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    Cody Burgett
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    The document describes an Enterotube II test used to identify enteric bacteria based on their ability to ferment different sugars such as glucose, lactose, and sorbitol. The test involves inoculating an Enterotube II with E. coli culture using a needle to introduce the bacteria into compartments containing different sugars. The tube is then incubated and reagents are added to identify the bacteria based on the fermentation codes.

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    The document describes an Enterotube II test used to identify enteric bacteria based on their ability to ferment different sugars such as glucose, lactose, and sorbitol. The test involves inoculating an Enterotube II with E. coli culture using a needle to introduce the bacteria into compartments containing different sugars. The tube is then incubated and reagents are added to identify the bacteria based on the fermentation codes.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    Download as docx, pdf, or txt
    294 views1 page

    Enterotube II

    Uploaded by

    Cody Burgett
    The document describes an Enterotube II test used to identify enteric bacteria based on their ability to ferment different sugars such as glucose, lactose, and sorbitol. The test involves inoculating an Enterotube II with E. coli culture using a needle to introduce the bacteria into compartments containing different sugars. The tube is then incubated and reagents are added to identify the bacteria based on the fermentation codes.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    of 1

    Enterotube II

    11/2/2010

    Section 2, Bench 3

    Introduction: The enterotube II is a multiple test system designed to identify enteric bacteria based on:
    glucose, adonitol, lactose, arabinose, sorbitol, and dulcitol fermentation. A code is used for each
    compartment. This code identifies the bacteria.

    Materials:

    Enterotube II
    Sterile culture of E.coli

    Methods

    1. Inoculate enterotube needle with E.coli.


    2. Pull needle through tube while twisting.
    3. Reinsert need through tube.
    4. Break needle off at line, puncture air holes.
    5. Incubate at 37* for 24-48 hours.
    6. Add reagents necessary.

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