTGAGCT TAAGCGGCAG ATCTTTATAG CGTCAGGGGG CCTTTTGCTG ACCGTATTAC CACACAGCCC ATGAGAAAGC GGTCGGAACA TCCTGTCGGG GCGGAGCCTA GCCTTTTGCT CGCCATGCAT AGCTTGGAGC GCCACGCTTC GGAGAGCGCA TTTCGCCACC TGGAAAAACG CACATGTTCT GAACGACCTA CCGAAGGGAG CGAGGGAGCT TCTGACTTGA CCAGCAACGC TTCCTGCGTT

Enzyme AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccI AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I Aor51HI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbrPI BbsI Bbv12I Bbv16II BcgI BcoI BglI BglII BpiI BpmI Bpu14I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseRI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsmI BsoBI Bsp119I Bsp1407I Bsp143II Bsp19I BspCI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I

Cuts 6 1 1 5 1 3 1 4 8 1 2 1 1 4 2 1 3 1 2 4 2 2 1 1 4 3 1 2 1 5 5 8 1 4 4 4 1 3 5 1 4 5 1 4 4 1 8 2 3 6 5 5 1 4 6 1 5 6 4 3 3 3 1 2 6 5 1 2 1 3 1 2 5 2 5 3 5 1 3 6 1 6

Positions 125 178 261 447 1205 2063 3413 3671 465 1498 1699 2710 3941 2006 1370 2610 3055 1205 629 2449 2934 2945 122 175 258 444 1202 2012 2060 3354 596 2521 4782 1554 3894 620 3226 3311 4472 3222 4468 4373 613 1392 1843 596 3222 4468 629 2449 2934 2945 7 3719 7 3719 3294 3894 620 3226 3311 4472 613 1392 1843 3671 970 1652 1548 465 1498 1699 2710 3941 620 1359 1393 1737 2680 122 175 258 444 1202 2012 2060 3354 978 1143 1266 1275 2000 620 3226 3311 4472 1143 1266 1275 2000 3378 613 1392 1843 90 212 283 2134 3776 609 1143 1266 1275 2000 867 1050 1722 2085 3809 627 1143 1266 1275 2000 340 978 2034 2751 2297 122 175 258 444 1202 2012 2060 3354 1590 3827 746 2385 2484 1530 1559 2282 3376 3525 4448 600 1554 3598 4429 4576 737 1554 2002 2646 3809 2297 966 1053 1587 1926 1530 1559 2282 3376 3525 4448 2297 465 1498 1699 2710 3941 1530 1559 2282 3376 3525 4448 620 3226 3311 4472 969 3230 4614 746 2385 2484 613 1392 1843 627 713 2267 598 1365 2231 2596 2604 4542 360 1026 1178 1565 1916 3525 3054 4062 4782 1370 2610 3055 2297 3658 3840 737 1554 2002 2646 3809 713 2267 737 1554 2002 2646 3809 969 3230 4614 360 1026 1178 1565 1916 627 1370 2610 3055 360 1026 1178 1565 1916 2231 2211 598 1365 2231 2596 2604 4542

Sequence gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg gt/mkac r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg agc/gct g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc cac/gtg gaagac gwgcw/c gaagac cgannnnnntgc c/ycgrg gccnnnn/nggc a/gatct gaagac ctggag tt/cgaa gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc gaggag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg gaatgc c/ycgrg tt/cgaa t/gtaca rgcgc/y c/catgg cgat/cg t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y

BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI CciNI Cfr10I CfrI Csp45I DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco72I Eco88I Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I ErhI Esp1396I FauNDI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I Ksp632I LspI MamI MfeI MflI MluI MluNI Mph1103I MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV

1 6 1 7 1 10 3 10 1 1 5 8 1 4 2 2 2 6 8 1 2 1 2 1 1 1 1 5 5 1 2 1 1 5 5 1 3 1 1 1 2 1 1 5 1 5 1 1 5 1 5 6 8 2 2 1 1 8 2 1 1 1 10 1 2 1 1 2 9 8 4 1 3 1 5 1 1

1548 1530 1559 2282 3376 3525 4448 2211 812 931 2121 2528 3648 4326 4517 340 585 609 1548 2180 3247 3264 4032 4044 4130 4141 978 1224 1716 585 609 1548 2180 3247 3264 4032 4044 4130 4141 2279 2279 737 1554 2002 2646 3809 756 968 1313 1401 1650 2091 2279 3501 627 2337 3316 4008 4027 1032 1790 2163 2754 2798 4680 360 1026 1178 1565 1916 2231 756 968 1313 1401 1650 2091 2279 3501 2279 1061 3105 3894 1061 3105 618 3894 2279 340 360 1026 1178 1565 1916 620 1359 1393 1737 2680 3413 1590 3827 596 2279 791 1632 2055 3212 4260 465 1498 1699 2710 3941 978 613 1392 1843 2211 618 1048 1032 1790 2211 629 360 1026 1178 1565 1916 4839 360 1026 1178 1565 1916 2006 234 620 1359 1393 1737 2680 3671 867 1050 1722 2085 3809 598 1365 2231 2596 2604 4542 122 175 258 444 1202 2012 2060 3354 2106 2398 2106 2398 622 2398 122 175 258 444 1202 2012 2060 3354 1061 3105 627 2297 2385 585 609 1548 2180 3247 3264 4032 4044 4130 4141 2521 970 1652 4839 2646 970 1652 365 976 1089 1108 1567 1915 3123 3482 3641 122 175 258 444 1202 2012 2060 3354 1532 3258 4199 4444 2385 746 2385 2484 2648 360 1026 1178 1565 1916 234 2646

g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg gc/ggccgc r/ccggy y/ggccr tt/cgaa ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc cac/gtg c/ycgrg g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc ctcttc tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Cx32
NgoMI NheI NotI NsiI NspBII NspI NspV PaeR7I PflMI PinAI Ple19I PmaCI PmlI Ppu10I PpuMI PshAI PshBI Psp124BI Psp1406I Psp5II PspEI PstI PstNHI 1 2 1 1 4 1 1 1 1 1 1 1 1 1 1 1 2 1 2 1 1 2 2 2646 591 1278 2279 4839 1532 3258 4199 4444 4786 627 613 2006 1554 3525 978 978 4835 1032 1099 7 3719 620 3292 3665 1032 2211 816 935 591 1278 g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa c/tcgag ccannnn/ntgg a/ccggt cgat/cg cac/gtg cac/gtg a/tgcat rg/gwccy gacnn/nngtc at/taat gagct/c aa/cgtt rg/gwccy g/gtnacc ctgca/g g/ctagc PvuI RcaI SacI ScaI SfcI Sfr274I SfuI SnaBI SspBI SspI SstI StyI Van91I VneI VspI XbaI XcmI XhoI XhoII XmaIII XmnI Zsp2I 1 2 1 1 7 1 1 1 2 2 1 5 1 2 2 1 2 1 10 1 1 1 3525 cgat/cg 3054 4062 t/catga 620 gagct/c 3413 agt/act 812 931 2121 2528 3648 4326 4517 c/tryag 613 c/tcgag 627 tt/cgaa 340 tac/gta 713 2267 t/gtaca 2959 3089 aat/att 620 gagct/c 360 1026 1178 1565 1916 c/cwwgg 2006 ccannnn/ntgg 3222 4468 g/tgcac 7 3719 at/taat 2289 t/ctaga 1185 2253 ccannnnn/nnnntgg 613 c/tcgag 585 609 1548 2180 3247 3264 4032 r/gatcy 4044 4130 4141 2279 c/ggccg 3294 gaann/nnttc 4839 atgca/t

The following enzymes do not cut: AatI, Acc65I, AccIII, AclNI, AflII, AocI, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BbuI, BclI, BfrI, BlnI, BlpI, Bpu1102I, Bsa29I, BscI, Bse21I, BseAI, BseCI, BsePI, BsgI, BsiMI, BsiWI, BsmBI, Bsp106I, Bsp120I, Bsp13I, Bsp1720I, Bsp68I, BspDI, BspEI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, Bsu15I, Bsu36I, CelII, Cfr42I, Cfr9I, ClaI, CpoI, CspI, CvnI, Eco147I, Eco32I, Eco81I, EcoRV, EheI, Esp3I, FbaI, FseI, KasI, Kpn2I, KpnI, Ksp22I, KspI, MroI, MspCI, NarI, NruI, PacI, PaeI, Pfl23II, Pme55I, PmeI, PspAI, PspALI, PspLI, PspOMI, PvuII, RsrII, SacII, SalI, SapI, SbfI, SexAI, SfiI, Sfr303I, SgfI, SgrAI, SmaI, SmiI, SpeI, SphI, SplI, SrfI, Sse8387I, SseBI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XmaI

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

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Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010