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Zoolab 4
Zoolab 4
Activity #4
Objectives:
Detergent Ethanol
Salt Distilled water
2 test tubes 1 test tube rack
1 stirring rod Plastic cup
Procedures:
First in to collect some of the buccal cells that line the inside of the mouth. Swill 2
teaspoons of salt watr in our mouth for 30 seconds.
Step two spit the water in to your cup. Pour this into the test tube cantaning 1 teaspoon of
liquid detergentThen gently rock it on its side for 2-3 minutes. The detergent will treak open
the cell membrane to release the DNA into the soap solution.open and s;ightly tilt the tube
and pour 1 teaspoon of ethanol down the side of the tube so that it forms a layer on the top
of your soap solution.allow the tube to stand for 1 minute.
Place a thin glasrod into the tube then stir the rod in one direction to wind the DNA starnds
onto the rod.Be careful to minimize mixing of the eathnol and soap layer.
Raymoon C. Flores
Ino Vincent E. Espiritusanto
BS Psychology 4
Zoolab 101 B