Alleleustrious pmTFP1-Fibrillarin

Source Catalog Number: Introduction All ustrious pmTFP1-Fibrillarin is a mammalian expression vector that expresses mTFP1 fused to a C-terminal human fibrillarin protein (FBL). This fusion protein is located in the nucleolus. The properties of All ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in the mitotic spindle of mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. Fibrilliarin is a component of small nuclear ribonucleoprotein (snRNP) and small nucleolar ribonucleoprotein (snoRNP) particle involved in pre-mRNA splicing and preribosomal RNA processing, respectively. Fibrillarin is a well conserved protein of around 320 amino acids, and contains 3 domains: an N-terminal Gly/Arg-rich region; a central RNA binding domain common among RNA-binding proteins; and a C-terminal alpha-helical domain. An evolutionarily related pre-rRNA processing protein, which lacks the Gly/Arg-rich domain, has been found in various archaebacteria. The Gly/Arg-rich domain and its variations was thought to be involved in protein-protein interactions that may be necessary for more complex RNA-protein particles.
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ABP-FP-TFIB

Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features More than 3-fold brighter than ECFP More than twice as photostable as ECFP Single emission peak Single-exponential fluorescence lifetime ( = 3.2 ns) Can be co-imaged with red and yellow FPs Low sensitivity to acidic pH (fluorescence pKa=4.3) Mammalian expression vector ready to transfect your favorite cells True monomer that will not aggregate or cause non-specific interactions Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 mTFP1 613-1320 Linker......1321-1341 Fibrillarin....................................1589-1639 SV40 Poly A Signal....................2467-2517 bla Promoter...............................3056-3160 Ampicillin Resistance Gene......3145-4005 pUC Origin..................................4154-4796 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A Fibrillarin AMPR pUC Ori PCMV

pmTFP1Fibrillarin 4878bp

mTFP1

Linker

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Annemarie Schultz, Stephanie Nottrott, Nicholas James Watkins and Reinhard Lhrmann. "Protein-Protein and Protein-RNA Contacts both Contribute to the 15.5K-Mediated Assembly of the U4/U6 snRNP and the Box C/D snoRNPs" Molecular and Cellular Biology 2006 Volume 26, pages 51465154. Wang J, Dong Z, Bell LR.. Sex-lethal interactions with protein and RNA. Roles of glycine-rich and RNA binding domains J Biol Chem. 1997 272 (35):22227-35.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Fibrillarin
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGTCGCCA ATGAAGATCA GAGGGCGAGG GCCCCCCTGC TTCACCAAGT TCTTGGGAGC TCCATGGAGG AACGGCCCCG GTGCGCGACG CACCACCGCG TATCACTTTG ACCGTTTACG TCCGGACTCA GGCCGAGGGG CGAGGCCAAG GGTGGAGGAG CGGGGAGGAA GAGGGTGTCT GGGGAATCAG TACCGAGCCT CAGATCCACA GTCTCCCATG CACCGCTCTG ATCGAGGATG TTTGCTGATG CTGCGTAATG TCAGCCGAGG CAGGAGCAGT AGGCCACCCC AGCCATACCA AACCTGAAAC GGTTACAAAT TCTAGTTGTG AAGCGCGGCG GCCCGCTCCT AGCTCTAAAT CAAAAAACTT TCGCCCTTTG AACACTCAAC CTATTGGTTA AACGCTTACA TTTTTCTAAA CAATAATATT TTTTTTGCGG GATGCTGAAG AAGATCCTTG CTGCTATGTG ATACACTATT GATGGCATGA GCCAACTTAC ATGGGGGATC AACGACGAGC ACTGGCGAAC AAAGTTGCAG TCTGGAGCCG CCCTCCCGTA AGACAGATCG TACTCATATA AAGATCCTTT GCGTCAGACC ATCTGCTGCT GAGCTACCAA GTTCTTCTAG TACCTCGCTC TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA CCATGGTGAG AGCTGAAGAT GCAAGCCCTA CCTTCTCCTA ACCCCGACGA GCACCATGAC AGGACTCCTT TGATGCAGAA GCGTGCTGAA TTGACTTCAA TGGACCACCG AGAGCGCCGT GATCTCGAGC GCTTTGGTGA GCGGAGGCTT GAGGAAGAGG AAAGAGGAAA TCATTTGTCG TTTATGGAGA GGAACCCCTT TCAAACCGGG TCTCTGACAT GCCGTGACCT CTCGACACCC TGGCCCAGCC GAGGACACTT CCGTGTTTGC TGACCCTTGA CCAAGGTGAA CATTTGTAGA ATAAAATGAA AAAGCAATAG GTTTGTCCAA GGTGTGGTGG TTCGCTTTCT CGGGGGCTCC GATTAGGGTG ACGTTGGAGT CCTATCTCGG AAAAATGAGC ATTTAGGTGG TACATTCAAA GAAAAAGGAA CATTTTGCCT ATCAGTTGGG AGAGTTTTCG GCGCGGTATT CTCAGAATGA CAGTAAGAGA TTCTGACAAC ATGTAACTCG GTGACACCAC TACTTACTCT GACCACTTCT GTGAGCGTGG TCGTAGTTAT CTGAGATAGG TACTTTAGAT TTGATAATCT CCGTAGAAAA TGCAAACAAA CTCTTTTTCC TGTAGCCGTA TGCTAATCCT TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGGGCGAG GGAGGGCAAC CGACGGCACC CGACATTCTG CATCCCCAAC CTTCGAGGAC CATCTACGAG GAAGACCACC GGGCGACGTC GACCATCTAC CATCGAGATC GGCCCGCAAC CATGAAGCCA CCGTGGTGGT TAGAGGTCGT TGGTGGAGGC CCAGTCGGGG GGGAAAGGAA GAAGAGAGTC CCGCTCCAAG GGCTAAGGTT CGTTGGTCCG CATTAACTTG ACACAAATAC AGACCAGACC TGTGATTTCC CTCCGAAGTG GCCATATGAA GAACTGAGGA GGTTTTACTT TGCAATTGTT CATCACAAAT ACTCATCAAT TTACGCGCAG TCCCTTCCTT CTTTAGGGTT ATGGTTCACG CCACGTTCTT TCTATTCTTT TGATTTAACA CACTTTTCGG TATGTATCCG GAGTATGAGT TCCTGTTTTT TGCACGAGTG CCCCGAAGAA ATCCCGTATT CTTGGTTGAG ATTATGCAGT GATCGGAGGA CCTTGATCGT GATGCCTGTA AGCTTCCCGG GCGCTCGGCC GTCTCGCGGT CTACACGACG TGCCTCACTG TGATTTAAAA CATGACCAAA GATCAAAGGA AAAACCACCG GAAGGTAACT GTTAGGCCAC GTTACCAGTG ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC GAGACCACAA GTGAATGGCC AACACCATCA ACCACCGCGT TACTTCAAGC AAGGGCATCG ATACACCTCA GGCTGGGACG AAGCACAAGC AGGGCCAAGA CTGAACCACG TCCACCGACG GGATTCAGTC CGTGGAGGCC GGACGAGGAG TTCCATTTTG AAGAATGTGA GATGCACTGG TCGATTTCGG CTAGCAGCAG CTCTACCTCG GATGGTCTAG GCCAAGAAGA CGCATGCTCA CGGATTGTGG ATTAAGGCCA AAAAAGATGC AGAGACCATG TCCACCGGAT GCTTTAAAAA GTTGTTAACT TTCACAAATA GTATCTTAAC CGTGACCGCT TCTCGCCACG CCGATTTAGT TAGTGGGCCA TAATAGTGGA TGATTTATAA AAAATTTAAC GGAAATGTGC CTCATGAGAC ATTCAACATT GCTCACCCAG GGTTACATCG CGTTTTCCAA GACGCCGGGC TACTCACCAG GCTGCCATAA CCGAAGGAGC TGGGAACCGG GCAATGGCAA CAACAATTAA CTTCCGGCTG ATCATTGCAG GGGAGTCAGG ATTAAGCATT CTTCATTTTT ATCCCTTAAC TCTTCTTGAG CTACCAGCGG GGCTTCAGCA CACTTCAAGA GCTGCTGCCA AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC TGGGCGTAAT ACGCCTTCGT ACCTGGAGGT TCGCCTACGG AGTCCTTCCC TGAAGGTGAA AGGGCGAGAA CCTCCACCGA TGCTGCTGGA AGGCGGTGAA ACAAGGACTA GCATGGACGA CCCGTGGGGG GAGGGGGCTT GAGGTGGAGG GTGGCAACCG TGGTGGAGCC TCACCAAGAA AAGGAGATGA CAATCCTGGG GGGCTGCCTC TCTATGCAGT GGACCAACAT TCGCAATGGT CCCTGAATGC ACTGCATTGA AACAGGAGAA CCGTGGTCGT CTAGATAACT ACCTCCCACA TGTTTATTGC AAGCATTTTT GCGTCCTGTA ACACTTGCCA TTCGCCGGCT GCTTTACGGC TCGCCCTGAT CTCTTGTTCC GGGATTTTGC GCGAATTTTA GCGGAACCCC AATAACCCTG TCCGTGTCGC AAACGCTGGT AACTGGATCT TGATGAGCAC AAGAGCAACT TCACAGAAAA CCATGAGTGA TAACCGCTTT AGCTGAATGA CAACGTTGCG TAGACTGGAT GCTGGTTTAT CACTGGGGCC CAACTATGGA GGTAACTGTC AATTTAAAAG GTGAGTTTTC ATCCTTTTTT TGGTTTGTTT GAGCGCAGAT ACTCTGTAGC GTGGCGATAA TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CAAGCCCGAC GATCGAGGGC GAAGGAGGGA CAACAGGGCC CGAGGGCTAC GTCCGACATC CTTCCCCCCC GAGGATGTAC GGGCGGCGGC GCTGCCCGAC CAACAAGGTG GCTGTACAAG TGGCTTTGGC TGGCGGGGGC CGGCGGCGGC GGGTCGTGGT GCATCGGCAT CCTGGTCCCT CAAAATTGAG TGGTGTGGAC GGGCACCACG CGAGTTCTCC CATTCCTGTG GGATGTGATC CCACACCTTC CTCCACAGCC CATGAAGCCG GGGAGTGTAC GATCATAATC CCTCCCCCTG AGCTTATAAT TTCACTGCAT GCGGCGCATT GCGCCCTAGC TTCCCCGTCA ACCTCGACCC AGACGGTTTT AAACTGGAAC CGATTTCGGC ACAAAATATT TATTTGTTTA ATAAATGCTT CCTTATTCCC GAAAGTAAAA CAACAGCGGT TTTTAAAGTT CGGTCGCCGC GCATCTTACG TAACACTGCG TTTGCACAAC AGCCATACCA CAAACTATTA GGAGGCGGAT TGCTGATAAA AGATGGTAAG TGAACGAAAT AGACCAAGTT GATCTAGGTG GTTCCACTGA TCTGCGCGTA GCCGGATCAA ACCAAATACT ACCGCCTACA GTCGTGTCTT

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Fibrillarin
4441 4501 4561 4621 4681 4741 4801 4861 ACCGGGTTGG GGTTCGTGCA CGTGAGCTAT AGCGGCAGGG CTTTATAGTC TCAGGGGGGC TTTTGCTGGC CGTATTACCG ACTCAAGACG CACAGCCCAG GAGAAAGCGC TCGGAACAGG CTGTCGGGTT GGAGCCTATG CTTTTGCTCA CCATGCA ATAGTTACCG CTTGGAGCGA CACGCTTCCC AGAGCGCACG TCGCCACCTC GAAAAACGCC CATGTTCTTT GATAAGGCGC ACGACCTACA GAAGGGAGAA AGGGAGCTTC TGACTTGAGC AGCAACGCGG CCTGCGTTAT AGCGGTCGGG CCGAACTGAG AGGCGGACAG CAGGGGGAAA GTCGATTTTT CCTTTTTACG CCCCTGATTC CTGAACGGGG ATACCTACAG GTATCCGGTA CGCCTGGTAT GTGATGCTCG GTTCCTGGCC TGTGGATAAC

Enzyme AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I Aor51HI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbsI BbuI Bbv12I Bbv16II BcgI BclI BcoI BglI BglII BpiI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseAI BseRI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiMI BsmI BsoBI Bsp13I Bsp1407I Bsp143II Bsp19I BspCI BspEI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I

Cuts 5 1 1 5 2 4 2 3 7 1 2 1 1 3 2 1 4 1 2 3 2 2 2 1 3 4 1 2 1 5 2 7 2 1 3 2 2 1 4 8 1 2 3 2 3 2 7 4 3 5 7 4 2 2 4 5 2 5 5 3 2 2 3 4 2 2 5 3 1 2 2 1 4 2 3 4 2 4 2 5

Positions 125 178 261 447 1109 3451 3709 465 745 1852 2748 3979 1052 2262 1766 1928 2648 3093 1321 1887 2487 2972 2983 122 175 258 444 1058 1106 3392 596 2559 4820 600 3932 3264 3349 4510 3260 4506 4411 889 1334 1837 1848 596 3260 4506 2487 2972 2983 7 3757 7 3757 1756 3332 3932 3264 3349 4510 889 1334 1837 1848 3709 698 1951 2308 465 745 1852 2748 3979 783 2718 122 175 258 444 1058 1106 3392 1046 1632 2016 3264 3349 4510 1046 1632 2023 3416 2330 889 1334 1837 1848 90 212 283 1180 1388 1424 1614 3814 1330 1046 1632 768 1131 3847 1046 1632 340 1080 2789 1807 2335 122 175 258 444 1058 1106 3392 636 1865 2257 3865 2090 2423 2522 605 1383 3414 3563 4486 600 1321 1887 2314 3636 4467 4614 600 1048 2684 3847 1807 2335 1321 1887 633 972 1480 1510 605 1383 3414 3563 4486 1807 2335 465 745 1852 2748 3979 605 1383 3414 3563 4486 3264 3349 4510 3268 4652 1321 1887 2090 2423 2522 889 1334 1837 1848 1321 1887 1313 2276 598 1277 2634 2642 4580 360 611 962 3563 1321 1887 3092 4100 4820 1766 1928 2648 3093 1807 2335 2028 3696 3878 600 1048 2684 3847 1313 2276 600 1048 2684 3847 3268 4652 360 611 962 1446 2291

Sequence gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg agc/gct g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc gaagac gcatg/c gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc a/gatct gaagac ctggag gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc t/ccgga gaggag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg t/ccgga gaatgc c/ycgrg t/ccgga t/gtaca rgcgc/y c/catgg cgat/cg t/ccgga t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg

BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI Cfr10I CfrI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI EclHKI EclXI Eco105I Eco130I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco88I Eco91I EcoO109I EcoO65I EcoT14I EcoT22I ErhI Esp1396I FauNDI FbaI FriOI FspI GsuI HaeII Hin1I HincII HindII HpaI Hsp92I Kpn2I Ksp22I Ksp632I MamI MfeI MflI MluI MluNI Mph1103I MroI MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI

4 8 3 5 1 5 3 5 1 12 4 12 1 4 6 4 1 3 2 8 6 1 4 1 4 1 1 1 5 2 1 4 1 1 4 5 4 3 1 3 5 1 5 2 2 1 2 1 3 5 7 3 3 1 7 2 1 4 2 1 12 1 2 1 2 1 2 10 7 3 1 3 1 3 2 1 1

1766 1928 2648 3093 360 611 962 1277 1362 1401 1856 2261 1257 1396 1659 598 1277 2634 2642 4580 2308 605 1383 3414 3563 4486 1257 1396 1659 1167 2566 3686 4364 4555 340 585 1226 1330 1802 2308 2317 3285 3302 4070 4082 4168 4179 762 1540 1671 1927 585 1226 1330 1802 2308 2317 3285 3302 4070 4082 4168 4179 1380 600 1048 2684 3847 696 1137 1380 1929 1949 3539 2375 3354 4046 4065 836 1209 2121 2792 2836 4718 360 611 962 1277 1362 1401 1856 2261 696 1137 1380 1929 1949 3539 1380 1519 1706 1961 3143 3932 1519 1706 1961 3143 3932 1380 340 360 611 962 1446 2291 783 2718 3451 636 1865 2257 3865 596 1380 678 1101 3250 4298 465 745 1852 2748 3979 889 1334 1837 1848 1257 1396 1659 836 1257 1396 1659 360 611 962 1446 2291 4877 360 611 962 1446 2291 1052 2262 234 2244 2330 783 2718 3709 768 1131 3847 598 1277 2634 2642 4580 122 175 258 444 1058 1106 3392 1152 2231 2436 1152 2231 2436 2436 122 175 258 444 1058 1106 3392 1321 1887 2330 1519 1706 1961 3143 1807 2335 2423 585 1226 1330 1802 2308 2317 3285 3302 4070 4082 4168 4179 2559 698 1951 4877 1321 1887 2684 698 1951 365 613 961 1538 1616 1975 2023 3161 3520 3679 122 175 258 444 1058 1106 3392 3296 4237 4482 2423 2090 2423 2522 2686 360 611 962 234 2244 2684 2684

gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg r/ccggy y/ggccr ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gacnnn/nngtc c/ggccg tac/gta c/cwwgg grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc c/ycgrg g/gtnacc rg/gnccy g/gtnacc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg t/gatca grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac gtt/aac gr/cgyc t/ccgga t/gatca ctcttc gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Fibrillarin
NheI NsiI NspBII NspI PaeI PaeR7I PflMI PinAI Ple19I Ppu10I PshBI Psp1406I PspEI PstNHI PvuI RcaI ScaI SexAI 2 1 3 2 1 1 2 1 1 1 2 2 3 2 1 2 1 1 591 1770 4877 3296 4237 4482 2016 4824 2016 1334 1052 2262 600 3563 4873 7 3757 3330 3703 1257 1396 1659 591 1770 3563 3092 4100 3451 1670 g/ctagc atgca/t cmg/ckg rcatg/y gcatg/c c/tcgag ccannnn/ntgg a/ccggt cgat/cg a/tgcat at/taat aa/cgtt g/gtnacc g/ctagc cgat/cg t/catga agt/act a/ccwggt SfcI Sfr274I SnaBI SphI SspBI SspI StyI Van91I VneI VspI XbaI XcmI XhoI XhoII XmaIII XmnI Zsp2I 5 1 1 1 2 2 5 2 2 2 1 1 1 12 1 2 1 1167 2566 3686 4364 4555 1334 340 2016 1313 2276 2997 3127 360 611 962 1446 2291 1052 2262 3260 4506 7 3757 2320 1299 1334 585 1226 1330 1802 2308 2317 3285 3302 4070 4082 4168 1380 1756 3332 4877 c/tryag c/tcgag tac/gta gcatg/c t/gtaca aat/att c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy 4179 c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: AatI, Acc65I, AccI, AclNI, AflII, AocI, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BfrI, BlnI, BlpI, Bpu1102I, Bpu14I, Bsa29I, BscI, Bse21I, BseCI, BsePI, BsgI, BsiWI, BsmBI, Bsp106I, Bsp119I, Bsp120I, Bsp1720I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstBI, Bsu15I, Bsu36I, CciNI, CelII, Cfr42I, Cfr9I, ClaI, CpoI, Csp45I, CspI, CvnI, Ecl136II, Eco147I, Eco32I, Eco72I, Eco81I, EcoICRI, EcoNI, EcoRI, EcoRV, EheI, Esp3I, FseI, HindIII, KasI, KpnI, KspI, LspI, MspCI, NarI, NotI, NruI, NspV, PacI, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp124BI, Psp5II, PspAI, PspALI, PspLI, PspOMI, PstI, PvuII, RsrII, SacI, SacII, SalI, SapI, SbfI, SfiI, Sfr303I, SfuI, SgfI, SgrAI, SmaI, SmiI, SpeI, SplI, SrfI, Sse8387I, SseBI, SstI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XmaI

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

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Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010