Reference No:001 Year: 1 COSHH:b0y03: 01 Experiment: Starch Agar Plates To Prepare in Advance by Tech: Method

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Reference No:001 Module: 1 Year: 1 COSHH:b0y03: 01

Experiment: Starch agar plates


To prepare in advance by tech: Method
 Starch agar plates Starch agar plates 1. 5g starch in total volume 500mls.
 Barley seeds to germinate (week before) Make starch solution (200mls) 2. Make agar: 14g nutrient
 Yeast solution (48hrs) 25 degree centigrade) agar +teaspoon of agar powder into small amount of water
heat to about 90c in water bath then cool to 55c. 3.Mix
solutions and make up 500mls. 4. Autoclave and pour
plates.
Items required by class: `
 Plates
 Barley
 Yeast
 Distilled water
 Cork borers
 Dropping pipettes
 Scalpels
 Labels
 Bowl of disinfectant for beakers and pipettes with saliva.
 Cutting tiles
Notes:
 Plates need to be incubated at 25 c till next lesson.
 Iodine needed next lesson
 Autoclave plates after use.

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