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    TLC Thin Layer Chromatography: Technique Employed For The Qualitative Analysis of Mixtures and Pure Organic Compounds

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    cybermaru
    Thin layer chromatography (TLC) is a technique used to qualitatively analyze mixtures and pure organic compounds. TLC separates components in a mixture based on their relative affinities for the mobile and stationary phases. The stationary phase is a polar solid adsorbent coated as a thin layer on a glass or plastic sheet. The mobile phase is an appropriate solvent that carries the mixture's components according to their relative adsorption affinities up the plate via capillary action. Therefore, the components are arranged from most polar at the bottom to least polar at the top of the developed plate. A TLC experiment involves sample application by spotting onto the plate, chromatographic development using the mobile phase, and visualization of the results

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    TLC Thin Layer Chromatography: Technique Employed For The Qualitative Analysis of Mixtures and Pure Organic Compounds

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    cybermaru
    36 views8 pages
    Thin layer chromatography (TLC) is a technique used to qualitatively analyze mixtures and pure organic compounds. TLC separates components in a mixture based on their relative affinities for the mobile and stationary phases. The stationary phase is a polar solid adsorbent coated as a thin layer on a glass or plastic sheet. The mobile phase is an appropriate solvent that carries the mixture's components according to their relative adsorption affinities up the plate via capillary action. Therefore, the components are arranged from most polar at the bottom to least polar at the top of the developed plate. A TLC experiment involves sample application by spotting onto the plate, chromatographic development using the mobile phase, and visualization of the results

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    TLC

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    Thin layer chromatography (TLC) is a technique used to qualitatively analyze mixtures and pure organic compounds. TLC separates components in a mixture based on their relative affinities for the mobile and stationary phases. The stationary phase is a polar solid adsorbent coated as a thin layer on a glass or plastic sheet. The mobile phase is an appropriate solvent that carries the mixture's components according to their relative adsorption affinities up the plate via capillary action. Therefore, the components are arranged from most polar at the bottom to least polar at the top of the developed plate. A TLC experiment involves sample application by spotting onto the plate, chromatographic development using the mobile phase, and visualization of the results

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    Download as ppt, pdf, or txt
    36 views8 pages

    TLC Thin Layer Chromatography: Technique Employed For The Qualitative Analysis of Mixtures and Pure Organic Compounds

    Uploaded by

    cybermaru
    Thin layer chromatography (TLC) is a technique used to qualitatively analyze mixtures and pure organic compounds. TLC separates components in a mixture based on their relative affinities for the mobile and stationary phases. The stationary phase is a polar solid adsorbent coated as a thin layer on a glass or plastic sheet. The mobile phase is an appropriate solvent that carries the mixture's components according to their relative adsorption affinities up the plate via capillary action. Therefore, the components are arranged from most polar at the bottom to least polar at the top of the developed plate. A TLC experiment involves sample application by spotting onto the plate, chromatographic development using the mobile phase, and visualization of the results

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    TLC =

    Thin Layer Chromatography

    technique employed for the qualitative


    analysis of mixtures and pure organic
    compounds
    Thin Layer Chromatography

    CHROMATOGRPAHY

    components in a mixture are separated


    based on their relative affinities for the
    mobile phase and stationary phase
    Thin Layer Chromatography

    stationary phase =
    a polar solid adsorbant such as silica gel
    or alumina coated as a thin layer on the
    surface of a sheet of GLASS or PLASTIC
    Thin Layer Chromatography

    mobile phase =
    an appropriate solvent depending on
    your sample, for example 50% ethyl
    acetate, 30% denatured alcohol, 20%
    water
    Thin Layer Chromatography

    the solvent travels up the plate by


    capillary action and the various
    components in the mixture are carried
    by the solvent according to their relative
    adsorption affinites
    Thin Layer Chromatography

    Therefore, the More polar


    components of a
    mixture will be
    arranged in order
    of increasing
    polarity from top
    to bottom of the
    developed plate Less polar
    Thin Layer Chromatography

    TLC experiment has 3 parts:

    • sample application
    • chromatographic development
    • visualization
    Thin Layer Chromatography

    Sample application

    samples are “spotted” onto a TLC


    plate using micropipets

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