BIOL 437

DNA Repair and Mutagenesis

Lecture 1
Textbook Chapter 1-3
September 10, 2009

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 Class Announcements
Submit Assignment 1 by 18:00 on the 17th.
Check your student number to be posted by the
17th.
Consider Assignment 2 and an exercise to be
prepared for the Term Paper.
Future postings of both ppt and jpeg files.
Go to slide 19

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Mutations: Key Definitions

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Types of Point Mutations

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Second Site Events (example)

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Terms characterizing mutations

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Reversion of a FS Mutation

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T4 rII Gene Mutation

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AMES TEST

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MFO

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Forward Mutagenesis: The LACI System

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Mutation is Non-Random

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Forward Mutation Systems

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 SupF secondary Structure

The secondary structure may or may not be important for individual

events. It does allow the sequence to be displayed in a small box!
These mutations allows the selection of mutations suppressing an
event elsewhere such as in a lacZ fs mutation.
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SHUTTLE VECTORS

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Shuttle Vector Protocols

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Mutation in Mammalian Cells
Direct Sequencing

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Mutation in vivo: the laci system

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 Site Specific Adducts – An
alternative use for shuttle vectors

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 Mutation is Targeted
Evidence
Site specific data
Mutational specificity changes with agent
Photoreveral of some lesions
And...

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Mutagenic Misalignment
Intermediates

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 Sequence Directed Mutation
Tandem Repeats and Palimdromes

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Complex Sequence Directed Mutation
(Bacteriophage T4rII)

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 Reading Assignment
The next lecture is about UV damage and mutation. Please
read the textbook (Chapter 3) and then the two papers
posted under references on the website. These will be:
1) Glickman et al. (1986) The C-C (6-4) photoproduct is
mutagenic in Escherichia coli. Proc Natl. Acad. Sci. (USA)
83, 6945-49.
2) Drobetsky et al (1987) The specificity of UV-induced
mutations at an endogenous locus in mammalian cells.
Proc Natl Acad Sci (USA) 84.24 (1987): 9103-07.

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 Next Topics
We will continue with the repair of base damage.
This is described in Part 2 of the textbook. We
will start with Chapters 4 and 5 and progress
sequentially through this section of the book.
Chapter 4: Reversal of base damage caused by
UV.
Chapter 5: Reversal of alkylation damage in DNA

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