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Metabolic Pathways: A. What Is Metabolism?
Metabolic Pathways: A. What Is Metabolism?
METABOLIC PATHWAYS
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A. WHAT IS METABOLISM?
1. Anabolism (construction)
2. Catabolism (destruction, demolition)
B. CARBOHYDRATE METABOLISM
1. Synopsis
2. Glycolysis (early steps)
3. Glycolysis (later steps)
4. Thiamine/Lipoic Acid "Connector"
5. Krebs Cycle
6. Starch/Glycogen
7. Controls on Lactase/β-galactosidase activity
8. Lactose
9. ONPG (o-nitrophenyl-ß-D-galactopyranoside)
10. Pentose Shunt
C. ELECTRON TRANSPORT
1. Cytochrome level Electron Transport
2. Catalase Reaction
D. PHOTOSYNTHESIS
1. Transpiration
E. THE NITROGEN POOL: UREA CYCLE, AMINO ACID & PROTEIN
SYNTHESIS
1. Selected genes
a. the rRNA gene
b. the lac-operon
2. Translation
3. Protein Primary Structure (modelling aids)
4. Post-Translational Modifications
F. LIPID METABOLISM
1. Fats (tri-glycerides)
2. Fatty Acids
3. Steroids: are made from squalene in the following order (male mammals cannot
go beyond testosterone):
a. cholesterol
b. testosterone
c. ß-estradiol
G. Poly- and mono-NUCLEOTIDE METABOLISM
1. Shape of prokaryotic genome: a "(supercoil)"
2. Replicating prokaryotic chromosome
3. Synthesis of DNA and RNA
4. Catabolism of DNA and RNA
5. Nucleoside Synthesis
a. Purine biosynthesis:
i. To inosinic acid
ii. From Inosinic acid to AMP and GMP
b. Uracil Synthesis (UMP)
c. deoxynucleotide synthesis
d. TMP synthesis
e. HMC synthesis in T-even coliphages
6. Nucleotide Catabolism
a. Purine catabolism
b. Pyrimidine catabolism
H. PROTEIN METABOLISM|