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    An Intrinsic Control Elemen..

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    The document discusses how translation of an aminoglycoside 6'-N-acetyltransferase gene (aac(6')-Ib7) inserted at the attI1 site of integrons is initiated at a GTG codon, rather than a plausible translation initiation region. Through site-directed mutagenesis of a short open reading frame (ORF-11) that overlaps the attI1 site, the researchers found that deletion of ORF-11 and its translation initiation region decreased translational efficiency of the acetyltransferase gene by over 80%. Mutation of the start codon or Shine-Dalgarno sequence of ORF-11 also significantly decreased translation, demonstrating that expression of the acetyltransferase gene is

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    An Intrinsic Control Elemen..

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    b12071973
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    The document discusses how translation of an aminoglycoside 6'-N-acetyltransferase gene (aac(6')-Ib7) inserted at the attI1 site of integrons is initiated at a GTG codon, rather than a plausible translation initiation region. Through site-directed mutagenesis of a short open reading frame (ORF-11) that overlaps the attI1 site, the researchers found that deletion of ORF-11 and its translation initiation region decreased translational efficiency of the acetyltransferase gene by over 80%. Mutation of the start codon or Shine-Dalgarno sequence of ORF-11 also significantly decreased translation, demonstrating that expression of the acetyltransferase gene is

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    The document discusses how translation of an aminoglycoside 6'-N-acetyltransferase gene (aac(6')-Ib7) inserted at the attI1 site of integrons is initiated at a GTG codon, rather than a plausible translation initiation region. Through site-directed mutagenesis of a short open reading frame (ORF-11) that overlaps the attI1 site, the researchers found that deletion of ORF-11 and its translation initiation region decreased translational efficiency of the acetyltransferase gene by over 80%. Mutation of the start codon or Shine-Dalgarno sequence of ORF-11 also significantly decreased translation, demonstrating that expression of the acetyltransferase gene is

    Copyright:

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    8 views1 page

    An Intrinsic Control Elemen..

    Uploaded by

    b12071973
    The document discusses how translation of an aminoglycoside 6'-N-acetyltransferase gene (aac(6')-Ib7) inserted at the attI1 site of integrons is initiated at a GTG codon, rather than a plausible translation initiation region. Through site-directed mutagenesis of a short open reading frame (ORF-11) that overlaps the attI1 site, the researchers found that deletion of ORF-11 and its translation initiation region decreased translational efficiency of the acetyltransferase gene by over 80%. Mutation of the start codon or Shine-Dalgarno sequence of ORF-11 also significantly decreased translation, demonstrating that expression of the acetyltransferase gene is

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    of 1

    An intrinsic control element for translational ini... [Mol Microbiol. 2002] ... http://www.ncbi.nlm.nih.

    gov/pubmed/11967073

    PubMed
    U.S. National Library of Medicine
    National Institutes of Health

    Display Settings: Abstract

    Mol Microbiol. 2002 Apr;44(1):119-30.

    An intrinsic control element for translational initiation in class 1


    integrons.
    Hanau-Berçot B, Podglajen I, Casin I, Collatz E.
    INSERM EMI 0004 - LRMA, Université Paris VI, 15, rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France.

    Abstract
    Integrons are genetic elements able to capture anti-biotic resistance and other genes and to promote their transcription.
    Here, we have investigated integron-dependent translation of an aminoglycoside 6'-N-acetyltransferase gene (aac(6')-Ib7)
    inserted at the attI1 site. N-terminal sequencing revealed that translation of this gene was initiated at a GTG codon, which
    is not part of a plausible translation initiation region (TIR). A short open reading frame (called ORF-11) overlapping the
    attI1 site was probed by site-directed mutagenesis for its contribution to aac(6')-Ib7 translation. When ORF-11 and its TIR
    were deleted en bloc, translational efficiency dropped by over 80%, as determined with an acetyltransferase- luciferase
    fusion product. Invalidation of the ATG start codon of ORF-11 or its putative Shine-Dalgarno sequence resulted in a
    decrease of over 60%, whereas the decrease was much less pronounced when the amino acid sequence of the putative
    ORF-11-encoded peptide was altered or when the distance between ORF-11 and aac(6')-Ib7 was doubled. This
    demonstrates that aac(6')-Ib7 translation is dependent upon the translation of ORF-11, but almost certainly not upon the
    corresponding peptide. These results lead us to conclude that an intrinsic short ORF present in the 5'-conserved segment
    of many class 1 integrons may substantially enhance expression at the translational level of captured TIR-deficient
    anti-biotic resistance genes.

    PMID: 11967073 [PubMed - indexed for MEDLINE]

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