Recombinant DNA

Technology

By: Yogesh Vaskar

Bhavesh rathod
 Content
Introduction
Application of rDNA Tecnology
a. Colony Hybridization
b.DNA Sequencing
c. PCR
d.Southern Blotting
e. Gene Therapy
f. Diagnosis of Diseases
g.Vaccines
h.Insulin Production
 Intoduction
Recombinant DNA (rDNA) is a form of artificial
DNA that is created by combining two or more
sequences that would not normally occur
together through the process of gene splicing.

In terms of genetic modification, it is created

through the introduction of relevant DNA into an
existing organismal DNA, such as the plasmids of
bacteria, to code for or alter different traits for a
specific purpose, such as antibiotic resistance.
Colony Hybridization
DNA Sequencing
PCR

What is PCR??
Southern Blotting
GENE THERAPY

SOMATIC GENE THERAPY

GERM-LINE GENE THERAPY

Methods of Gene Transfer
1. Microinjection

2. Electroporation

3. Retroviruses

4. Injection of Liposomes
Diagnosis of Genetic
Diseases

 Cystic Fibrosis

 Sickle-Cell Anemia
Vaccines
 Hepatitis B – Vaccine
Insulin Production for Human
Use